A5074130940- Immunotherapy and Immune Responses
- RNA Interference and Gene Delivery
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Calpain Protease Function and Regulation
- Retinoids in leukemia and cellular processes
- Cancer-related Molecular Pathways
- vaccines and immunoinformatics approaches
- Glioma Diagnosis and Treatment
- Glutathione Transferases and Polymorphisms
- Cancer Immunotherapy and Biomarkers
- Heat shock proteins research
- Genetic and phenotypic traits in livestock
- Genetic Mapping and Diversity in Plants and Animals
- Systemic Lupus Erythematosus Research
- Inflammatory Myopathies and Dermatomyositis
- Virus-based gene therapy research
- Tea Polyphenols and Effects
- HIV Research and Treatment
- Curcumin's Biomedical Applications
- Immune cells in cancer
- Biological Activity of Diterpenoids and Biflavonoids
- Hereditary Neurological Disorders
- Hops Chemistry and Applications
- Sirtuins and Resveratrol in Medicine
Regeneron (United States)
2023
Duke Medical Center
2013-2022
Duke University
2017-2021
Duke University Hospital
2014-2020
Rutgers, The State University of New Jersey
1992-2013
First Hospital of Jilin University
2013
Jilin University
2013
University of Portsmouth
2013
Washington University in St. Louis
2013
National Taiwan University Hospital
2006-2012
The effects of topical administration curcumin on the formation benzo[a]pyrene (B[a]P)–DNA adducts and tumorigenic activities B[a]P 7,12-dimethyl-benz[a]anthracene (DMBA) in epidermis were evaluated female CD-1 mice. Topical application 3 or 10 μmol 5 min prior to 20 nmol [3H]B[a]P inhibited [3H]B[a]P—DNA by 39 61% respectively. In a two-stage skin tumorigenesis model, backs mice once weekly for weeks followed week later promotion with 15 12-O-tetradecanoylpborbol-13-acetate (TPA) twice 21...
In a series of transgenic mice, the human tissue collagenase gene was expressed in suprabasal layer skin epidermis. Visually, mice had dry and scaly which upon histological analysis revealed acanthosis, hyperkeratosis, epidermal hyperplasia. At ultrastructural level, intercellular granular materials were absent epidermis but contact maintained through intact desmosomes. Despite diversity underlying etiologies, similar morphological hyperproliferative changes are observed diseases lamellar...
Efficacy of dendritic cell (DC) cancer vaccines is classically thought to depend on their antigen-presenting (APC) activity. Studies show, however, that DC vaccine priming cytotoxic T lymphocytes (CTLs) requires the activity endogenous DCs, suggesting exogenous DCs stimulate antitumor immunity by transferring antigens (Ags) DCs. Such Ag transfer functions are most commonly ascribed monocytes, implying undifferentiated monocytes would function equally well as a modality and need not be...
Previously, phenethyl isothiocyanate (PEITC) and dibenzoylmethane (DBM) had been shown to inhibit intestinal carcinogenesis in Apc(Min/+) mice. In this study, we investigated the chemopreventive efficacy of PEITC DBM azoxymethane (AOM)-initiated dextran sodium sulfate (DSS)-promoted colon cancer mouse model compare their potential vivo mechanisms leading chemoprevention. The mice were fed with diet supplemented 0.05% or 1% before after AOM initiation. Our results showed that AOM/DSS PEITC-...
Abstract Memory T cells are distinguished from naive by their rapid production of effector cytokines, although mechanisms for this recall response remain undefined. In study, we investigated transcriptional IFN-γ Ag-specific memory CD4 cells. cells, only occurred after sustained Ag activation and was associated with high expression the T-bet transcription factor required Th1 differentiation binding to promoter as assessed chromatin immunoprecipitation analysis. By contrast, immediate...
During ischemic stroke, occlusion of the cerebrovasculature causes neuronal cell death (infarction), but naturally occurring genetic factors modulating infarction have been difficult to identify in human populations. In a surgically induced mouse model we previously mapped Civq1 distal chromosome 7 as quantitative trait locus determining infarct volume. this study, genome-wide association mapping using 32 inbred strains and an additional linkage scan for volume confirmed that size is...
We previously developed integrase-defective lentiviral vectors (IDLVs) as an antigen delivery system for inducing strong and prolonged immunity in animal models. Here, we examined the association between persistence of expression durability immune response. Following a single intramuscular (i.m.) or subcutaneous (s.c.) injection IDLV delivering GFP mice, evaluated inflammation at site antigen-specific T cells early late time points. Durable was detected up to 90 days only after i.m....
The two segments of double-stranded RNA from infectious pancreatic necrosis virus Sp were cloned into the plasmid vector pUC8. Two sets overlapping clones identified by restriction enzyme and Southern blot analyses. Each these was shown Northern analysis to be exclusively related either segment A or B genomic RNA. entire lengths estimated 2.9 2.6 kilobases, respectively. Sequences viral cDNA subcloned bacteriophage T7 polymerase vectors pT71 pT72. activity single-stranded RNAs transcribed...
Abstract To identify genes involved in cerebral infarction, we have employed a forward genetic approach inbred mouse strains, using quantitative trait loci (QTL) mapping for infarct volume after middle artery occlusion. We had previously observed that is inversely correlated with collateral vessel density most strains. In this study, expanded the pool of allelic variation among classical strains by utilizing eight founder Collaborative Cross and found wild-derived strain, WSB/EiJ, breaks...
Each enantiomer of the diastereomeric pair bay-region dibenz[ a,h ]anthracene 3,4-diol-1,2-epoxides in which benzylic 4-hydroxyl group and epoxide oxygen are either cis (isomer 1) or trans 2) were evaluated for mutagenic activity. In strains TA 98 100 Salmonellatyphimurium , diol with (1 S ,2 R ,3 ,4 ) absolute configuration [(–)-diol epoxide-1] had highest Chinese hamster V-79 cells, [(+)-diol epoxide-2] The also appreciable activity, whereas other two enantiomers very low tumor studies,...
Undifferentiated monocytes can be loaded with tumor antigens (Ag) and administered intravenously to induce antitumor cytotoxic T lymphocyte (CTL) responses. This vaccination strategy exploits an endogenous Ag cross-presentation pathway, where Ag-loaded (monocyte vaccines) transfer their resident splenic dendritic cells (DC), which then stimulate robust CD8 + CTL In this study, we investigated whether monocyte in combination CDX-301, a DC-expanding cytokine Fms-like tyrosine kinase 3 ligand...
Intestinal immunity is coordinated by specialized mononuclear phagocyte populations, constituted a diversity of cell subsets. Although the subsets constituting network are thought to be similar in both small and large intestine, these organs have distinct anatomy, microbial composition, immunological demands. Whether distinctions demand organ-specific populations with dedicated roles unknown. Here we implement new strategy subset murine intestinal phagocytes identify two novel which...