A5070616294- Immune Cell Function and Interaction
- Inflammasome and immune disorders
- Bone and Dental Protein Studies
- interferon and immune responses
- Immune cells in cancer
- Immune Response and Inflammation
- T-cell and B-cell Immunology
- Fungal Infections and Studies
- Antifungal resistance and susceptibility
- Autophagy in Disease and Therapy
- Neuroinflammation and Neurodegeneration Mechanisms
- Galectins and Cancer Biology
- Cancer Research and Treatments
- Immunotherapy and Immune Responses
- Tryptophan and brain disorders
- Cancer Immunotherapy and Biomarkers
- Immune responses and vaccinations
- Infant Nutrition and Health
- RNA Interference and Gene Delivery
- Plant Pathogens and Fungal Diseases
- Mycorrhizal Fungi and Plant Interactions
- Cytokine Signaling Pathways and Interactions
- Ubiquitin and proteasome pathways
- Mycobacterium research and diagnosis
- Phagocytosis and Immune Regulation
Duke University
2015-2024
Integra (United States)
2024
Laboratory of Molecular Genetics
2024
Duke University Hospital
2008-2023
Duke Medical Center
2008-2023
Institute of Neuroimmunology of the Slovak Academy of Sciences
2022
John Wiley & Sons (United States)
2021
Hudson Institute
2021
Durham University
2014
Dana-Farber Cancer Institute
2004-2010
Activation of CD4+ T cells results in rapid proliferation and differentiation into effector regulatory subsets. cell (Teff) (Th1 Th17) Treg subsets are metabolically distinct, yet the specific metabolic differences that modify populations uncertain. Here, we evaluated murine models determined inflammatory Teffs maintain high expression glycolytic genes rely on rates, while Tregs oxidative require mitochondrial electron transport to proliferate, differentiate, survive. Metabolic profiling...
The NLRP3 inflammasome is a multiprotein complex consisting of three kinds proteins, NLRP3, ASC, and pro-caspase-1, plays role in sensing pathogens danger signals the innate immune system. thought to be involved development experimental autoimmune encephalomyelitis (EAE), an animal model multiple sclerosis (MS). However, mechanism by which induces EAE not clear. In this study, we found that played critical inducing T-helper cell migration into CNS. To gain migratory ability, CD4 + T cells...
Stimulation of resting CD4 + T lymphocytes leads to rapid proliferation and differentiation into effector (Teff) or inducible regulatory (Treg) subsets with specific functions promote suppress immunity. Importantly, Teff Treg use distinct metabolic programs support subset specification, survival, function. Here, we describe that the orphan nuclear receptor estrogen-related receptor-α (ERRα) regulates pathways critical for Teff. Resting cells expressed low levels ERRα protein increased on...
Osteopontin (Opn) contributes to diverse biological processes that include immune responses, vascularization, and bone formation. Until recently, studies describing the activities of Opn have focused on cytokine-like properties secreted protein. Here, we show alternative translation a single mRNA species generates intracellular isoform. Utilization 5' canonical start site protein includes an N-terminal signal sequence allowing targeting secretory vesicles cytokine secretion, whereas usage...
Characterization of an animal model may explain why not all patients with multiple sclerosis respond to interferon-β.
Inflammasomes are cytosolic sensors that detect pathogens and danger signals in the innate immune system. The NLRP3 inflammasome is currently most fully characterized known to a wide array of microbes endogenous damage-associated molecules. Possible involvement (or inflammasomes) development multiple sclerosis (MS) was suggested number studies. Recent studies showed exacerbates experimental autoimmune encephalomyelitis (EAE), an animal model MS, although EAE can also develop without...
Upon activation, T cells require energy for growth, proliferation, and function. Effector (Teff) cells, such as Th1 Th17 utilize high levels of glycolytic metabolism to fuel proliferation In contrast, Treg oxidative suppressive It remains unknown how Teff/Treg-cell is altered when nutrients are limited leptin low. We therefore examined the role malnutrition associated hypoleptinemia on Teff versus cells. found that both malnutrition-associated cell-specific receptor knockout suppressed...
Immune responses must be well restrained in a steady state to avoid excessive inflammation. However, such restraints are quickly removed exert antimicrobial responses. Here we report role of autophagy an early host antifungal response by enhancing NFκB activity through A20 sequestration. Enhancement activation is achieved autophagic depletion A20, inhibitor, F4/80hi macrophages the spleen, peritoneum and kidney. We show that p62, adaptor protein, captures sequester it autophagosome. This...
The osteopontin (Opn) glycoprotein has been implicated in diverse physiological processes, including vascularization, bone formation, and inflammatory responses. Studies of its role immune responses suggested that Opn can set the early stage type-1 (cell-mediated) through differential regulation IL-12 IL-10 cytokine gene expression macrophages. Although to play a development immunity, little is known about control expression. Here, we report activated T cells, but not macrophages, regulated...
An endogenous circadian biological clock controls the temporal aspects of life in most organisms, including rhythmic control genes involved output pathways. In fungus Neurospora crassa , one pathway known to be under is asexual spore (conidia) development. To understand more fully processes that are regulated by N. clock, systematic screens were carried out for oscillate at transcriptional level. Time-of-day-specific cDNA libraries generated and used differential identify six new...
Summary Calcineurin plays essential roles in virulence and growth of pathogenic fungi is a target the natural products FK 506 C yclosporine A . In mucoralean fungus M ucor circinelloides , calcineurin mutation or inhibition confers yeast‐locked phenotype indicating that governs dimorphic transition. Genetic analysis this study reveals two catalytic subunits (out three) are functionally diverged. Homology modeling illustrates modes resistance resulting from amino substitutions interface...