A5053300650- Adipose Tissue and Metabolism
- Adipokines, Inflammation, and Metabolic Diseases
- Cardiovascular Disease and Adiposity
- Mitochondrial Function and Pathology
- Diet and metabolism studies
- Cell death mechanisms and regulation
- Immune cells in cancer
- Lipid metabolism and biosynthesis
- Exercise and Physiological Responses
- Kruppel-like factors research
- Immune Cell Function and Interaction
- Epigenetics and DNA Methylation
- Fibroblast Growth Factor Research
- Metabolism, Diabetes, and Cancer
- Peroxisome Proliferator-Activated Receptors
- Nutrition, Genetics, and Disease
- Nuclear Structure and Function
- Neurogenetic and Muscular Disorders Research
- RNA regulation and disease
- Regulation of Appetite and Obesity
- IL-33, ST2, and ILC Pathways
- Immune Response and Inflammation
- NF-κB Signaling Pathways
- Amyotrophic Lateral Sclerosis Research
- PARP inhibition in cancer therapy
Stockholm University
2019-2024
University of Tübingen
2018-2020
German Center for Diabetes Research
2013-2020
Deutsches Diabetes-Zentrum e.V.
2017-2019
Heinrich Heine University Düsseldorf
2017-2019
Helmholtz Zentrum München
2013-2017
Universität Ulm
2009-2013
Ludwig-Maximilians-Universität München
2013
Center for Environmental Health
2013
ETH Zurich
2013
Brown adipose tissue (BAT) is a heater organ that expresses thermogenic uncoupling protein 1 (UCP1) to maintain high body temperatures during cold stress. BAT thermogenesis considered an overarching mammalian trait, but its evolutionary origin unknown. We show of marsupials, which diverged from eutherian mammals ~ 150 million years ago, nonthermogenic UCP1 variant governed by partial transcriptomic signature similar found in beige tissue. the reconstructed sequence common ancestor displayed...
Abnormal glucose metabolism is a central feature of disorders with increased rates cardiovascular disease. Low levels high-density lipoprotein (HDL) are key predictor for We used genetic mouse models HDL (apolipoprotein A-I transgenic [apoA-I tg]) and reduced (apoA-I-deficient ko]) to investigate whether modulates mitochondrial bioenergetics in skeletal muscle.
Mitochondrial dysfunction in white adipose tissue plays a key role the pathogenesis of type 2 diabetes. Emerging evidence specifically suggests that altered oxidative phosphorylation adipocytes may have relevant effect on systemic glucose homeostasis, requiring understanding adipocyte bioenergetics. We analyzed energetic flux an intact human cell model by plate-based respirometry and extracellular acidification. During differentiation, we discovered glycolytic ATP production was increasingly...
Brown adipose tissue (BAT) has been considered beneficial for metabolic health by participating in the regulation of glucose homoeostasis. The browning factors that improve uptake beyond normal levels are still unknown but is not affected UCP1 knockout mice. Here, we demonstrate human white adipocytes basal/resting improved solely elevating protein levels. Generating Simpson-Golabi-Behmel syndrome (SGBS) with a stable and overexpression UCP1, discovered overexpressing significantly 40%....
Abstract Among obese subjects, metabolically healthy (MHO) and unhealthy (MUHO) subjects exist, the latter being characterized by whole-body insulin resistance, hepatic steatosis, subclinical inflammation. Insulin resistance obesity are known to associate with alterations in mitochondrial density, morphology, function. Therefore, we assessed function human subcutaneous preadipocytes as well differentiated adipocytes derived from well-matched donors. Primary 4 insulin-resistant versus...
Tumor necrosis factor α (TNFα) and other members of the TNF family affect adipose tissue metabolism contribute to obesity-related inflammation tissue. Here, we sought identify effects TRAIL (TNF-related apoptosis-inducing ligand) on fat cell biology. TRAIL-receptor 2 (TRAIL-R2) its mouse homolog DR5 were regulated upon acute chronic energy imbalance in murine human inhibited insulin-stimulated glucose uptake de novo lipogenesis adipocytes. Interestingly, did not interfere with...
Tumor necrosis factor-α (TNFα) and other ligands of the TNF superfamily are potent regulators adipose tissue metabolism play a crucial role in obesity-induced inflammation tissue. Adipose expression levels TRAIL (TNF-related apoptosis-inducing ligand) its receptor were shown to be upregulated by overfeeding decreased fasting mice. In present study we aimed elucidate impact on adipogenesis. To this end, human Simpson-Golabi-Behmel syndrome (SGBS) preadipocytes as well stromal-vascular cells...
Obesity-associated WAT inflammation is characterized by the accumulation and local activation of macrophages (MΦs), recent data from mouse studies suggest that are modifiers adipocyte energy metabolism mitochondrial function. As dysfunction has been associated with obesity metabolic syndrome in humans, herein we aimed to delineate how human may affect white adipocytes. Human adipose tissue gene expression analysis for markers macrophage (CD11c, CD40, CD163, CD206, CD80, MCP1, TNFα)...
Abstract Expansion of adipose tissue mass by hypertrophy and hyperplasia is the hallmark obesity. An automated cDNA screen was established to identify secreted human proteins with an inhibitory effect on adipocyte differentiation and, thereby, a potential growth. A member TNF superfamily, TNF-like weak inducer apoptosis (TWEAK; superfamily 12) identified means high-throughput screening lipophilic dye Nile Red as inhibitor murine subsequently, also differentiation. TWEAK inhibited lipid...
Obesity is associated with an accumulation of macrophages in adipose tissue. This inflammation tissue a key event the pathogenesis several obesity-related disorders, particularly insulin resistance. Here, we summarized existing model systems that mimic situation inflamed vitro, most them being murine. Importantly, introduce our newly established human system which combines THP-1 monocytic cell line and preadipocyte strain Simpson-Golabi-Behmel syndrome (SGBS). cells, originate from acute...
Aims/hypothesis The excessive accumulation of adipose tissue in the obese state is linked to an altered secretion profile adipocytes, chronic low-grade inflammation and metabolic complications. RBP4 has been implicated these alterations, especially insulin resistance. aim present study was determine if a local inflammatory micro-environment regulates expression secretion. Methods Human SGBS primary adipocytes cultured with conditioned media from human THP-1 macrophages were used as vitro...
Although the prevalence of obesity and its associated metabolic disorders is increasing in both sexes, clinical phenotype differs between men women, highlighting need for individual treatment options. Mitochondrial dysfunction various tissues, including white adipose tissue (WAT), has been accepted as a key factor obesity-associated comorbidities such diabetes. Given higher expression mitochondria-related genes WAT we hypothesized that gender differences bioenergetic profile (pre-)...
Background: Tissue-resident macrophages have mixed developmental origins. They derive in variable extent from yolk sac (YS) hematopoiesis during embryonic development. Bone marrow (BM) hematopoietic progenitors give rise to tissue postnatal life, and their contribution increases upon organ injury. Since the phenotype functions of are modulated by residence, impact origin paths has remained incompletely understood. Methods: In order decipher cell-intrinsic macrophage programs, we immortalized...