A5003522883- Neurogenetic and Muscular Disorders Research
- Amyotrophic Lateral Sclerosis Research
- Nerve injury and regeneration
- Single-cell and spatial transcriptomics
- RNA modifications and cancer
- Nerve Injury and Rehabilitation
- Congenital Anomalies and Fetal Surgery
- Neurogenesis and neuroplasticity mechanisms
- Muscle Physiology and Disorders
- Neuroinflammation and Neurodegeneration Mechanisms
- Neuroscience of respiration and sleep
- Adipose Tissue and Metabolism
- Parkinson's Disease Mechanisms and Treatments
- Epigenetics and DNA Methylation
- Cancer-related gene regulation
- RNA Research and Splicing
- Molecular Biology Techniques and Applications
- Botulinum Toxin and Related Neurological Disorders
- Cell Image Analysis Techniques
- RNA Interference and Gene Delivery
- T-cell and B-cell Immunology
- Biochemical Acid Research Studies
Karolinska Institutet
2015-2024
Stockholm University
2024
Natural and Medical Sciences Institute
2009-2010
University of Tübingen
2010
Abstract Laser capture microscopy (LCM) coupled with global transcriptome profiling could enable precise analyses of cell populations without the need for tissue dissociation, but has so far required relatively large numbers cells. Here we report a robust and highly efficient strategy LCM full-length mRNA-sequencing (LCM-seq) developed single-cell transcriptomics. Fixed cells are subjected to direct lysis RNA extraction, which both simplifies experimental procedures as well lowers technical...
Abstract The fatal disease amyotrophic lateral sclerosis (ALS) is characterized by the loss of somatic motor neurons leading to muscle wasting and paralysis. However, in oculomotor nucleus, controlling eye movement, are for unknown reasons spared. We found that insulin-like growth factor 2 (IGF-2) was maintained ALS thus could play a role resistance this disease. also showed IGF-1 receptor (IGF-1R), which mediates survival pathways upon IGF binding, highly expressed on extraocular endplate....
The lethal disease amyotrophic lateral sclerosis (ALS) is characterized by the loss of somatic motor neurons. However, not all neurons are equally vulnerable to disease; certain groups spared, including those in oculomotor nucleus controlling eye movement. reasons for this differential vulnerability remain unknown. Here we have identified a protein signature resistant and hypoglossal spinal mouse man health ALS with aim understanding neuron resistance. Several proteins implications...
Somatic motor neurons are selectively vulnerable in spinal muscular atrophy (SMA), which is caused by a deficiency of the ubiquitously expressed survival neuron protein. However, some groups, including oculomotor and trochlear (ocular), innervate eye muscles, for unknown reasons spared. To reveal mechanisms vulnerability resistance SMA, we investigate transcriptional dynamics discrete neuronal populations using laser capture microdissection coupled with RNA sequencing (LCM-seq). Using gene...
We examined whether skeletal muscle overexpression of PGC-1α1 or PGC-1α4 affected myokine secretion and neuromuscular junction (NMJ) formation. A microfluidic device was used to model endocrine signaling NMJ formation between primary mouse myoblast-derived myotubes embryonic stem cell-derived motor neurons. Differences in hydrostatic pressure allowed for fluidic isolation either cell type unidirectional the fluid phase. Myotubes were transduced overexpress PGC-1α4, quantified using a...
Abstract Amyotrophic lateral sclerosis (ALS) is characterized by the progressive loss of somatic motor neurons (MNs), which innervate skeletal muscles. However, certain MN groups including ocular MNs that regulate eye movement are relatively resilient to ALS. To reveal mechanisms differential susceptibility, we investigate transcriptional dynamics two vulnerable and populations in SOD1G93A ALS mice. Analysis differentially expressed genes (DEGs) shows each neuron type displays a largely...
Abstract Somatic motor neurons are selectively vulnerable in spinal muscular atrophy (SMA), a lethal disease caused by deficiency of the ubiquitously expressed survival neuron (SMN) protein. However, some brainstem groups, including oculomotor and trochlear (ocular), which innervate muscles around eyes, for unknown reasons spared. Here, using laser capture microdissection coupled with RNA sequencing (LCM-seq), we investigate transcriptional dynamics discrete neuronal populations health SMA...