A5002804902- Glycosylation and Glycoproteins Research
- Injury Epidemiology and Prevention
- RNA modifications and cancer
- Trauma and Emergency Care Studies
- Gun Ownership and Violence Research
- Carbohydrate Chemistry and Synthesis
- Bone fractures and treatments
- Proteoglycans and glycosaminoglycans research
Case Western Reserve University
2022-2023
MetroHealth Medical Center
2022
Johns Hopkins University
2018-2020
In this report we use 'high-flux' tributanoyl-modified N-acetylmannosamine (ManNAc) analogs with natural N-acetyl as well non-natural azido- and alkyne N-acyl groups (specifically, 1,3,4-O-Bu3ManNAc, 1,3,4-O-Bu3ManNAz, 1,3,4-O-Bu3ManNAl respectively) to probe intracellular sialic acid metabolism in the near-normal MCF10A human breast cell line comparison earlier stage T-47D more advanced MDA-MB-231 cancer lines. An integrated view of was gained by measuring production tandem transcriptional...
Sialylation, a post-translational modification that impacts the structure, activity, and longevity of glycoproteins has been thought to be controlled primarily by expression sialyltransferases (STs). In this report we explore complementary impact metabolic flux on sialylation using glycoengineering approach. Specifically, treated three human breast cell lines (MCF10A, T-47D, MDA-MB-231) with 1,3,4-O-Bu3ManNAc, "high flux" precursor for sialic acid biosynthetic pathway. We then analyzed...