A5090944141- Artificial Intelligence in Healthcare and Education
- Photochemistry and Electron Transfer Studies
- RNA Research and Splicing
- Spectroscopy and Quantum Chemical Studies
- RNA and protein synthesis mechanisms
- Metal complexes synthesis and properties
- DNA and Nucleic Acid Chemistry
- Lanthanide and Transition Metal Complexes
- Protein Interaction Studies and Fluorescence Analysis
- Surfactants and Colloidal Systems
- Magnetism in coordination complexes
- Bacterial Genetics and Biotechnology
- Health Systems, Economic Evaluations, Quality of Life
- Genomics and Chromatin Dynamics
- Meta-analysis and systematic reviews
- RNA modifications and cancer
- Retinoids in leukemia and cellular processes
- RNA Interference and Gene Delivery
- Hemoglobin structure and function
- Advanced biosensing and bioanalysis techniques
- Inflammasome and immune disorders
- Growth Hormone and Insulin-like Growth Factors
- Cancer, Hypoxia, and Metabolism
- Viral Infections and Vectors
- Chromosomal and Genetic Variations
Lancet Laboratories
2021-2024
Jain University
2024
Mid Yorkshire Hospitals NHS Trust
2023
Imperial College London
2013-2022
Digital Health Cooperative Research Centre
2021
University of Oklahoma
2016-2021
University of Nevada, Reno
2017
Jadavpur University
2013-2015
Cornell University
2012
National Institutes of Health
1986-2011
We have sequenced miRNA libraries from human embryonic, neural and foetal mesenchymal stem cells. report that the majority of genes encode mature isomers vary in size by one or more bases at 3′ and/or 5′ end miRNA. Northern blotting for individual miRNAs showed proportions isomiRs expressed a single gene often differ between cell tissue types. IsomiRs were readily co-immunoprecipitated with Argonaute proteins vivo active luciferase assays, indicating they are functional. Bioinformatics...
Drawing from real-life scenarios and insights shared at the RAISE (Responsible AI for Social Ethical Healthcare) conference, we highlight critical need in health care (AIH) to primarily benefit patients address current shortcomings systems such as medical errors access disparities. The embodying a sense of responsibility urgency, emphasized that AIH should enhance patient care, support professionals, be accessible safe all. discussions revolved around immediate actions leaders, adopting...
The ability to controllably handle the smallest materials is a fundamental enabling technology for nanoscience. Conventional optical tweezers have proven useful manipulating microscale objects but cannot exert enough force manipulate dielectric smaller than about 100 nm. Recently, several near-field trapping techniques been developed that can provide higher stiffness, they tend be limited in their reversibly trap and release due combination of extremely high electromagnetic fields resulting...
Magnetic force spectroscopy is a rapidly developing single molecule technique that found numerous applications at the interface of physics and biology. Since invention first magnetic tweezers, number modifications were incorporated into approach helped relieve limitations original design amplified its strengths. Inventive molecular biology solutions further advanced by expanding possible applications. In present form, method can be applied to molecules live cells without resorting intense...
Cell envelopes of many bacteria consist two membranes studded with efflux transporters. Such organization protects from the environment and gives rise to multidrug resistance. We report a kinetic model that accurately describes permeation properties this system. The predicts complex non-linear patterns drug uptake complete bifurcation, which recapitulate known experimental anomalies. introduce parameters, barrier constants, replace those Michaelis Menten for trans-envelope transport. Both...
We describe a new program called cryptic splice finder (CSF) that can reliably identify sites (css), so providing useful tool to help investigate splicing mutations in genetic disease. report many css are not entirely dormant and often already active at low levels normal genes prior their enhancement also fascinating correlation between the positions of introns, whereby within exons one species frequently match exact position introns equivalent from another species. These results strongly...
We have investigated the effect of surface charge on rate assembly ab dimers human hemoglobin A: a + B -5 a@.Heme intact BA subunits were compared with four mutant which differ by integral units charge: BN(Lys-95 Glu) ( 2 3 ; p(Gly-16 Asp) (1-); ,@(Glu-6 Val) (l+); BC(Glu-6 Lys) (2+).Subunit competition experiments performed as follows.Varying amounts 3H-labeled aA added to mixture containing equal
ZIKV causes microcephaly by crossing the placental barrier, however, mechanism of trans-placental dissemination remains unknown. Here, we sought to determine whether monocytes, which can cross tissue barriers, assist fetus. We determined this infecting monocytes with two strains ZIKV: South American (PRVABC59) and Nigerian (IBH30656) analyzing viral replication. found that infects replicates in macrophages, results modulation a large number cellular genes. Analysis these genes identified...
Abstract We have studied intercalation of ethidium bromide (EB) to genomic DNA encapsulated in a nanospace an anionic AOT reverse micelle (RM). Circular dichroism (CD) study on the RM reveals its condensed form. Here, we used temporal decay‐associated spectra (DAS) and time‐resolved area normalized emission spectral (TRANES) techniques investigate EB‐binding because interference from unbound EB makes conventional steady state picosecond resolved fluorescence spectroscopic challenging. The...
We report structural and dynamical aspects of DNAs from various sources including synthetic oligonucleotides in bulk buffer as a complex with histone1 (H1). High-resolution transmission electron microscopic (HRTEM) studies reveal the change upon complexation H1 leading to formation compact-globular hollow-toroidal particles. In order explore functionality ligand binding their complexes H1, we have used two biologically common fluorescent probes Hoechst 33258 (H33258) Ethidium (EB) model...
Dinuclear rhenium(I) complexes having a fac-[Re(CO)3](+) moiety of general formula fac-[Re2(CO)6(hq)2] have been synthesized in excellent yield by reacting [Re(CO)5Cl] with Hhq ratio 1 : toluene an argon atmosphere. Here hq(-) is the deprotonated form 5-phenylazo-8-hydroxyquinoline (Hhq(1)), 5-(2-naphthylazo)-8-hydroxyquinoline (Hhq(2)) and 5-(2-fluorineazo)-8-hydroxyquinoline (Hhq(3)). The reaction dinuclear imidazole (Im) N-methylimidazole (N-MeIm) dry dichloromethane under atmosphere...
Two mononuclear Re(v) complexes were synthesized using two different <italic>O</italic>, <italic>N</italic>, <italic>N</italic> coordinating Schiff base ligands. The X-ray structure and catalytic properties of the are investigated electronic absorption spectra also calculated.
The stoichiometric reaction of [Re(CO)5Cl] with 2-(arylazo)phenyl benzyl thioethers [ArNNC6H4SCH2Ph: Ar = Ph, 4-chlorophenyl, 4-nitrophenyl] (L1–L3) in a ratio 1 : dry toluene under refluxing condition afforded the Re(I) complexes general formula fac-[Re(L)(CO)3Cl] excellent yields. ligands used this present work are 2-(phenylazo)phenyl thioether (L1), 2-(4-chlorophenylazo)phenyl (L2) and 2-(4-nitrophenylazo)phenyl (L3). elemental analysis ESI mass spectroscopic measurements ensure formation...
Transgenic technologies conventionally rely on the oocyte as a substrate for genetic modification. Owing to their accessibility, however, male germ cells, including mature sperm, have material advantages use in transgenesis. Here we exploited lentiviruses generate transgenic animals via germline. When pseudotyped lentiviral vectors encoding green fluorescent protein (GFP) were incubated with mouse spermatozoa, these sperm highly successful producing transgenics. Lentivirally transduced...
In this report, the validity and divergence of activation energy barrier crossing model for bound to free type water transition at interface AOT/lecithin mixed reverse micelle (RM) has been investigated first time in a wide range temperatures by time-resolved solvation fluorophores. Here, picosecond-resolved dynamics two fluorescent probes, ANS (1-anilino-8-naphthalenesulfonic acid, ammonium salt) Coumarin 500 (C-500), RM have carefully examined 293, 313, 328, 343 K. Using dynamic light...
Fluorescent carbon nanoparticle (CNP) having 2-6 nm in size with quantum yield of about ~3% were synthesized via nitric acid oxidation soot and this approach can be used for milligram scale synthesis these water soluble particles. These CNPs are nano-crystalline predominantly graphitic structure shows green fluorescence under UV exposure. While induces nitrogen oxygen incorporation into particle that afforded solubility light emitting property; the isolation small particles from a mixture...