A5042961228- Adenosine and Purinergic Signaling
- Neonatal Health and Biochemistry
- Calcium signaling and nucleotide metabolism
- Biochemical and Molecular Research
- PARP inhibition in cancer therapy
- Enzyme Structure and Function
- Diet, Metabolism, and Disease
- Cancer, Hypoxia, and Metabolism
- Amino Acid Enzymes and Metabolism
- Metabolism, Diabetes, and Cancer
- Bacterial Genetics and Biotechnology
- Pneumocystis jirovecii pneumonia detection and treatment
- Mitochondrial Function and Pathology
- Pancreatic function and diabetes
- Phenothiazines and Benzothiazines Synthesis and Activities
- RNA regulation and disease
- DNA and Nucleic Acid Chemistry
- Streptococcal Infections and Treatments
- Enzyme function and inhibition
- interferon and immune responses
- Genomics and Phylogenetic Studies
- Glycogen Storage Diseases and Myoclonus
- RNA modifications and cancer
- Drug Transport and Resistance Mechanisms
- Analytical Chemistry and Chromatography
Universidad de Extremadura
2014-2024
Washington State University
2009
Seoul National University
2009
University Hospital Complex Of Vigo
2006
Consejo Superior de Investigaciones Científicas
1985-1987
Universidade de Santiago de Compostela
1980
Flavins, comprising flavin mononucleotide (FMN), adenine dinucleotide (FAD), and riboflavin (RF, vitamin B2), play important roles in numerous redox reactions such as those taking place the electron-transfer chains of mitochondria all eukaryotes plastids plants. A selective chemosensor for flavins would be useful not only investigation metabolic processes but also diagnosis diseases related to flavins; a sensor is presently unavailable. Herein, we report first bifunctional (PTZ-DPA) flavins....
Ninety per cent of total rat liver hydrolytic activity (1.4 unitslg fresh tissue) on diadenosine or diguanosine 5',5"'-P',P4-tetraphosphate (Ap4A and Gp4G) present in isotonic homogenates sedimented at 37,000 x g.Supernatant corresponded to the earlier described, cytosolic specific, bis(5'-guanosyl) tetraphosphatase dinucleoside (EC 3.6.1.17;Lobaton, C. D., Vallejo, G., Sillero, A., M. A. G. (1975) Eur.J. Biochem.50,[495][496][497][498][499][500][501].Particulate activity, as extracted with...
The occurrence and distribution of bis-(5'-guanosyl) tetraphosphatase activity towards dinucleoside tetraphosphates between the 27 000 g supernatant sedimented fraction were studied in liver, kidney, brain, muscle intestinal mucosa from rat. p1p4-bis-(5'-guanosyl) tetraphosphate-hydrolysing activities found total homogenates 0.77, 1.44, 0.39, 0.36 2.14 units (mumol/min)/g respectively. 27000 g-sedimented fractions 74, 49, 11, 4 96% those present properties soluble enzymes investigated. All...
The cpdB gene is pro-virulent in avian pathogenic Escherichia coli and Salmonella enterica, where it encodes a periplasmic protein named CpdB. It structurally related to cell wall-anchored proteins, CdnP SntA, encoded by the also cdnP sntA genes of Streptococcus agalactiae suis, respectively. SntA effects are due extrabacterial hydrolysis cyclic-di-AMP, complement action interference. mechanism CpdB pro-virulence unknown, although from non-pathogenic E. hydrolyzes cyclic dinucleotides....
Mammalian triokinase, which phosphorylates exogenous dihydroxyacetone and fructose-derived glyceraldehyde, is neither molecularly identified nor firmly associated to an encoding gene. Human FMN cyclase, splits FAD other ribonucleoside diphosphate-X compounds monophosphate cyclic X-phosphodiester, identical a DAK-encoded kinase. This bifunctional protein was as triokinase. It modeled homodimer of two-domain (K L) subunits. Active centers lie between K1 L2 or K2 L1: binds K ATP L in different...
Streptococcus suis and agalactiae evade the innate immune system of infected host by mechanisms mediated cell wall-anchored proteins: SntA CdnP, respectively. The former has been reported to interfere with complement responses, latter dampens STING-dependent type-I interferon (IFN) response hydrolysis bacterial cyclic-di-AMP (c-di-AMP). Both proteins are homologous but, while CdnP studied as a phosphohydrolase, enzyme activities have not investigated. core structure was expressed in...
Endogenous cyclic diadenylate phosphodiesterase activity was accidentally detected in lysates of Escherichia coli BL21. Since this kind is uncommon Gram-negative bacteria, its identification undertaken. After partial purification and analysis by denaturing gel electrophoresis, renatured correlated with a protein identified fingerprinting as CpdB (cpdB gene product), which annotated 3´-nucleotidase / 2´,3´-cyclic-mononucleotide phosphodiesterase, it synthesized precursor signal sequence...
Two rat liver ADP‐ribose pyrophosphatases (ADPRibases) were partially purified. ADPRibase‐I hydrolyzed ( K m =0.5 μM) giving AMP as a product, required Mg 2+ or, less efficiently, Mn (Ca was not active), its activity changed little between pH 7 and 9, specific for it did hydrolyze ADP‐glucose, NAD + , NADH or diadenosine 5′,5″‐ P 1 n ‐ ‐phosphates (Ap 2 A, Ap 3 A). ADPRibase‐II showed similar properties, except that the 50 μM may be non‐specific, same preparation A. fulfils requirements of...
A study involving markers of subcellular and submitochondrial fractions, gradient centrifugation, latency measurements extraction with digitonin, demonstrates the association a specific ADP-ribose pyrophosphatase rat liver mitochondria its localization in matrix space. The enzyme hydrolyses to AMP, Km 2-3 microM. results support occurrence turnover pathway for free relevance mitochondria.
Nucleoside 5'-diphosphate-X hydrolases are interesting enzymes to study due their varied activities and structure-function relationships the roles they play in disposal, assimilation, modulation of effects substrates. Few these with a preference for CDP-alcohols known. In Yersinia intermedia suspensions prepared from cultures on Columbia agar 5% sheep blood, we found CDP-alcohol hydrolase liberated Triton X-100-containing medium. Growth at 25 degrees C was deemed optimum terms...
Background Triokinase and FMN cyclase (TKFC) is a bifunctional enzyme involved in fructose metabolism. catalyses the phosphorylation of fructose-derived glyceraldehyde (GA) exogenous dihydroxyacetone (DHA), while generates cyclic FMN. TKFC regulates antiviral immune response by interacting with IFIH1 (MDA5). Previously reported pathogenic variants are associated either multisystemic disease or isolated hypotrichosis loose anagen hairs. Methods Whole-exome sequencing identified homozygous...
Abstract Nucleotide-pyrophosphatases/phosphodiesterases (NPP/PDE) are membrane or secreted Zn 2+ -metallohydrolases of nucleoside-5´-monophosphate derivatives. They hydrolyze, for instance, ATP and 4-nitrophenyl-dTMP, belong to the ecto-nucleotide pyrophosphatase/phosphodiesterase (ENPP) family that contains seven members (ENPP1-ENPP7). Earlier we had shown an NPP/PDE activity solubilized partially purified from rat liver membranes is inactivated by EDTA in a time-dependent fashion, effect...
Abstract Rat liver dinucleoside triphosphatase (EC 3.6.1.29) is associated with sucrose-gradient purified mitochondria and can be extracted by freeze thaw treatment. The proportion of mitochondrial approaches 50% total enzyme. Evidence also presented that 10% bis(5'-guanosyl)tetraphosphatase 3.6.1.17) might equally linked to mitochondria. Those data suggest diadenosine 5',5'''-P1,P3-triphosphate, 5',5'''-P1,P4-tetraphosphate, or other substrates those enzymes, somehow related function(s),...
ADPRibase-Mn (Mn2+-dependent ADP-ribose/CDP-alcohol pyrophosphatase) was earlier isolated from rat liver supernatants after separation ADPRibase-I and ADPRibase-II (Mg2+-activated ADP-ribose pyrophosphatases devoid of CDP-alcohol pyrophosphatase activity). The last mentioned are putative Nudix hydrolases, whereas the molecular identity is unknown. MALDI (matrix-assisted laser-desorption ionization) MS data pointed to a hypothetical protein that cloned expressed showed expected specificity....
Loose anagen hair is a rare form of impaired anchorage in which hairs that lack inner and outer root sheaths can be gently painlessly plucked from the scalp. This condition usually occurs children often self-limiting. A genetic basis for disorder has been suggested but not proven. better understanding aetiology loose may improve prevention treatment strategies.To identify possible using next-generation DNA sequencing functional analysis variants identified.In this case study, whole-exome...
The earlier reported inhibition of rat liver nucleotide pyrophosphatase/phosphodiesterase I (EC 3.1.6.9/EC 3.1.4.1; NPP/PDE) by culture‐grade acidic fibroblast growth factor (FGF‐1) correlates with a low‐ M r contaminant. 1 H‐NMR analyses revealed EDTA in the total‐volume fractions gel‐filtration experiment, where all inhibitory activity FGF‐1 preparation was recovered. NPP/PDE (and unfractionated or EDTA‐containing fractions) time‐dependent, blocked substrate p ‐nitrophenyl‐dTMP, and...
Cyclic ADP‐ribose (cADPR) metabolism in mammals is catalyzed by NAD glycohydrolases (NADases) that, besides forming ADP‐ribose, form and hydrolyze the N 1 ‐glycosidic linkage of cADPR. Thus far, no cADPR phosphohydrolase was known. We tested rat ADP‐ribose/CDP‐alcohol pyrophosphatase (ADPRibase‐Mn) found that an ADPRibase‐Mn ligand substrate. activity on 65‐fold less efficient than best This similar to ADP‐ribose/cADPR formation ratio NADases. The product phosphohydrolysis...