A5101627830- SARS-CoV-2 detection and testing
- SARS-CoV-2 and COVID-19 Research
- Tea Polyphenols and Effects
- Spectroscopy Techniques in Biomedical and Chemical Research
- Plant Gene Expression Analysis
- Plant biochemistry and biosynthesis
- HIV Research and Treatment
- Food Quality and Safety Studies
- HIV/AIDS drug development and treatment
- Monoclonal and Polyclonal Antibodies Research
- Phytochemicals and Antioxidant Activities
- Microbial Metabolism and Applications
- Fungal Biology and Applications
- Glycosylation and Glycoproteins Research
- Plant and Fungal Interactions Research
- Photosynthetic Processes and Mechanisms
- Heat shock proteins research
- Molecular Biology Techniques and Applications
- Genomics and Phylogenetic Studies
- Bacteriophages and microbial interactions
- Metabolomics and Mass Spectrometry Studies
- Hepatitis C virus research
- Tannin, Tannase and Anticancer Activities
- Plant Molecular Biology Research
- Protein purification and stability
Northwest A&F University
2016-2024
Tea leaves contain abundant flavan-3-ols, which include dihydroxylated and trihydroxylated catechins. Flavonoid 3'-hydroxylase (F3'H: EC 1.14.13.21) is one of the enzymes in establishment hydroxylation pattern. A gene encoding F3'H, designated as CsF3'H, was isolated from Camellia sinensis with a homology-based cloning technique deposited GenBank (GenBank ID: KT180309). Bioinformatic analysis revealed that CsF3'H highly homologous characterized F3'Hs other plant species. Four conserved...
Catechins are critical constituents for the sensory quality and health-promoting benefits of tea. Cytochrome P450 monooxygenases required catechin biosynthesis dependent on NADPH-cytochrome reductases (CPRs) to provide reducing equivalents their activities. However, CPRs have not been identified in tea, relationship accumulation also remains unknown. Thus, three CsCPR genes were this study, all which had five CPR-related conserved domains targeted endoplasmic reticulum. These recombinant...
Tea contains a variety of flavone C-glycosides, which are important compounds that distinguish tea cultivars and categories. However, the biosynthesis pathway C-glycosides in plant remains unknown, key enzymes involved have not been characterized. In this study, liquid chromatography–mass spectrometry method to determine 9 was developed, accumulation patterns plants were examined first. Then, an entry enzyme CsF2H for C-glycoside identified, had four cytochrome P450-specific conserved motifs...