A5080789148- Neurogenetic and Muscular Disorders Research
- Amyotrophic Lateral Sclerosis Research
- Stress Responses and Cortisol
- Neurogenesis and neuroplasticity mechanisms
- Receptor Mechanisms and Signaling
- Neuropeptides and Animal Physiology
- RNA modifications and cancer
- Extracellular vesicles in disease
- Muscle Physiology and Disorders
- Biofuel production and bioconversion
- Cancer-related gene regulation
- Process Optimization and Integration
- RNA Research and Splicing
- Catalysis for Biomass Conversion
- Developmental Biology and Gene Regulation
- Neuroinflammation and Neurodegeneration Mechanisms
- Epigenetics and DNA Methylation
- Pluripotent Stem Cells Research
Stockholm University
2022-2024
Abstract We performed single-nucleus RNA-sequencing on adult human spinal cord using a neuronal nuclei enrichment strategy. obtained transcriptomic profiles of >14,000 neurons, including small population motor neurons that shares similarities with mouse and can be subdivided into alpha gamma subtypes. sought to compare our results those from recent study by Yadav colleagues, which provides atlas the cord. While most both studies share similar features, differ substantially. reanalyzed...
Abstract Background Spinal muscular atrophy (SMA) is a form of motor neuron disease affecting primarily children characterised by the loss lower neurons (MNs). Breakdown neuromuscular junctions (NMJs) an early pathological event in SMA. However, not all are equally vulnerable, with some populations being lost while others remain intact at end-stage. A thorough understanding basis this selective vulnerability will give critical insight into factors which prohibit pathology certain and...
Major depressive disorder (MDD) is a serious disease and burden to patients, families society. Rodent experiments human studies suggest that several neuropeptide systems are involved in mood regulation. The aim of this study two-fold: (i) monitor, with qPCR, transcript levels the substance P/tachykinin (TAC), NPY CCK bulk samples from control suicide subjects, targeting five postmortem brain regions including locus coeruleus (LC); (ii) analyse expression family transcripts LC neurons...
Abstract Amyotrophic lateral sclerosis (ALS) is characterized by the progressive loss of somatic motor neurons (MNs), which innervate skeletal muscles. However, certain MN groups including ocular MNs that regulate eye movement are relatively resilient to ALS. To reveal mechanisms differential susceptibility, we investigate transcriptional dynamics two vulnerable and populations in SOD1G93A ALS mice. Analysis differentially expressed genes (DEGs) shows each neuron type displays a largely...
ABSTRACT BACKGROUND Major depressive disorder (MDD) is a serious disease and burden to patients, families society. Rodent experiments human studies suggest that several neuropeptide systems, including substance P(SP)/tachykinin, Y(NPY) their G protein-coupled receptors are involved in mood regulation. METHODS We assessed the transcript levels (qPCR) of SP/tachykinin NPY systems five regions from postmortem brains male female depressed subjects who committed suicide (DSS) controls:...
ABSTRACT Elongation of the posterior body axis is driven by multi-potent neuromesodermal progenitors (NMPs), which both self-renew and simultaneously generate neural tube, crest, presomitc mesoderm lineages at successive anterior (A-P) levels. The ensuing diversification these three NMP remarkably extensive, also essential for an immense range clinically important adult tissues. Here, we describe a human pluripotent stem cell protocol that successfully specifies authentic NMPs using cocktail...
Abstract Mutations in the RNA/DNA-binding proteins FUS and TDP-43 cause fatal disease amyotrophic lateral sclerosis (ALS). The precise mechanisms behind selective motor neuron degeneration remain unclear it is uncertain if ALS-causative mutations trigger death through shared or distinct pathogenic pathways. To address these two questions, we performed single-cell RNA sequencing across types derived from isogenic induced pluripotent stem cell lines, harbouring P525L, R495X, TARDBP M337V...