A5010074093- Regulation of Appetite and Obesity
- Aquaculture Nutrition and Growth
- Reproductive biology and impacts on aquatic species
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Biochemical Analysis and Sensing Techniques
- Adipokines, Inflammation, and Metabolic Diseases
- Zebrafish Biomedical Research Applications
- Epigenetics and DNA Methylation
- Adipose Tissue and Metabolism
- Physiological and biochemical adaptations
- Estrogen and related hormone effects
- Immune responses and vaccinations
- Aquaculture disease management and microbiota
- Fish Ecology and Management Studies
- Cellular transport and secretion
- Lipid Membrane Structure and Behavior
- Growth Hormone and Insulin-like Growth Factors
- Genetic and Kidney Cyst Diseases
- Neurobiology and Insect Physiology Research
- melanin and skin pigmentation
- Renal and related cancers
North Carolina State University
2013-2024
National Center for Cool and Cold Water Aquaculture
2021-2022
United States Department of Agriculture
2021-2022
Duke University
2013-2020
Significance Inflammatory bowel diseases (IBD), including Crohn’s disease and ulcerative colitis, are intestinal disorders of poorly understood origin associated with significant morbidity mortality. A crucial factor IBD onset is the presence elevated levels proinflammatory cytokine tumor necrosis (TNF) in intestine, signified by use anti-TNF therapy to treat patients disease. Despite its pathogenic relevance, mechanisms regulating TNF expression remain largely unknown. Here, we show that...
Environmental sex-determination (ESD) is the phenomenon by which environmental factors regulate sex-determination, typically occurring during a critical period of early development. Southern flounder (Paralichthys lethostigma) exhibit temperature-dependent that appears to be restricted presumed XX female genotype with extremes temperature, both high and low, skewing sex ratios toward males. In order evaluate other may influence we investigated background color cortisol on in southern...
Southern flounder (Paralichthys lethostigma) exhibit environmental sex determination (ESD), where factors can influence phenotypic during early juvenile development but only in the presumed XX female genotype. Warm and cold temperatures masculinize fish with mid-range conditions producing at most 50% females. Due to sexually dimorphic growth, southern fisheries are dependent upon larger Wild populations could be risk of masculinization from ESD due globally increasing water temperatures. We...
Forward genetic approaches in zebrafish have provided invaluable information about developmental processes. However, the relative difficulty of mapping and isolating mutations has limited number new screens. Recent improvements annotation genome coupled to a reduction sequencing costs prompted development whole RNA for gene discovery. Here we describe exome (WES) approach that allows rapid cost-effective identification mutations. We used our WES methodology isolate four cause kidney cysts;...
Epithelial cell physiology critically depends on the asymmetric distribution of channels and transporters. However, mechanisms targeting membrane proteins to apical surface are still poorly understood. Here, we performed a visual forward genetic screen in zebrafish intestine identified mutants with defective proteins. One these mutants, affecting vacuolar H+-ATPase gene atp6ap1b, revealed specific requirements for luminal acidification apical, but not basolateral, protein sorting transport....
Leptin is a pleiotropic hormone known for regulating appetite and metabolism. To characterize the role of leptin signaling in rainbow trout, we used CRISPR/Cas9 genome editing to disrupt receptor (LepR) genes, lepra1 lepra2. We compared wildtype (WT) mutant fish that were either fed satiation or feed deprived six weeks. The LepR mutants exhibited hyperphagic phenotype, which led heavier body weight, faster specific growth rate, increased viscero- hepatosomatic indices, greater condition...
Leptin is a cytokine that regulates appetite and energy expenditure, where in fishes it primarily produced the liver acts to mobilize carbohydrates. Most have only one leptin receptor (LepR/LepRA1), however, paralogs recently been documented few species. Here we reveal second (LepRA2) rainbow trout 77% similar LepRA1. Phylogenetic analyses show salmonid specific genome duplication event as probable origin of LepR trout. Tissues distributions showed tissue expression these receptors, with...
Leptin, insulin, and glucagon are involved in regulating glycaemia vertebrates play a role the progression of obesity type 2 diabetes. While mammals possess an ‘adipoinsular axis’ whereby insulin stimulates leptin release from adipocytes turn feeds back on pancreas to inhibit further secretion, evidence such axis non-mammalian is unknown. We investigated interactions between these glycaemic hormones provide for leptin–insulin teleost fish, tilapia. In first study, we exposed hepatocytes...
Abstract California halibut ( Paralichthys californicus ) is a candidate species for aquaculture and stock enhancement. These applications rely on sex control, either to maximize the production of faster growing females or match ratios in wild. Other paralichthids exhibit temperature‐dependent determination (TSD), but presence pattern TSD not well defined halibut. Juvenile were cultured at three distinct temperatures (15°C, 19°C, 23°C) through developmental period presumed be thermosensitive...