A5016756676- Hematopoietic Stem Cell Transplantation
- Electronic Packaging and Soldering Technologies
- Cellular Mechanics and Interactions
- Acute Lymphoblastic Leukemia research
- Immune Cell Function and Interaction
- Blood transfusion and management
- Fungal and yeast genetics research
- Neutropenia and Cancer Infections
- Analytical Chemistry and Sensors
- Microtubule and mitosis dynamics
- Intermetallics and Advanced Alloy Properties
- Metallurgical and Alloy Processes
- Childhood Cancer Survivors' Quality of Life
- Semiconductor materials and devices
- Advanced Fluorescence Microscopy Techniques
- T-cell and B-cell Immunology
- Acute Myeloid Leukemia Research
- Palliative Care and End-of-Life Issues
- Family Support in Illness
- Biocrusts and Microbial Ecology
- Pharmacy and Medical Practices
- Cancer Genomics and Diagnostics
- Gas Sensing Nanomaterials and Sensors
- Mesenchymal stem cell research
- CAR-T cell therapy research
National Marrow Donor Program
2018-2024
Center for Cancer and Blood Disorders
2021
Emory University
2021
University of Chicago
2013-2019
University of Minnesota
2018
Molecular Biology Consortium
2014
University of North Texas
1991-2002
Texas Instruments (United States)
1995-2000
Donor hematopoietic clones with pathogenic mutations engraft and persist in unrelated stem cell transplant recipients.
Actin regulators facilitate cell migration by controlling protrusion architecture and dynamics. As the behavior of individual actin becomes clear, we must address why cells require multiple with similar functions how they cooperate to create diverse protrusions. We characterized Diaphanous (Dia) Enabled (Ena) as a model, using complementary approaches: culture, biophysical analysis, Drosophila morphogenesis. found that Dia Ena have distinct biochemical properties contribute different...
The actomyosin contractile ring is a network of cross-linked actin filaments that facilitates cytokinesis in dividing cells. Contractile formation has been well characterized Schizosaccharomyces pombe, which the cross-linking protein α-actinin SpAin1 bundles filament network. However, specific biochemical properties and whether they are tailored for not known. Therefore we purified quantified its ability to dynamically bind bundle vitro using combination bulk sedimentation assays direct...
Abstract Natural killer (NK) cell recognition and killing of target cells are enhanced when inhibitory immunoglobulin-like receptors (KIR) unable to engage their cognate HLA class I ligands. The genes the KIR locus organized into either B haplotypes, containing 1 or more activating A which lack those genes. Analysis unrelated donor (URD) hematopoietic transplants (HCT), given acute myeloid leukemia (AML) patients between 1988 2009, showed that haplotype donors were associated with better...
Both de novo–assembled actin filaments at the division site and existing recruited by directional cortical transport contribute to contractile ring formation during cytokinesis. However, it is unknown which source more important. Here, we show that fission yeast formin For3 responsible for node condensation into clumps in absence of Cdc12. localization depended on F-BAR protein Cdc15, for3 deletion was synthetic lethal with mutations cause defects formation. became essential cells expressing...
The kinetics and morphologies of Cu deposition on HF‐treated silicon surfaces were investigated by atomic force microscopy (AFM), inductively coupled plasma mass spectroscopy (ICP/MS), graphite furnace absorption (GFAAS). early stage (<60 s) deposition, as characterized AFM, was found to be dominated the nucleation nanometer‐sized nuclei surfaces. After 60 s total grain number deposits leveled a constant plateau. However, significant size increase noticed. We employed an AFM...
The characterization of composite solders has been performed, specifically on tin/lead/Cu/sub 6/Sn/sub 5/ composition. This includes both microstructural and mechanical analysis. Scanning electron microscope (SEM) cross sections the solder was preferred over optical inspection, owing to similar appearance Sn Cu/sub phases by latter method. properties composites were examined found be superior those standard tin/lead solder. Melting points same as 60/40 Solder joints readily prepared using...
Bone marrow (BM) is an essential source of hematopoietic stem cell grafts for many allogeneic transplant (HCT) recipients, including adult patients (for specific diseases and transplantation strategies) the majority pediatric recipient. However, since advent granulocyte colony-stimulating factor-mobilized peripheral blood (PBSC) grafts, there has been a significant decrease in use BM HCT, thought to be due mainly increased logistical challenges harvesting compared with PBSCs, as well...
Abstract Multiple models of donor killer immunoglobulin receptor (KIR) alloreactivity or KIR genotype have been reported to be protective against leukemia relapse after allogeneic transplantation. However, few studies addressed this topic in the pediatric population. Here, we assessed outcomes transplantation children with acute lymphoblastic (ALL; n = 372) myeloid (AML; 344) who received unrelated (URD) and were Center for International Blood Marrow Transplant Research (CIBMTR) from 2005...
Abstract There are limited data on the effect of donor body mass index (BMI) peripheral blood stem cell (PBSC) mobilization response to granulocyte colony-stimulating factor (G-CSF), especially in unrelated donors. Obesity has been associated with persistent leukocytosis, elevated circulating progenitor cells, and enhanced mobilization. Therefore, we hypothesized that adequate collection CD34+ cells may be achieved lower doses (per kilogram weight) G-CSF donors higher BMI compared BMI. Using...
The evolutionarily conserved small actin-monomer binding protein profilin is believed to be a housekeeping factor that maintains general pool of unassembled actin. However, despite similar primary sequences, structural folds, and affinities for G-actin poly-L-proline, budding yeast ScPFY fails complement fission SpPRF temperature-sensitive mutant cdc3-124 cells. To identify profilin's essential properties, we built combinatorial library variants containing either WT or residues at multiple...
The regulated assembly of multiple filamentous actin (F-actin) networks from an monomer pool is important for a variety cellular processes. Chlamydomonas reinhardtii unicellular green alga expressing conventional and divergent that emerging system investigating the complex regulation polymerization. One network contains exclusively F-actin in fertilization tubule, mating structure at apical cell surface gametes. In addition to two genes, expresses profilin (PRF1) four formin genes (FOR1-4),...
This study aimed to identify risk factors for compartment syndrome (CS) in pediatric trauma populations.We included patients younger than 19 years treated at centers contributing the National Trauma Data Bank between 2009 and 2012. Multivariable logistic regression was used examine association development of CS. The final model adjusted age, sex, race, number comorbidities, Glascow Coma Scale, Injury Severity Score, mechanism injury, fracture lower limb.A total 341,238 were eligible...