A5089156329- Multiple Myeloma Research and Treatments
- Immunotherapy and Immune Responses
- Glycosylation and Glycoproteins Research
- Monoclonal and Polyclonal Antibodies Research
- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Chemokine receptors and signaling
- Polymer Nanocomposites and Properties
- CAR-T cell therapy research
- Chronic Lymphocytic Leukemia Research
- Cancer Treatment and Pharmacology
- Amyloidosis: Diagnosis, Treatment, Outcomes
- Polymer Science and PVC
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Education, Psychology, and Social Research
- Cancer therapeutics and mechanisms
- Peptidase Inhibition and Analysis
- Central Venous Catheters and Hemodialysis
- Platelet Disorders and Treatments
- Polymer crystallization and properties
- Hepatitis B Virus Studies
- RNA Interference and Gene Delivery
- Dialysis and Renal Disease Management
- Taxation and Legal Issues
- Polymer Nanocomposite Synthesis and Irradiation
General University Hospital in Prague
2018-2021
Charles University
2018-2021
Czech Academy of Sciences, Institute of Microbiology
2021
University Hospital Brno
2003-2012
Masaryk University
2003-2012
Institut National des Sciences Appliquées de Lyon
2010
Czech Academy of Sciences, Institute of Macromolecular Chemistry
2010
Tomas Bata University in Zlín
2005-2010
Czech Academy of Sciences, Institute of Biophysics
2003
The European Myeloma Network (EMN) organized two flow cytometry workshops. first aimed to identify specific indications for in patients with monoclonal gammopathies, and consensus technical approaches through a questionnaire-based review of current practice participating laboratories. second resolve outstanding issues develop approach analysis plasma cells. primary clinical applications identified were: differential diagnosis neoplastic cell disorders from reactive plasmacytosis; identifying...
<b><i>Background/Aims:</i></b> High rates of cognitive impairment (CI) are an alarming problem in patients undergoing chronic hemodialysis (HD). Its pathophysiology remains unclear and there indications that brain ischemia might be one the key causes. Cerebral tissue oxygenation, as measured by near-infrared spectroscopy, is known to decreased HD patients. However, it unknown whether CI associated or not with lower cerebral oxygenation these The primary aim our study...
In a phase II clinical study, pretreated multiple myeloma patients with relapsing or stable disease received autologous anticancer vaccine containing dendritic cells loaded Id-protein.Patients total of 6 doses intradermally in monthly intervals.No responses were observed.During the follow-up median 33.1 months (range: 11-43 months), remained 7/11 (64%) patients.Immune measured by ELISpot noted 3/11 (27%) and DTH skin test for Id-protein was positive 8/11 (73%) patients; out those, 1/11 (9%)...
AimsPatients on chronic haemodialysis have a wide range of changes in cardiac function and structure, including left ventricular hypertrophy, dilation diastolic dysfunction or pulmonary hypertension. All these were linked to increased mortality previous studies. High-flow arteriovenous fistulas (AVF) are supposed be factor contributing their development. This study investigated the early effect surgical AVF blood flow (Qa) reduction patients with without heart failure changes.Methods...
Dendritic cells are able to induce anti-tumor immune responses by presenting tumor-specific antigens T-lymphocytes. Various tumor-associated have been studied in multiple myeloma an effort find a strong antigen capable of generating clinically meaningful vaccinated patients. The aim our study was generate myeloma-specific cytotoxic T lymphocytes vitro using dendritic loaded with peptide or apoptotic bodies. Peripheral blood mononuclear from HLA-A2+ healthy donors were used for isolation and...
Abstract In this paper poly(vinyl chloride)/clay nanocomposites were prepared by melt intercalation using a single screw extruder. Problems with thermal stability of these during compounding largely eliminated pre‐treatment the organoclay plasticizer (dioctyl phthalate), which created barrier between polymer and quaternary amine. These nanocomposite materials analyzed respect to their morphology. The intercalation, exfoliation, nano‐phase dispersion orientation investigated Transmission...
Flow cytometry is a useful tool for the analysis of plasma cells in monoclonal gammopathies. The aim this study was to find possibilities and limits multicolour flow diagnostics gammopathy undetermined significance (MGUS) multiple myeloma (MM) identify parameters that could be used differentiate between these two disorders. Surface markers CD38 CD138 were identification cells, CD19 CD56 further distinguished normal abnormal respectively. percentage circulating peripheral blood lower MGUS...
Significance Photosynthesis uses chlorophylls to utilize solar energy. In oxygenic phototrophs, formation of the isocyclic fifth ring chlorophyll, responsible for its green color, is catalyzed by AcsF/CycI and auxiliary protein Ycf54. Removal Ycf54 causes severe chlorophyll deficiency impaired photoautotrophic growth. We analyzed laboratory-evolved suppressor mutants a Ycf54-less strain cyanobacterium Synechocystis where biosynthesis phototrophy were restored. A single point mutation in CycI...
Background: Creation of vascular access leads to considerable local haemodynamic changes with decreased hand perfusion. Distal limb tissues then represent a model ischaemia effect on muscles. The aim our study was investigate how the presence influences muscle strength in end-stage renal disease patients. Methods: We included 52 chronically haemodialysed patients upper without clinical signs ischaemia. Muscle evaluated by dynamometry. Finger pressure measured second and fourth fingers...
Brain tissue oxygenation (rSO(2)) measured by near-infrared spectroscopy (NIRS) is lower in hemodialysis patients than the healthy population and associated with cognitive dysfunction. The involved mechanisms are not known. We conducted this study to identify factors that influence rSO2 values end-stage renal disease (ESRD) describe changes during hemodialysis. included a cohort of ESRD hemodialyzed our institution. recorded using INVOS 5100C oximetry system (Medtronic, Essex, U.K.) analyzed...
Anticancer immunotherapy using dendritic cell-based vaccines is a strategy aimed at the induction and maintenance of immune responses against cancer cells. Clinical applications cells (DCs) require stringent adherence to Good Manufacturing Practice (GMP) methods rigorous standardization DC-based vaccine preparation. Recently, closed systems for DC culture have been developed with goal minimize risk contamination. Here, we compare yield, immunophenotype, functional properties DCs generated in...
Background: Decreased cerebral perfusion and oxygenation are common in hemodialysis patients. Magnitude of the arteriovenous fistula involvement this phenomenon is not known. The aim study was to investigate effect that a short-term flow interruption has on review suggest possible explanations. Methods: In 19 patients, basic laboratory clinical data were obtained volume measured by ultrasonography. Baseline regional oxygen saturation (rSO 2 ) near-infrared spectroscopy. Manual pressure then...
Polyvinylchloride compositions have been prepared by the melt intercalation method using a single screw extruder. Different types of nanofiller based on natural sodium montmorillonite were tested. In particular, intercalating agents containing amine groups combined with co-intercalating agent (low or high molecular weight plasticiser) examined to determine which is most suitable not only for producing largest basal spacing and subsequently best exfoliation clay tactoids in polymer matrix,...