A5101605666- Sperm and Testicular Function
- Reproductive Biology and Fertility
- Reproductive Health and Technologies
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Sexual Differentiation and Disorders
- MicroRNA in disease regulation
- Ovarian function and disorders
- Hormonal and reproductive studies
- Ferroptosis and cancer prognosis
- Testicular diseases and treatments
- Epigenetics and DNA Methylation
- Microtubule and mitosis dynamics
- Graphene and Nanomaterials Applications
- RNA and protein synthesis mechanisms
- CRISPR and Genetic Engineering
- Microplastics and Plastic Pollution
- Liver Disease and Transplantation
- biodegradable polymer synthesis and properties
- Hepatitis B Virus Studies
- Hepatitis Viruses Studies and Epidemiology
- Circular RNAs in diseases
- Pneumocystis jirovecii pneumonia detection and treatment
- Heat shock proteins research
- Complement system in diseases
- Cognitive Science and Education Research
Peking University First Hospital
2023-2025
Peking University
2017-2025
Chinese People's Liberation Army
2025
Peking University Third Hospital
2017-2024
Tsinghua University
2006-2024
Center for Life Sciences
2019-2024
Shanghai Harbour Engineering Design & Research Institute
2024
Sichuan University
2023
Yanshan University
2023
Yunnan University
2023
Aging-related fertility decline is a prevalent concern globally. Male reproductive system aging mainly characterized by decrease in sperm quality and fertility. While it known that intestinal physiology changes with age microbiota shaped physiology, the underlying mechanism of how affects male still largely unexplored.Here, we utilized fecal transplantation (FMT) to exchange between young old mice. Cecal shotgun metagenomics metabolomics were used identify differences gut composition...
Globally, hepatitis E virus (HEV) infections are prevalent. The finding of high viral loads and persistent shedding in ejaculate suggests that HEV replicates within the human male genital tract, but its target organ is unknown appropriate models lacking. We aimed to determine tropism testis potential influence on reproductive health. conducted ex vivo culture explants vitro primary Sertoli cells. Clinically derived genotype 1 (HEV1) HEV3 virions, as well rat HEV-C1, were used for...
Although considered an "eco-friendly" biodegradable plastic, polylactic acid (PLA) microplastic (PLA-MP) poses a growing concern for human health, yet its effects on male reproductive function remain underexplored. This study investigated the toxicity of PLA in mice and potential mechanisms. To this end, our vivo vitro experiments demonstrated that after degradation digestive system, significant number PLA-MP-derived nanoparticles could penetrate blood–testis barrier (BTB) localize within...
A total of 112 carriers (51 males and 61 females) with Robertsonian translocations who underwent 148 cycles preimplantation genetic testing for structural rearrangements (PGT-SR) between February 2017 December 2023 at the First Center General Hospital Chinese PLA were recruited. The heterologous type rob (13; 14) was most common form translocation, which accounted 65.2% (73/112). euploidy rate blastocysts 42.1% (303/720). Male had higher euploid embryo than female [50.0% (149/298) vs 36.5%...
Many studies have shown that microRNAs (miRNAs) play vital roles during the spermatogenesis. However, little is known about altered miRNA profiles of testicular tissues in nonobstructive azoospermia (NOA). Using microarray technology, expression biopsies from patients with NOA and normal were determined. Bioinformatics analyses conducted to predict enriched biological processes functions identified miRNAs. The data validated by quantitative reverse transcriptase polymerase chain reaction...
Microdissection testicular sperm extraction (micro-TESE) is widely used to treat nonobstructive azoospermia. However, a good prediction model required anticipate successful retrieval rate before performing micro-TESE. This retrospective study analyzed the clinical records of 200 azoospermia patients between January 2021 and December 2021. The backward method was perform binary logistic regression analysis identify factors that predicted micro-TESE retrieval. constructed using acquired...
Chimeric antigen receptor (CAR) T therapies have achieved remarkable success for treating hematologic malignancies, yet are often accompanied by severe cytokine release syndrome (CRS). Here, an accidental clinical observation raised the possibility that metoprolol, FDA-approved β1 adrenergic blocker widely used cardiovascular conditions, may alleviate CAR T–induced CRS. Metoprolol effectively blocked IL-6 production in human monocytes through unexpected mechanisms of action targeting protein...
Background: The lack of clinically useful biomarkers for predicting micro-TESE outcomes in males with idiopathic NOA. To find reliable serum reproductive hormone markers to predict the outcome sperm retrieval men NOA undergoing micro-TESE. Methods: We analyzed clinical data 168 treated predictors a successful were put test using logistic regression analysis. net benefit was calculated decision-curve analysis, and predictive power each variable assessed ROC-derived area under curve. Result:...
Stem cell therapy is a potentially promising option for erectile dysfunction; however, its risk of tumorigenicity clinical hurdle and the positively related to number injected cells. Our previous study showed that nanotechnology improved adipose-derived stem (ADSC) dysfunction cavernous nerve injury (CNI) by attracting cells in corpus cavernosum. These results indicated possibility using reduced dosage ADSCs intracavernous injection. In this exploratory study, we used lower (2 × 105 cells)...
Male infertility is considered a common health problem, and non-obstructive azoospermia with unclear pathogenesis one of the most challenging tasks for clinicians. The objective this study was to investigate differential serum metabolic pattern in azoospermic men determine potential biomarkers related spermatogenic dysfunction. Serum samples from patients (n = 22) healthy controls 31) were examined using high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS)....
Circular RNAs (circRNAs) are highly conserved and ubiquitously expressed noncoding that participate in multiple reproduction-related diseases. However, the expression pattern potential functions of circRNAs testes patients with non-obstructive azoospermia (NOA) remain elusive. In this study, according to a circRNA array, total 37 881 were identified differentially NOA compared normal controls, including 19 874 upregulated 18 007 downregulated circRNAs. Using quantitative real-time polymerase...
RNA-guided Streptococcus pyogenes Cas9 (SpCas9) is a sequence-specific DNA endonuclease that works as one of the most powerful genetic editing tools. However, how locates its target among huge amounts dsDNAs remains elusive. Here, combining biochemical and single-molecule fluorescence assays, we revealed uses both three-dimensional one-dimensional diffusion to find with high efficiency. We further observed surprising apparent asymmetric search regions flanking PAM sites on dsDNA under...
The coronavirus disease 2019 (COVID-19) outbreak has had a widespread and profound impact on people's mental health. factors associated with symptoms among men diagnosed infertility, closely related to psychological conditions, remain unclear. aim of this study is investigate the risk infertile Chinese during pandemic. A total 4,098 eligible participants were recruited in cross-sectional, nationwide study, including 2,034 (49.6%) primary infertility 2,064 (50.4%) secondary infertility....
Acrolein has been reported to have diverse toxic effects on various organs, including the reproductive system. However, little is known regarding of maternal acrolein exposure testicular steroidogenesis in male offspring. The present study investigated fetal testosterone production and associated genes. Pregnant Sprague‑Dawley rats were intraperitoneally injected with vehicle (normal saline) or 1, 2 5 mg/kg from gestational day (GD) 14‑20, testes examined GD 21. Fetal body weights markedly...
This study aims to investigate the gut microbiota and metabolites in breastfed infants with breast milk jaundice (BMJ) using microbiome–metabolomics. Breastfed newborns diagnosed BMJ those without (control group) were enrolled. Faecal samples collected from participants subjected high‐throughput sequencing of 16s rDNA V3 V4 regions flora metabolomics short‐chain fatty acids (SCFAs). Proteobacteria, Fimicutes Actinobacteria main bacteria at phylum level. Eshcerichia‐Shigella Enterobacteriacea...
// Wen-Hao Tang 1, * , Shan-Jie Zhou 2, Shi-De Song 3 Hui-Ying He 4 Han Wu 1 Zhe Zhang Yu-Zhuo Yang Hong-Liang 5 Jia-Ming Mao De-Feng Liu Lian-Ming Zhao Hao-Cheng Lin Kai Hong Lu-Lin Ma Xin-Jie Zhuang and Hui Jiang Department of Urology, Peking University Third Hospital, Beijing 100191, China 2 Reproductive Medicine Centre, International 102206, Rizhao People's Rizhao, Shandong 276826, Pathology, University, Health Science Center, Center for Medicine, Obstetrics Gynecology, These authors...
Abstract To improve the water solubility of anti‐human immunodeficiency virus (HIV) agent DB02 , an excellent non‐nucleoside reverse‐transcriptase inhibitor (NNRTI) obtained in our previous efforts, we designed and synthesized four phosphate derivatives based on molecular model with RT. Here, antiviral activity these was detected, leading to discovery compound P‐2 which possessed a superior potency lead against wild‐type HIV‐1 variety HIV‐resistant mutant viruses significantly. Furthermore,...
Testicular sperm aspiration (TESA) is widely used in the diagnosis and management of nonobstructive azoospermia. However, its ability for predicting microdissection testicular extraction azoospermia (NOA) patients with AZFc deletion remains uncertain. To investigate whether TESA affected retrieval rate (SRR) NOA deletion, a retrospective analysis clinical data who underwent (micro-TESE) was conducted. The effects age, volume, follicle-stimulating hormone (FSH) levels, luteinizing (LH)...