A5034842575- Glycosylation and Glycoproteins Research
- Monoclonal and Polyclonal Antibodies Research
- Galectins and Cancer Biology
- Cancer-related Molecular Pathways
- Signaling Pathways in Disease
- Protein purification and stability
- Enzyme Production and Characterization
- Microbial Metabolites in Food Biotechnology
- Macrophage Migration Inhibitory Factor
- Peptidase Inhibition and Analysis
- Gut microbiota and health
- Polysaccharides and Plant Cell Walls
- DNA Repair Mechanisms
- Microbial Metabolism and Applications
- Bacterial Identification and Susceptibility Testing
- Advanced Biosensing Techniques and Applications
- Food composition and properties
- Toxin Mechanisms and Immunotoxins
- RNA modifications and cancer
- Pharmacological Effects of Natural Compounds
Universidade Nova de Lisboa
2018-2023
Unidade em Ciências Biomoleculares Aplicadas
2021
Rede de Química e Tecnologia
2018-2021
University of Lisbon
2021
The p53 tumor suppressor is widely found to be mutated in human cancer. This protein regarded as a molecular hub regulating different cell responses, namely death. Compelling data have demonstrated that the impairment of activity correlates with development and maintenance. For these reasons, reactivation function promising strategy halt In present work, recombinant mutant p53R280K DNA binding domain (DBD) was produced for first time, its crystal structure determined absence resolution 2.0...
Abstract Overexpression of the Thomsen–Friedenreich (TF) antigen in cell membrane proteins occurs 90 % adenocarcinomas. Additionally, binding TF to human galectin‐3 (Gal‐3), also frequently overexpressed malignancy, promotes cancer progression and metastasis. In this context, structures that interfere with specific interaction have potential prevent A multidisciplinary approach combining optimized synthesis a mimetic NMR, X‐ray crystallography methods, isothermal titration calorimetry assays...
Abstract Glycan microarrays have played important roles in detection and specificity assignment of glycan recognition by proteins. However, the size diversity libraries current microarray systems are small compared to estimated glycomes, these may lead missed or incomplete assignment. For construction, covalent noncovalent immobilization two types methods used, but a direct comparison results from platforms is required. Here we develop chemical strategy prepare lipid-linked probes both...
Abstract Interactions of glycan‐specific epitopes to human lectin receptors represent novel immune checkpoints for investigating cancer and infection diseases. By employing a multidisciplinary approach that combines isothermal titration calorimetry, NMR spectroscopy, molecular dynamics simulations, X‐ray crystallography, we investigated the determinants govern recognition tumour pathogenic glycobiomarker LacdiNAc (GalNAcβ1‐4GlcNAc, LDN), including their comparison with ubiquitous LacNAc...
The Bacteroides thetaiotaomicron has developed a consortium of enzymes capable overcoming steric constraints and degrading, in sequential manner, the complex rhamnogalacturonan II (RG-II) polysaccharide. BT0996 protein acts initial stages RG-II depolymerisation, where its two catalytic modules remove terminal monosaccharides from side chains A B. is modular three putative carbohydrate-binding (CBMs) for which roles degradation are unknown. Here, we present characterisation module at...
A multigene polysaccharide utilization locus (PUL) encoding enzymes and surface carbohydrate (glycan)-binding proteins (SGBPs) was recently identified in prominent members of Bacteroidetes the human gut characterized Bacteroides ovatus. This PUL-encoded system specifically targets mixed-linkage β1,3-1,4-glucans, a group diet-derived carbohydrates that promote healthy microbiota have potential as prebiotics. The BoSGBPMLG-A protein encoded by BACOVA_2743 gene is SusD-like plays key role PUL's...