A5087030841- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- Immune Cell Function and Interaction
- Fungal Infections and Studies
- RNA Interference and Gene Delivery
- Circular RNAs in diseases
- Immunotherapy and Immune Responses
- T-cell and B-cell Immunology
- Mycobacterium research and diagnosis
- Antifungal resistance and susceptibility
- Cytomegalovirus and herpesvirus research
- Reproductive System and Pregnancy
- Infant Health and Development
- Essential Oils and Antimicrobial Activity
- NF-κB Signaling Pathways
- Infant Nutrition and Health
- interferon and immune responses
- Cytokine Signaling Pathways and Interactions
- RNA Research and Splicing
- Immune cells in cancer
- Eosinophilic Esophagitis
- Antimicrobial Peptides and Activities
- Neonatal and fetal brain pathology
- IL-33, ST2, and ILC Pathways
Duke University
2021-2023
Beckman Research Institute
2017-2022
City of Hope
2015-2022
Duke Medical Center
2020-2021
Duke University Hospital
2021
City Of Hope National Medical Center
2019-2020
Institut de Biologie Moléculaire et Cellulaire
2017
Significance Aberrant inflammation is the root cause of numerous human diseases, from autoimmunity to cancer. microRNA-146a ( miR-146a ), a member large class small regulatory RNAs, functions as critical molecular brake on and malignant transformation. However, mechanism through which exerts its activity in immune cells unclear. Using mouse genetics, we have examined role miR-146a–Traf6 signaling axis found that although this miRNA–target interaction responsible for regulating normal myeloid...
Regulatory T (Treg) cells are critical in preventing aberrant immune responses. Posttranscriptional control of gene expression by microRNA (miRNA) has recently emerged as an essential genetic element for Treg cell function. Here, we report that mice with cell-specific ablation miR-142 (hereafter Foxp3CremiR-142fl/fl mice) developed a fatal systemic autoimmune disorder due to breakdown peripheral T-cell tolerance. displayed significant decrease the abundance and suppressive capacity cells....
Neutrophils are critical as the first-line defense against fungal pathogens. Yet, previous studies indicate that neutrophil function is complex during Cryptococcus neoformans (Cn) infection. To better understand role of neutrophils in acute pulmonary cryptococcosis, we analyzed heterogeneity by single-cell transcriptional analysis immune cells lung Cn -infected mice from a published dataset. We identified reference-based annotation and two distinct subsets generated infection: A subset with...
Intestinal immunity is coordinated by specialized mononuclear phagocyte populations, constituted a diversity of cell subsets. Although the subsets constituting network are thought to be similar in both small and large intestine, these organs have distinct anatomy, microbial composition, immunological demands. Whether distinctions demand organ-specific populations with dedicated roles unknown. Here we implement new strategy subset murine intestinal phagocytes identify two novel which...
Abstract MicroRNAs (miRNAs) are a class of powerful post-transcriptional regulators implicated in the control diverse biological processes, including regulation hematopoiesis and immune response. To define functions miR-142, which is specifically abundantly expressed cells, we created mouse line with targeted deletion this gene. Our analysis miR-142-/- mice revealed critical role for miRNA development homeostasis lymphocytes. Marginal zone B cells expand knockout spleen, while number T B1...
C-type lectin receptors (CLRs) play key roles in antifungal defense. CLR-induced NF-κB is central to CLR functions immunity, and thus, molecules that control the amplitude of could profoundly influence host defense against fungal pathogens. However, little known about mechanisms negatively regulate NF-κB, which act on family broadly directly acute CLR-signaling cascades remain unidentified.
Abstract Control of gene expression by microRNA (miRNA) has recently emerged as a critical mechanism that regulates B cell activation and function. However, the role specific miRNAs in this process is unclear. Using germline knockout (KO) mice, we have previously shown miR-142 ablation impairs humoral immune responses despite significant expansion compartment, suggesting for effector function terminal differentiation. To more precisely dissect miR 142 responses, developed an activated...
Abstract microRNA-146a (miR-146a) has been previously implicated as an essential molecular brake that prevents immune overreaction and malignant transformation by attenuating NF-κB signaling. miR-146a−/− mice display autoimmune disorder, also develop frank tumors in secondary lymphoid organs a progressive myelodysplastic syndrome (MDS) they age. miR-146a was proposed to exert its regulatory activity targeting Traf6 Irak1, genes which encode two key adapter proteins function upstream of...
Abstract MicroRNA-142 (miR-142) is a versatile posttranscriptional regulator that plays crucial role in the regulation of both innate and adaptive immune responses. Abrogation miR-142 expression mice results profound immunodeficiency characterized by hypoimmunoglobulinemia failure to mount robust humoral responses soluble antigen challenges. Paradoxically, miR-142−/− display significant expansion B cell compartment due accumulation immature mature lymphocytes. To better understand...
Abstract Regulatory T (Treg) cells are critical in preventing aberrant immune responses. Post-transcriptional gene control by microRNA (miRNA), a large class of small regulatory RNA, has recently emerged as key genetic element required for Treg cell function. Nevertheless, specific miRNA gene(s) that play vital role the regulation activity still unknown. Here we report mice with cell-specific ablation miR-142 (hereafter Foxp3CremiR-142fl/fl mice) developed fatal systemic autoimmune disorder...
Abstract microRNA-142 (miR-142) is expressed predominantly in cells of hematopoietic origin and plays a vital role the regulation innate adaptive immunity. We have previously shown that miR-142 ablation mice results marked expansion immature mature B cell compartments. To better understand ontogenesis, we examined early development null using Hardy fraction analysis. Our findings indicate deletion significantly upregulates frequency pro-B cells, large pre-B (Fractions B, C, E), while...
Abstract Control of gene expression by microRNA (miRNA) has recently emerged as a critical mechanism that regulates B cell activation and function. However, the role specific miRNAs in this process is unclear. Using germline knockout (KO) mice, we have previously shown miR-142 ablation impairs humoral immunity despite significant expansion compartment, suggesting for effector We found mice lacking cannot generate antibody responses upon antigen challenge display impaired germinal center (GC)...
Abstract Intestinal immunity is coordinated by specialized mononuclear phagocyte populations, constituted a diversity of cell subsets. Although the subsets constituting network are thought to be similar in both small and large intestine, these organs have distinct anatomy, microbial composition, immunological demands. Whether distinctions demand organ-specific populations with dedicated roles unknown. Here we implement new strategy subset murine intestinal phagocytes identify two novel which...