A5059936430- DNA Repair Mechanisms
- Photosynthetic Processes and Mechanisms
- RNA Research and Splicing
- interferon and immune responses
- Mitochondrial Function and Pathology
- RNA regulation and disease
- Protist diversity and phylogeny
- Light effects on plants
- Chromosomal and Genetic Variations
- Genomics and Chromatin Dynamics
- Plant Genetic and Mutation Studies
- CRISPR and Genetic Engineering
- Food Quality and Safety Studies
- High Altitude and Hypoxia
- Microbial Metabolic Engineering and Bioproduction
- Protein Degradation and Inhibitors
- Telomeres, Telomerase, and Senescence
- Histone Deacetylase Inhibitors Research
- Biofuel production and bioconversion
- Ubiquitin and proteasome pathways
New York University
2020-2021
Université de Montréal
2014-2020
Failure to maintain organelle genome stability has been linked numerous phenotypes, including variegation and cytosolic male sterility (CMS) in plants, as well cancer neurodegenerative diseases mammals. Here we describe a next-generation sequencing approach that precisely maps characterizes DNA rearrangements single genome-wide experiment. In addition displaying global portraits of genomic instability, it surprisingly unveiled an abundance short-range Arabidopsis thaliana human organelles....
Telomerase can generate a novel telomere at DNA double-strand breaks (DSBs), an event called de novo addition. How this activity is suppressed remains unclear. Combining single-molecule imaging and deep sequencing, we show that the budding yeast telomerase RNA (TLC1 RNA) spatially segregated to nucleolus excluded from sites of repair in cell cycle-dependent manner. Although TLC1 accumulates nucleoplasm G1/S, Pif1 promotes localization G2/M. In presence DSBs, nucleolar most G2/M cells but...
Though it is an essential process, transcription can be a source of genomic instability. For instance, may generate RNA:DNA hybrids as the nascent transcript hybridizes with complementary DNA template. These hybrids, called R-loops, act major cause replication fork stalling and breaks. In this study, we show that lowering R-loop levels in plastids Arabidopsis thaliana reduces rearrangements mitigates plastid genome instability phenotypes. This effect observed on genome-wide scale, loss sigma...
Mitochondrial double-strand breaks (mtDSBs) are toxic lesions that compromise mitochondrial function. Mito-nuclear communication is essential to maintain cellular homeostasis, however, the nuclear response mtDSBs remains unknown. Using mitochondrial-targeted TALENs, we show activate a type I interferon evidenced by phosphorylation of STAT1 and activation stimulated genes (ISG). Following mtDNA break formation, BAK-BAX mediated herniation releases RNA cytoplasm trigger RIG-I/MAVS-dependent...
ABSTRACT The identification of structural variations in genomes using next-generation sequencing approaches greatly facilitates the study genetic and genomic diseases. data generated these also provide interesting new means to examine DNA repair, recombination, replication better understand sources instability. To utilize this data, we developed SCARR (Systematic Combination Alignments Recreate Rearrangements) identify rearrangements, used it occurrence orientation-reversing events human...