Context: Exosomes secreted by tumor cells are a good source of cellular components that stimulate the immune response, such as alarmins (mRNA, tetraspanins (CD9, CD63, CD81), heat-shock proteins, major histocompatibility complex class I molecules) and tumor-associated antigens. These properties permit to pulsed dendritic in immunotherapy for many cancers types. The aim this study was demonstrate use exosomes derived from canine transmissible venereal (CTVT) an antigen its administration dogs...

The gut microbiota is involved in the productivity of beef cattle, but impact different analysis strategies on microbial composition unclear. Ruminal samples were obtained from Beefmaster calves (n = 10) at both extremes residual feed intake (RFI) values (5 with lowest and 5 highest RFI) two consecutive days. Samples processed using DNA extraction methods. V3 V4 regions 16S rRNA gene amplified PCR sequenced a MiSeq instrument (Illumina). We analyzed 1.6 million sequences all 40 (10 calves, 2...

Aflatoxins can cause intoxication and poisoning in animals humans. Among these molecules, aflatoxin B1 (AFB1) is the most dangerous because of its carcinogenic mutagenic properties. To mitigate effects, clay adsorbents are commonly included diet to adsorb carcinogens prevent their absorption gastrointestinal tract. In this study, four clays, three smectites (C-1, C-2, C-3), one zeolite (C-4), were compared as AFB1 trace inorganic nutrients using an vitro model for poultry. Characterization...

Canine transmissible venereal tumour (CTVT) is a sexually transmitted affecting dogs worldwide, imposing financial burden on dog owners. A stable culture cell line in continuous passages for >18 months has only been achieved once. The present study investigated CTVT isolated from bitch and its potential as vaccine.A biopsy 2-year-old mongrel with was obtained histopathological confirmation isolation of cells. cells were cultured to passage 55 characterised by flow cytometry, karyotyping...

A controlled, parallel, randomized and comparative trial was carried out to evaluate the anti-inflammatory efficacy of IMMUNEPOTENT CRP versus ibuprofen in patients after third-molar surgery over seven days. The evaluated using method Amin Laskin, analysis cytokine production (IL-2, IL-4, IL-6, IL-10, TNF-α, INF-γ) saliva done by flow cytometry. swelling process significant (p < 0.05) treatments with or controlled this properly; no difference between groups found 0.05). Both were shown...

As a class of secondary metabolites or toxins produced by fungi, aflatoxins can poison humans and animals; among them, aflatoxin B 1 (AFB ) is the most dangerous one owing to its carcinogenic mutagenic properties that increase risks for hepatocellular carcinoma in humans; hence, adsorbents such as smectites are commonly included poultry feed mitigate their effects. In this study, chitosan was crosslinked with sodium dodecyl sulfate (SDS) form an insoluble polymer complex stable at relevant...

The aim of the present study was to evaluate therapeutic potential autologous DCs loaded with whole tumor cell lysate CTVT generated under a simplified and rapid procedure in vitro production process, vulvar submucosal model dogs.We intravulvar dogs. A antigen prepared according method 1-butanol after administered complete Freund's adjuvant via subcutaneous female healthy dogs challenge cells corroborate immunogenicity. Short-time dendritic pulsed whole-lysate performed, analyzed by...

The use of goats for meat production faces challenges from environmental and nutritional factors. Urea is an affordable non-protein nitrogen source commonly utilized in ruminant nutrition. objective this study was to investigate utilization fed low-quality hay supplemented with molasses blocks containing urea. Twenty Anglo-Nubian doelings were individually housed metabolic cages provided chopped Buffelgrass (Cenchrus ciliaris) ad libitum. Goats randomly assigned four urea levels (0, 2, 4,...

The aim of this study was to determine the effects supplementing extruded and pelleted starter feeds on growth performance, rumen development, carcass characteristics, sensorial meat quality goat kids fed an all-milk protein replacer. Forty-five new-born French Alpine (21 males, 4.93 ± 0.58 kg; 24 females, 4.40 0.60 kg) were randomly assigned three treatment diets, milk replacer-only (RO), replacer supplemented with feed (RE), or (RP) in a 3 x 2 factorial arrangement. After 35 days, final...

Equine infectious anemia (EIA) is a highly disease in members of the Equidae family, caused by equine virus (EIAV). The severity ranges from subclinical to acute or chronic, and causes significant economic losses industry worldwide. Serologic tests for detection EIAV infection have some concerns given prolonged seroconversion time. Therefore, molecular methods are needed improve surveillance programs this disease. We attempted 6 clinical 42 non-clinical horses Nuevo Leon State, Mexico, using...

Background: Immunotherapy involving dendritic cells (DC) has been used to treat cancer with satisfactory results. The generation of mature derived from monocytes, however, is expensive because the use cytokines. Objective: To reduce DC therapy costs, it important evaluate lower-cost materials capable inducing cell maturation; for this purpose, we synthetized chitosan nanoparticles. Methods: Chitosan nanoparticles were by ionic gelation and characterized using dynamic light scattering, laser...

Most in vivo studies related to ruminal development calves use invasive techniques involving rumen-fistulated or euthanized animals. In consideration of animal welfare, we developed an oral endoscopic biopsy procedure allow the obtaining rumen epithelial samples, thus serving as alternative for measuring height and width papillae a safe, quick, efficient manner that allows slaughtering be avoided. This was tested on 12 Brangus crossbred randomly distributed two groups, with one fed meal...

The canine transmissible venereal tumor (CTVT) is the most common malignity in dogs. Because there are reports that this resistant to vincristine sulfate, chemotherapeutic options scarce, and development of new therapeutic approaches necessary. In study, we evaluated cytotoxic activity vincristine, doxorubicin, temozolomide, panobinostat, toceranib, gemcitabine, cisplatin, fluorouracil, cyclophosphamide, methotrexate on a CTVT cell line, determining all drugs decreased viability...

Blood samples were obtained from 16 high-risk heifers; eight newly arrived a 40 h road trip (0 days post-arrival (DPA)), whereas the other heifers had been in feedlot at 25 DPA. Both groups transported southeast tropical region of Mexico to northeast and sampled on same day. The complete blood count, chemistry, cytokine gene expression analyzed. Gene was analyzed using specific primers amplify quantify cDNA reverse transcribed mRNA transcripts for tumor necrosis factor (TNF)-α, interferon...

Bovine papillomatosis affects animal health and represents one of the greatest economic losses in livestock sector. New control prevention methods to protect industry from this disease are necessary. The aim study was evaluate a candidate peptide for antibody production against bovine papillomavirus (BPV).A total 64 cattle underwent wart excision among 5,485 distributed over 2 4 farms per state 12 four Mexican states Tabasco, Chiapas, Veracruz, Nuevo León. prevalence farm calculated by...