Summary Multi‐omics approach was adopted to investigate the modulation of bacterial microbiota and metabolome as well their interactions in whole crop corn ensiling systems by inoculating homofermentative Lactobacillus plantarum or heterofermentative buchneri . Inoculations two different inoculants resulted substantial differences microbial community metabolic composition dynamics ensiled corn. Inoculants also altered correlations manners, various keystone species were identified silages...

Significance We provide insights into the sequence–conformation–property relationship that is central to mechanical properties of protein elastomers. find a high content glycine residue alone, not including proline, sufficient for achieving near-perfect resilience. The proline may be associated with metastability random coils. Also, Raman spectroscopy, as potent tool investigating conformation–property relationship, gives rise direct correlation between semiquantitative features and...

Field-effect transistors (FETs), when functionalized with proper biorecognition elements (such as antibodies or enzymes), represent a unique platform for real-time, specific, label-free transduction of biochemical signals. However, direct immobilization molecules on FETs imposes limitations reprogrammability, sensor regeneration, and robust device handling. Here we demonstrate modularized design FET biosensors separate transducer modules, which are capable reversible assembly disassembly. In...

<title>Abstract</title> <bold>Background:</bold>Spinal cord cysticercosis, a rare subtype of neurocysticercosis (NCC), primarily affects the subarachnoid space. The optimal treatment approach remains uncertain, with both pharmacological and surgical strategies being employed. Spinal stimulation (SCS) has been established as safe effective therapy for chronic pain,we present case neuropathic pain caused by spinal successfully managed SCS. <bold>Case presentation:</bold>A 69-year-old female...

Agriculture is at the pivot point between anthroposphere, biosphere, and atmosphere. Innovative solutions are needed to reduce agricultural emissions improve sustainability. Microalgae animal feed could be such a solution. This study aimed evaluate effects of 10 freshwater microalgae: Auxenochlorella protothecoides, Chlamydomonas pulvinate, Chlorella luteoviridis, variabilis, Euglena mutabilis, Parachlorella kessleri, Stichococcus bacillaris, Tetradesmus acuminatus, obliquus, Tetraselmis...

Probing and facilitating microbial extracellular electron transfer through nanotechnology enabled platforms are transforming bioenergetic, bioelectronic, other related research areas.

Shaoyao Gancao decoction (SGD), a well-known Chinese herbal formula, has been used to treat liver injury for long time. In this study, chemical profiles of SGD were identified using ultra high-performance liquid chromatography combined with Fourier transform ion cyclotron resonance mass spectrometry (UHPLC-FT-ICR-MS/MS). Liquid was performed on C18 column (150 mm × 2.1 mm, 1.8 μm); the mobile phase comprised 0.1% formic acid (A) and acetonitrile (B). We then characterized 73 compounds;...

Bottom-up engineering approaches are transforming biological wastewater treatment.

The effects of two strains class IIa bacteriocin-producing lactic acid bacteria, Lactobacillus delbrueckii F17 and plantarum (BNCC 336943), or a non-bacteriocin MTD/1 (NCIMB 40027), on fermentation quality, microbial counts, aerobic stability alfalfa silage were investigated. Alfalfa was harvested at the initial flowering stage, wilted to dry matter concentration approximately 32%, chopped 1 2 cm length. Chopped samples treated with nothing (control, CON), (F17), 336943) (LPB), 40027) (LPN),...

An increasing number of K‐12 school teachers have been using handheld, or palmtop, computers in the classroom as an integral means facilitating education due to flexibility, mobility, interactive learning capability, and comparatively inexpensive cost. This study involved two experiments handheld computers: (a) a comparison process English spelling fourth grade students who did not use computers, (b) between test results fifth students’ dividing fractions paper‐and‐pencil. The revealed that...

Extracellular electron transfer (EET) from electrochemically active bacteria (EAB) plays a critical role in renewable bioelectricity harvesting through microbial fuel cells (MFC). Comprehensive interpretation and interrogation of EET mechanisms can provide valuable information to enhance MFC performance, which however are still restricted by the intrinsic complexity natural biofilm. Here, we design core/shell EAB-encapsulating cables as one-dimensional model system facilitate studies, where...

Riverine ecosystems are linked to their watersheds, and both land use physiographic environmental conditions influence nutrient dynamics water quality. We assessed aquatic nutrients relationship with at multiple spatial scales in the Brazos River watershed (Texas, USA) examine interactions between physiography combined influences on riverine dynamics. Patterns were highly correlated, but gradients explained ∼2× more of variability concentrations than (25 12%, respectively). The response...

Electron transport in biological and inorganic systems is mediated through distinct mechanisms pathways. Their fundamental mismatch structural thermodynamic properties has imposed a significant challenge on the effective coupling at biotic/abiotic interface, which central to design development of bioelectronic devices their translation toward various engineering applications. Using electrochemically active bacteria, such as G. sulfurreducens, model system, here we report bottom-up,...

Ultrasmall Ru nanoparticles is expected as a potential alternative to Pt for efficient hydrazine oxidation (HzOR). However, preparation of ultrasmall and well-distributed usually suffered from the steps modification supports, coordination, reduction with strong reducing reagents (e.g., NaBH4) or pyrolysis, imposing complexity. Based on self-reducibility C–OH group physical adsorption ability commercial Ketjen black (KB), we developed an efficient, stable robust Ru-based electrocatalyst...

Living composites comprising of both biotic and abiotic modules are shifting the paradigm materials science, yet challenges remain in effectively converging their distinctive structural functional attributes. Here we present a bottom-up hybridization strategy to construct functionally coherent, electrochemically active biohybrids with optimal mass/charge transport, mechanical integrity, biocatalytic performance. This biohybrid can overcome several key limitations traditional biocarrier...

In the inaugural year of 14th Five-Year Plan, General Secretary Xi Jinping profoundly emphasized that promoting rural revitalization development is a comprehensive and historical task essential for building modern socialist country. Education cornerstone plays pioneering role in this process. It crucial to consolidate expand achievements educational poverty alleviation seamlessly connect them with revitalization. Empowering through education both responsibility mission education. This paper...