A5083185038- interferon and immune responses
- Immune Response and Inflammation
- Galectins and Cancer Biology
- Inflammasome and immune disorders
- Zebrafish Biomedical Research Applications
- Immune responses and vaccinations
- Genetic Mapping and Diversity in Plants and Animals
- Immune cells in cancer
- Epigenetics and DNA Methylation
- Mycobacterium research and diagnosis
- Peripheral Neuropathies and Disorders
- Genetics and Neurodevelopmental Disorders
- Aquaculture disease management and microbiota
- Neuroinflammation and Neurodegeneration Mechanisms
- Bone and Dental Protein Studies
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Inflammation biomarkers and pathways
- T-cell and B-cell Immunology
- Hippo pathway signaling and YAP/TAZ
- Fungal Infections and Studies
- Systemic Lupus Erythematosus Research
- Sphingolipid Metabolism and Signaling
- Genetic and phenotypic traits in livestock
- Cellular transport and secretion
- Sleep and Wakefulness Research
Yale University
2013-2025
Duke University
2018-2022
Memorial Sloan Kettering Cancer Center
2015
University of London
2012
London School of Hygiene & Tropical Medicine
2012
Jackson Laboratory
2009
Novartis (Japan)
2009
University of Tsukuba
2009
Significance Inflammatory bowel diseases (IBD), including Crohn’s disease and ulcerative colitis, are intestinal disorders of poorly understood origin associated with significant morbidity mortality. A crucial factor IBD onset is the presence elevated levels proinflammatory cytokine tumor necrosis (TNF) in intestine, signified by use anti-TNF therapy to treat patients disease. Despite its pathogenic relevance, mechanisms regulating TNF expression remain largely unknown. Here, we show that...
Acquired immunity in vertebrates maintains polymorphisms endemic pathogens, leading to identifiable signatures of balancing selection. To comprehensively survey for genes under such selection the human malaria parasite Plasmodium falciparum, we generated paired-end short-read sequences parasites clinical isolates from an Gambian population, which were mapped 3D7 strain reference genome yield high-quality genome-wide coding sequence data 65 isolates. A minority did not map reliably, including...
Pulmonary fungal infections generate heterogenous alveolar macrophage subpopulations.
Inflammasomes are a class of innate immune signaling platforms that activate in response to an array cellular damage and pathogens. promote inflammation under many circumstances enhance immunity against pathogens inflammatory responses through their effector cytokines, IL-1β IL-18. Multiple sclerosis its animal model, experimental autoimmune encephalomyelitis (EAE), conditions influenced by inflammasomes. Despite work investigating inflammasomes during EAE, little remains known concerning...
Multiple sclerosis (MS) is a complex genetically mediated autoimmune disease of the central nervous system where anti-CD20-mediated B cell depletion remarkably effective in treatment early disease. While previous studies investigated effect on select immune subsets using flow cytometry-based methods, therapeutic impact patient landscape unknown. In this study, we explored how depleting therapies modulate single-cell RNA sequencing (scRNAseq). We demonstrate that leads to type-specific...
The genetic basis of hypertension is well established, yet very few genes that cause common forms are known. Quantitative trait locus (QTL) analyses in rodent models can guide the search for human genes, but excellent resources mice have been underused this regard. To address issue, we surveyed blood pressure variation from 37 inbred strains and generated 2577 8 intercross populations to perform QTL pressure. We identified 14 these populations, including > or =7 regions mouse genome not...
Neutrophils are critical as the first-line defense against fungal pathogens. Yet, previous studies indicate that neutrophil function is complex during Cryptococcus neoformans (Cn) infection. To better understand role of neutrophils in acute pulmonary cryptococcosis, we analyzed heterogeneity by single-cell transcriptional analysis immune cells lung Cn -infected mice from a published dataset. We identified reference-based annotation and two distinct subsets generated infection: A subset with...
Abstract Multiple sclerosis (MS) is a complex genetically mediated autoimmune disease of the central nervous system where anti-CD20-mediated B cell depletion remarkably effective in treatment early disease. While previous studies investigated effect on select immune subsets using flow cytometry-based methods, therapeutic impact patient landscape unknown. In this study, we explored how therapy-driven “ vivo perturbation ” modulates diverse by measuring transcriptomic granularity with...
Osteopontin (OPN) has been considered a potential biomarker of graft-versus-host disease (GVHD). However, the function OPN in GVHD is still elusive. Using mouse model acute (aGVHD), we report that generated by CD4+ T cells sufficient to exert beneficial effect controlling aGVHD through limiting gastrointestinal pathology, major target organ aGVHD. cell-derived works on CD44 expressed intestinal epithelial (IECs) and abates cell death IECs. also modulates gut microbiota with enhanced...
Environmental factors can stably perturb the epigenome of exposed individuals and even that their offspring, but pleiotropic effects these have posed a challenge for understanding determinants mitotic or transgenerational inheritance epigenetic perturbation. To tackle this problem, we manipulated states various target genes using tetracycline-dependent transcription factor. Remarkably, transient manipulation at appropriate times during embryogenesis led to aberrant modifications in ensuing...
Abstract Dectin-1 is known to drive proinflammatory cytokine production by macrophages and dendritic cells which promotes Th17 CD4+ T cell responses in the setting of fungal infection. However, role signaling neutrophils its impact on not well understood. In this study, we found that stimulated with a agonist diminish viability rapid reactive oxygen species (ROS)-dependent manner. Furthermore, promoted neutrophil PD-L1 expression via Syk Card9 signaling, along other immune-checkpoint factors...
ABSTRACT Inflammasomes are a class of innate immune signaling platforms that activate in response to an array cellular damage and pathogens. promote inflammation under many circumstances enhance immunity against pathogens inflammatory responses through their effector cytokines, IL-1β IL-18. Multiple sclerosis its animal model, experimental autoimmune encephalomyelitis (EAE), such conditions influenced by inflammasomes. Despite work investigating inflammasomes during EAE, little remains known...
ABSTRACT Pathologic roles for innate immunity in neurologic disorders are well-described, but protective aspects of the immune response less understood. Dectin-1, a C-type lectin receptor (CLR), is largely known to induce inflammation. However, we found that Dectin-1 experimental autoimmune encephalomyelitis (EAE), while its canonical signaling mediator, Card9, promotes disease. Notably, does not respond heat-killed Mycobacteria , an adjuvant EAE. Myeloid cells mediate function EAE and...
Abstract Innate immunity mediates both damage and repair of the central nervous system (CNS) in neurologic disorders. Although pathologic roles for innate are well-described multiple sclerosis (MS) its animal model, experimental autoimmune encephalomyelitis (EAE), protective aspects immune response less understood. In this study, we sought to identify function Dectin-1, a C-type lectin receptor (CLR), CNS autoimmunity. We found that Dectin-1 limited neuroinflammation EAE while canonical...