Tannic acid, a rich of natural and process-derived phenolic compound, has been shown to be an effective antagonist against viruses bacteria. In this study, we determined the antimicrobial activity mechanisms tannic acid Staphylococcus aureus with emphasis on inhibiting effect biofilm formation. Based results time-kill assay, binding ability lysozyme susceptibility assay transmission electron microscope, tentatively speculated that peptidoglycan might target process destroy integrity cell...

Abstract Background Klebsiella pneumoniae -induced pyogenic liver abscess (KP-PLA) has emerged as a life-threatening disease worldwide. However, to date, limited number of scholars have attempted systematically elucidate the characteristics KP-PLA. The aim present study was analyze clinical, microbiological, and molecular epidemiological KP-PLA patients in Southeastern China. Methods cases from tertiary teaching hospital China January 2016 December 2017 were systemically studied elucidated...

Carbapenem-resistant, hypervirulent Klebsiella pneumoniae (CR-hvKP) has recently emerged as a significant threat to public health. In this study, 29 K. isolates were isolated from eight patients admitted the intensive care unit (ICU) of comprehensive teaching hospital located in China March 2017 January 2018. Clinical information was basis for further analyses including antimicrobial susceptibility tests, identification antibiotic resistance and virulence gene determinants, multilocus...

The rise in diversity of antimicrobial resistance phenotypes seen Klebsiella pneumoniae is becoming a serious antibiotic management problem. We sought to investigate the molecular characteristics and clinical implications extensively drug-resistant (XDR) K. isolated from different nosocomial bloodstream infections (BSIs) patients July 2013 November 2015. Even combination treatment, meropenem did not protect against mortality BSIs (P = 0.015). In contrast, tigecycline with other agents...

Introduction. Autoinducer-2 (AI-2) quorum sensing is a bacterial communication system that responds to cell density. The requires luxS activity produce AI-2, which can regulate gene expression and processes such as biofilm formation.Aim. To investigate the role of in formation nosocomial pathogen Klebsiella pneumoniae.Methodology. A ΔluxS deletion was made K. pneumoniae KP563, an extensively drug-resistant isolate. AI-2 production assessed wild-type strains grown media supplemented with...

Social hygiene is seriously threatened by the rise in colistin (COL) resistance against Gram-negative bacteria (GNB). With to last-line antibiotics such as COL becoming more common, it imperative identify alternative treatment options. In our work, we sought determine if plus kaempferol (KP) present synergistic effects on antibacterial and antibiofilm activities colistin-resistant (Col-R) GNB vivo vitro. Twenty-four Col-R were collected experimental strains. The activity of KP was evaluated...

The aim of the present study was to evaluate predation efficacy Bdellovibrio bacteriovorus on multidrug-resistant (MDR) or extensive drug resistant (XDR) gram-negative pathogens and their corresponding biofilms. In this study, we examined ability B. prey MDR XDR clinical bacteria, including Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Acinetobacter baumannii. Results showed that able all planktonic cultures, among which most efficient observed for drug-resistant E. with a...

The goal was to investigate the mechanisms of colistin resistance and heteroresistance in Pseudomonas aeruginosa clinical isolates. Colistin determined by broth microdilution method. evaluated population analysis profiling. Time-kill assays were also conducted. PCR sequencing performed detect genes among (hetero)resistant isolates, quantitative real-time determine their expression levels.

The emergence and spread of carbapenem-resistant Escherichia coli (E. coli) pose a serious threat to human health worldwide. This study aimed investigate the molecular mechanisms underlying carbapenem resistance their prevalence among E. in China.A collection 5796 clinical isolates were collected from First Affiliated Hospital Wenzhou Medical University 2002 2017. Sensitivity antibiotics was determined using agar dilution method. detection carbapenemases production resistance-associated...

Abstract Background Klebsiella pneumoniae is considered the most clinically relevant species of Enterobacteriaceae, known to cause severe infections including liver abscesses. To best our knowledge, a large proportion iron in human body accumulated and stored liver. We hypothesize that increased availability an important factor driving abscess formation we therefore aim understand effects on K. causing Results All tested clinical isolates, those isolated from abscesses other abdominal...

Abstract Background The emergence of carbapenem-resistant and colistin-resistant ECC pose a huge challenge to infection control. purpose this study was clarify the mechanism carbapenems colistin co-resistance in Enterobacter cloacae Complex (ECC) strains. Results This showed that mechanisms carbapenem resistance are: 1. Generating carbapenemase (7 19); 2. production AmpC or ESBLs combined with decreased expression out membrane protein (12 19). hsp60 sequence analysis suggested 10 19 strains...

Abstract Background Bloodstream infection (BSI) caused by multidrug-resistant Acinetobacter baumannii (MDR-AB) has been increasingly observed among hospitalized patients. The following study analyzed the epidemiology and microbiological characteristics of MDR-AB, as well clinical features, antimicrobial treatments, outcomes in patients over a six years period China. Methods This retrospective was conducted large tertiary hospital China between January 2013 December 2018. data all consecutive...

The spread of multidrug-resistant (MDR) Gram-negative bacteria (GNB) has led to serious public health problems worldwide. Colistin, as a "last resort" for the treatment MDR bacterial infections, been used significantly in recent years and continuous emergence colistin-resistant strains. In this study, we aimed investigate synergistic effect on antimicrobial antibiofilm activities colistin/furanone C-30 combination against GNB vitro vivo. According resistance profiles, most strains collected...

Colistin-resistant (Col-R) bacteria are increasing sharply, which poses a serious threat to public health. Thymol is phenolic compound used for its wide-spectrum antimicrobial activity, while the combination of nontraditional drugs restore colistin activity an attractive strategy treat infections caused by these pathogens. This study showed that thymol could play synergistic role with against Gram-negative (GNB), including nonfermenting and Enterobacteriaceae. According resistance profiles,...

Abstract Background We aimed to determine the evolutionary pathways of rifampicin resistance in Staphylococcus aureus , and impact mutations rpoB gene on fitness. Methods Three clinical strains one reference strain were used select for rifampicin-resistant S. variants. The responsible all selected isolates vitro investigated by polymerase chain reaction (PCR) DNA sequencing. To compare fitness cost against their corresponding original isolates, we performed bacterial growth curve assays,...

Abstract Background The study aimed to elucidate the species taxonomy, clinical manifestations, virulence gene profiles and antimicrobial susceptibilities of Aeromonas strains isolated from life-threatening bacteremia in southeastern China. Methods Clinical samples causing were a teaching hospital Wenzhou 2013 2018 retrospective cohort was performed. identified at level by housekeeping gyrB . Virulence drug resistance-associated genes screened polymerase chain reaction (PCR) susceptibility...

The emergence and spread of carbapenem-resistant Klebsiella pneumoniae (CRKP) pose a threat to public health. Antimicrobial peptides provide new treatment option for CRKP infections.We studied antibacterial activities WAM-1 against in vitro vivo explored its possible mechanism. We verified safety factors affecting effect. Furthermore, anti-inflammatory effects were investigated.We selected eight carbapenem-susceptible K. explore the activity by broth microdilution (BMD). mechanism was...

The global emergence and spread of multi-drug resistant (MDR) strains is becoming increasingly worrisome due to the overuse broad-spectrum antibiotics. Colistin, last resort for treating MDR infections, has once again returned clinician’s choice. However, with widespread use colistin, colistin-resistant gram-negative bacteria (GNB) have subsequently emerged, including Pseudomonas aeruginosa (COL-R PA). Therefore, available solutions are urgently needed respond this situation. Here, we...

Klebsiella pneumoniae (K. pneumoniae) is one of the most common causes bacterial meningitis worldwide. The purpose this study was to investigate clinical and microbiological characteristics K. meningitis, as well association antimicrobial resistance, virulence, patient prognosis. data patients with from 2014 2020 in a tertiary teaching hospital were retrospectively evaluated. Antimicrobial susceptibility profiles performed by agar dilution method broth microdilution method. isolates detected...

Carbapenem-resistant gram-negative bacteria (CR-GNB) pose a significant threat to public health and require immediate attention. The development of novel antibacterial agents against CR-GNB has become an urgent priority, nanomaterials offer promising solutions due their unique properties. This study introduces 5-amino-2-mercaptobenzimidazole (5-A-2MBI) functionalized gold nanoparticles (5-A-2MBI_Au NPs) evaluates activity CR-GNB. 5-A-2MBI_Au NPs was synthesized using one-pot method. Its...