This paper describes a SlipChip to perform digital PCR in very simple and inexpensive format. The fluidic path for introducing the sample combined with mixture was formed using elongated wells two plates of designed overlap during loading. broken up by slipping that removed among brought each well contact reservoir preloaded oil generate 1280 reaction compartments (2.6 nL each) simultaneously. After thermal cycling, end-point fluorescence intensity used detect presence nucleic acid. Digital...

The actomyosin contractile ring is a network of cross-linked actin filaments that facilitates cytokinesis in dividing cells. Contractile formation has been well characterized Schizosaccharomyces pombe, which the cross-linking protein α-actinin SpAin1 bundles filament network. However, specific biochemical properties and whether they are tailored for not known. Therefore we purified quantified its ability to dynamically bind bundle vitro using combination bulk sedimentation assays direct...

We studied the self-assembly of inorganic nanocrystals (NCs) confined inside nanoliter droplets (plugs) into long-range ordered superlattices. showed that a capillary microfluidic platform can be used for optimization growth conditions NC superlattices and provide insights kinetics assembly process. The utility our approach was demonstrated by growing large (up to 200 μm) three-dimensional (3D) various NCs, including Au, PbS, CdSe, CoFe2O4. also it is possible grow 3D binary nanoparticle in plugs.

Despite the growing number of genome-wide association studies, involvement polymorphisms in microRNA target sites (polymiRTS) Alzheimer's disease (AD) remains poorly investigated. Recently, we have shown that AD-associated single-nucleotide (SNPs) present 3' untranslated region (3'UTR) amyloid precursor protein (APP) could directly affect miRNA function. In theory, loss (miRNA) function lead to risk for AD by increasing APP expression and Aβ peptide production. this study, tested hypothesis...

Introduction We developed an automated liquid chromatography-tandem mass spectrometry high performance chromatography tandem (HPLC-MS/MS) method for multiplex quantification of wild-type (wt) amyloid β (Aβ) peptides 1-40 (Aβ40) and 1-42 (Aβ42) detection variant Aβ in cerebrospinal fluid. Methods The HPLC-MS/MS assay was validated a clinically accredited laboratory following regulatory guidelines, with Aβ42 calibration assigned to the ERM/IFCC certified reference material; sequence variants...

The nematode C. elegans uses mechanosensitive neurons to detect bacteria, which are food for worms. These release dopamine suppress foraging and promote dwelling. Through a screen of genes highly expressed in dopaminergic food-sensing neurons, we identify K2P-family potassium channel-TWK-2-that damps their activity. Strikingly, loss TWK-2 restores mechanosensation lacking the NOMPC-like channel transient receptor potential 4 (TRP-4), was thought be primary mechanoreceptor tactile sensing....

Complement Receptor 1 (CR1) and CD2-associated protein (CD2AP) are two immune function genes identified in genome-wide association studies as risk factors for late onset Alzheimer Disease (LOAD). Additionally, a recent study suggested that missense single nucleotide polymorphism (SNP) CR1 (rs4844609, Thr1610Ser) may be causative pathological mutation associated with increase of AD. We investigated the LOAD SNPs (rs 6656401, rs3818361, rs4844609) CD2Ap (rs9349407) using large Canadian cohorts...

Vitamin D insufficiency has been associated with Alzheimer's disease (AD) and impaired cognition. Recent studies have also shown significant associations between vitamin receptor (VDR) single nucleotide polymorphisms (SNPs), AD cognitive decline. While Fok1 SNPs not directly implicated in AD, the TaubF haplotype (combined Taq1, Apa1, Tru91, Bsm1, Fok1) been. A recent study revealed a association global We hypothesized that executive functioning would differ among sought to examine...

Physical and optical properties of colloidal cadmium telluride nanocrystals (CdTe NCs) were investigated. The CdTe NCs synthesized by reacting elemental tellurium dissolved in tributylphosphine with a mixture oxide, octadecene, oleic acid. These NCs, which characterized transmission electron microscopy (TEM) are spherical ranged from 5 to 7 nm diameter. identity the compound post-synthesis was confirmed X-Ray diffraction (XRD) patterns. UV-Vis photoluminescence (PL) as grown pure samples...

Recent studies have suggested an association between low Vitamin D level and cognitive impairment. Some of the observed differences may be attributable to affinity vitamin receptor for D3 ligand. We hypothesize that genetic polymorphisms in influence our body response D, incur risk AD. In current study, we compared genotype frequencies a cohort AD patients with controls Canadian population. examined 8 SNPs receptor, including Fok1, Taq1, Apa1, Bsm1, Cdx, Tru91, rs7968585, rs7976091. There...

Until recently, quantitative analysis of amyloid-beta (Aβ) peptides in CSF had relied almost exclusively on the use immunometric assays. In order to side step known immunoassay reagent issues and heterophile antibody interference, we endeavored develop a fully automated liquid chromatography tandem mass spectrometry (LC-MS/MS) assay for Aβ40 Aβ42 peptides. Published LC-MS/MS methods date have focused wild-type Aβ peptides; however, familial mutations that occur within region could result...

Objective: To examine the correlation between four risk loci and clinical progression to Alzheimer9s Disease. Background: Recent genome-wide association studies have identified several new genetic for Disease (AD), including INPP5D (rs35349669), PTK2B (rs28834970), ZCWPW1 (rs1476679) TREM2 (rs75932628). Their utility in predicting outcome of patients with cognitive impairment has not been examined. We used two large Canadian cohorts study role these genes impairment. Methods: In current...

Recent genome-wide association studies identified several new genetic risk factors (CR1, CLU, PICALM, and TOMM40) for late-onset AD (LOAD). Their utility in predicting outcome of patients with cognitive impairment has not been examined depth. Here we used 2 large Canadian cohorts to study the role these genes impairment. The Study Health Aging (CSHA) is a population based longitudinal elderly follow-up from 1991 2001. There were 1728 subjects consented DNA testing. Collaborative Cohort...

Recent genome wide association studies (GWAS) revealed several new risk loci for late-onset Alzheimer disease (LOAD). We undertook genetic case-control to examine the role of 10 promising novel AD (CR1, CLU, PICALM, TOMM40, CD33, EPHA1, MS4A4A, CD2AP, BIN1, and ABCA7) in participants from 2 large cohorts: Canadian Study Health Aging (CSHA) A Collaborative Cohort Cognitive Impairment Related Dementia (ACCORD) using prevalent incident cases AD. included subjects (age ≥65) both cohorts. Those...

Recent studies have shown that carriers of GRN mutation a significantly lower level PGRN in plasma than non-carriers and controls. We measured the serum cohort subjects assessed at tertiary dementia referral clinic to determine its utility screening for carriers. Blood samples were obtained from with family history pathologically proven FTLD-U, as well patients cognitive complaints healthy carrier status “at-risk” members was determined by direct sequencing exonic flanking intronic regions....

Towards implementation in a routine clinical setting, we previously developed an automated high performance liquid chromatography—triple quadrupole mass spectrometry (HPLC-MS/MS) method for quantification of amyloid-β (Aβ) peptides Aβ40 and Aβ42 cerebrospinal fluid (CSF)(Van der Gugten, JG et al.,2016). This assay has been validated following Clinical Laboratory Standards Institute guidelines accredited hospital laboratory. With mind, evaluated pre-analytical analytical factors to optimize...

Current evidence suggests that Alzheimer disease (AD) and cerebrovascular (CVD) share a number of risk factors, including the well known vascular risks such as hypertension, diabetes, hypercholesterolemia, obesity. Recent genome wide association studies revealed several novel loci for diseases, in particular small vessels white matter disease, which often co-exists with AD. In current study, we undertook genetic case-control study from 3 Canadian cohorts to examine role ATP5H, ZCCHC14, PRKCH...