A5090228346- Ion channel regulation and function
- Neuroscience and Neuropharmacology Research
- Nicotinic Acetylcholine Receptors Study
- Pain Mechanisms and Treatments
- Force Microscopy Techniques and Applications
- Cellular Mechanics and Interactions
- Photoreceptor and optogenetics research
- Ion Transport and Channel Regulation
- Neuroscience and Neural Engineering
- Chemical Synthesis and Analysis
- Laser Applications in Dentistry and Medicine
- Biochemical effects in animals
- Pharmacological Receptor Mechanisms and Effects
- Lipid Membrane Structure and Behavior
- Neurobiology and Insect Physiology Research
- Neuropeptides and Animal Physiology
- Antimicrobial Peptides and Activities
- Mechanical and Optical Resonators
- Advanced Chemical Sensor Technologies
- Ocular and Laser Science Research
- Chemical Reactions and Isotopes
- Bioactive Compounds and Antitumor Agents
- Marine Toxins and Detection Methods
- Freshwater macroinvertebrate diversity and ecology
- Fusion and Plasma Physics Studies
Pavlov Institute of Physiology of the Russian Academy of Sciences
2015-2024
Russian Academy of Sciences
2010-2014
St Petersburg University
2000-2013
Institute of Experimental Medicine
2002
Effects of infrared (IR) radiation generated by a low-power Co2-laser on sensory neurons chick embryos were investigated organotypic culture method. Low-power IR firstly results in marked neurite suppressing action, probably induced activation Na+,K+-ATPase signal-transducing function. A further increase energy leads to stimulation growth. We suggest that this effect is triggered pumping Involvement the control transduction process was proved obtained after application ouabain at very low...
Effects of infrared (IR) radiation generated by a low-power Co2-laser on the membrane cultured dissociated nociceptive neurons newborn rat spinal ganglia were investigated using whole-cell patch-clamp method. Lowpower IR diminished voltage sensitivity activation gating machinery slow sodium channels (Nav1.8). Ouabain known to block both transducer and pumping functions Na+,K+-ATPase eliminated irradiation effects. The molecular mechanism interaction with sensory was proposed. primary event...
Comenic acid (CA), a specific agonist of opioid-like receptors, effectively and safely relieves neuropathic pain by decreasing the NaV1.8 channel voltage sensitivity in primary sensory neuron membrane. CA triggers downstream signaling cascades, which Na,K-ATPase/Src complex plays key role. After leaving complex, signal diverges 'tangentially' 'radially'. It is directed along membrane to channels, effective charge their activation gating system. In radial direction moving towards cell genome,...
In the primary sensory neuron, ouabain activates dual mechanism that modulates functional activity of Na V 1.8 channels. Ouabain at endogenous concentrations (EO) triggers two different signaling cascades, in which Na,K-ATPase/Src complex is EO target and signal transducer. The fast effect based on modulation channel activation gating device. tangential cascade along neuron membrane from Na,K-ATPase to channel. It evokes a decrease effective charge transfer Intracellular application PP2, an...
A gamma-pyrone derivative, comenic acid, activates the opioid-like receptor-mediated signaling pathway that modulates NaV1.8 channels in primary sensory neuron membrane. These are responsible for generation of nociceptive signal; therefore, gamma-pyrones have great therapeutic potential as analgesics, and this effect deserves a deeper understanding. The novelty our approach to design medicinal substance is based on combination data obtained from living neurons using very sensitive...
Low-power (non-thermal) infrared (IR) radiation with the wavelength of 10.6 μm activates Na,K-ATPase transducer function in sensory neurons, which is manifested decrease NaV1.8 channel voltage sensitivity at cellular membrane level and inhibition growth chick embryo dorsal root ganglia neurites tissue level. It shown that effect low-power IR totally blocked by a specific Src kinase inhibitor, PP2. Upon irradiation on background PP2, effective charge activation gating system does not differ...
Two short arginine-containing tripeptides, H-Arg-Arg-Arg-OH (TP1) and Ac-Arg-Arg-Arg-NH2 (TP2), have been shown by the patch-clamp method to modulate NaV1.8 channels of DRG primary sensory neurons, which are responsible for generation nociceptive signals. Conformational analysis tripeptides indicates that key role in ligand-receptor binding TP1 TP2 channel is played two positively charged guanidinium groups arginine side chains located at characteristic distance ~9 Å from each other. The...
Several arginine-containing short peptides have been shown by the patch-clamp method to effectively modulate NaV1.8 channel activation gating system, which makes them promising candidates for role of a novel analgesic medicinal substance. As demonstrated organotypic tissue culture method, all active and inactive studied do not trigger downstream signaling cascades controlling neurite outgrowth should be expected evoke adverse side effects on level upon their administration. The...
The signaling or non-pumping Na,K-ATPase function was first observed by us in the nociceptive neuron; transduced signals from opioid-like receptors to NaV1.8 channels. This study elucidates role of rhamnosyl residue ouabain activation function. effects resulting Ca2+ chelate complex (EO) are not manifested upon removal residue, as demonstrated viable cells highly sensitive patch-clamp and organotypic cell culture methods. Docking calculations show that is involved five intermolecular...
A possible molecular mechanism of the ligand-receptor binding Ac-Lys-Lys-Lys-NH
The present work continues our recent series of articles that aim to elucidate the ligand-receptor binding mechanism short cationic peptides NaV1.8 channel in nociceptive neuron. applied methodological approach has involved several methods: patch-clamp experimental evaluation effective charge activation gating system, organotypic tissue culture method, formalin test, and theoretical conformational analysis. lysine-containing peptide Ac-KEKK-NH2 been shown effectively modulate system. As...