A5071626682- Sulfur Compounds in Biology
- Nitric Oxide and Endothelin Effects
- Endoplasmic Reticulum Stress and Disease
- Redox biology and oxidative stress
- Electron Spin Resonance Studies
- Cholinesterase and Neurodegenerative Diseases
- Advanced Glycation End Products research
- Phosphodiesterase function and regulation
- Sphingolipid Metabolism and Signaling
- Viral Infectious Diseases and Gene Expression in Insects
- Advanced Chemical Sensor Technologies
- Blood properties and coagulation
- Adenosine and Purinergic Signaling
- Constructed Wetlands for Wastewater Treatment
- Enzyme function and inhibition
- Phosphorus and nutrient management
- Hemoglobin structure and function
- Advanced Nanomaterials in Catalysis
- Eicosanoids and Hypertension Pharmacology
- Folate and B Vitamins Research
- Enzyme Catalysis and Immobilization
- Antiplatelet Therapy and Cardiovascular Diseases
- Biochemical effects in animals
- Autophagy in Disease and Therapy
- Odor and Emission Control Technologies
University of Windsor
2015-2025
University of Science and Technology Beijing
2010
World Precision Instruments (United States)
2010
Western University
2009
Windsor Regional Hospital
2007
Concordia University
2002
Wayne State University
2000-2002
UNSW Sydney
2001
Istituti di Ricovero e Cura a Carattere Scientifico
2000
Massachusetts General Hospital
1999
N-dansylhomocysteine (DnsHCys) is quenched on S-nitrosation. The product of this reaction, N-dansyl-S-nitrosohomocysteine, a sensitive, direct fluorogenic substrate for the denitrosation activity protein disulfide isomerase (PDI) with an apparent K(M) 2 microM. S-nitroso-BSA (BSA-NO) competitively inhibited reaction K(I) 1 oxidized form DnsHCys, N,N-didansylhomocystine, rapidly accumulated in cells and was reduced to DnsHCys. fluorescence DnsHCys-preloaded human umbilical endothelial hamster...
Abstract Some aspects of the physiological role NO may be mediated by stable NO‐carriers such as S ‐nitrosoglutathione and related ‐nitrosothiols. In this report we show that irradiation S‐nitrosoglutathione at either absorption band (λ max = 340 nm or 545 nm) results in release nitric oxide. Photolysis exhibited a quantum yield 0.056 ± 0.002 was best approximated first‐order process with k obs 4.9 × 10 −7 0.3 s −1 . The photolytic from resulted an enhanced cytotoxic effect on HL‐60 leukemia...
Various Mannich bases of chalcones and related compounds displayed significant cytotoxicity toward murine P388 L1210 leukemia cells as well a number human tumor cell lines. The most promising lead molecule was 21 that had the highest activity cells. In addition, exerted preferential toxicity to lines compared transformed T-lymphocytes. Other interest were 38, with huge differential in between cells, 42, high therapeutic index when Molt 4/C8 T-lymphocytes compared. general, more cytotoxic...
S-nitrosoglutathione (GSNO) denitrosation activity of recombinant human protein disulfide isomerase (PDI) has been kinetically characterized by monitoring the loss S-NO absorbance, using a NO electrode, and with aid fluorogenic NOx probe 2,3-diaminonaphthalene. The initial rates as function [GSNO] displayed hyperbolic behavior irrespective method used to monitor denitrosation. Km values estimated for GSNO were 65 +/- 5 microm 40 10 in bond production (NO electrode or 2,3-diaminonaphthalene),...
Hydrogen sulfide (H2S) has recently been recognized as an important physiologically relevant gasotransmitter. Produced by the enzymes involved in transsulfuration pathway, cystathionine β-synthase (CBS) and γ-lyase (CSE), H2S implicated to control biological activity virtually every organ system. In recent years it is being that many commonly used assays do not measure free specifically may be prone artifacts. This led large variations reported concentrations. order accurately study H2S’s...
Disulfide-linked gold nanoparticles (AuNP) were synthesized by reacting dithiobis[succinimidylpropionate] (DSP) coated with glutathione disulfide. AuNP-cross-linking was monitored the red shift and broadening of AuNP's localized surface plasmon absorption resonance (LSPR) spectrum. The exposure disulfide-linked AuNPs to a variety free thiols systematically varying molecular weight revealed AuNP-disulfide stability reduction up critical weight, Mc, >300 Da thus making AuNP first reagent that...
Hydrogen (H2(g)) is a viable green fossil fuel alternative, as its combustion yields only water and energy. However, H2(g) highly flammable, explosive, lacks odor. These characteristics warrant sensitive specific detection methods for widespread use an alternative source. Recently, there has been growing interest in the development of sensors, particularly those that are easy to use, environmentally friendly, sensitive. Here, we show first example optical fluorogenic hydrogen sensing...
Abstract— Laser flash photolysis of S‐nitroso complexes glutathione (GSNO) and bovine serum albumin (BSANO) via excitation at 355 nm has been used to investigate the photogeneration nitric oxide (NO) subsequent radical reactions. In case GSNO, liberation NO was confirmed by its oxidation oxyhemoglobin met hemoglobin. Initial release is homolytic cleavage S‐N bond produce thiyl radical, GS, which can subsequently react with (a) ground‐state GSNO ( k = 1.7 × 10 9 M −1 /i> s ) yield...
We present a simple method by which gold nanoparticles (AuNPs) are used to simultaneously isolate and enrich for free or modified thiol-containing peptides, thus facilitating the identification of protein S-modification sites. Here, disulfide isomerase (PDI) dual specificity phosphatase 12 (DUSP12 hYVH1) were S-nitrosylated S-glutathionylated, their thiols differentially alkylated, subjected proteolysis. AuNPs added digests, AuNP-bound peptides isolated centrifugation released thiol...
Summary Background. The purpose of this study was to probe the pleiotrophic effects Atorvastatin on intraplatelet-nitric oxide metabolism. Methods and Results. Hyperlipidemic subjects (n = 19) were treated for 1 month (following a 3-week washout) with either or placebo in double-blinded randomized 2, crossover), placebo-controlled study. Changes levels intraplatelet nitric synthase, nitrotyrosine correlated cholesterol, LDL-C, HDL-C triglyceride levels. These studies indicate that...
S-nitrosothiols (RSNOs) regulate several aspects of platelet physiology including inhibition activation, adhesion and aggregation. PDI (protein disulphide-isomerase) has recently been found to be localized the cell surface, where it exhibits both disulphide-exchange denitrosation activities. The activity linked present study suggests that metabolism RSNOs by platelets is a function activity. Exposure washed human increasing concentrations GSNO (S-nitrosoglutathione) resulted in saturable...
The kinetic consequences of acetylcholinesterase peripheral site occupation by metal ions were examined using three substrates; acetylthiocholine, p-nitrophenylacetate, and 7-(dimethylcarbamoyloxy)-N-methylquinolinium iodide. Two classes ion effects noted: activation a group including Mg 2+ , Ca Mn Na + inactivation second which to date includes Zn Cd Hg Ni Cu Pb . Activation is demonstrable only in solutions low ionic strength whereas can be readily observed both high strength. appears due...
Nitric oxide (NO) signaling is inextricably linked to both its physical and chemical properties. Due preferentially hydrophobic solubility, NO molecules tend partition from the aqueous milieu into biological membranes. We hypothesized that plasma membrane ordering provided by cholesterol further couples physics of diffusion with cellular signaling. Fluorescence lifetime quenching studies pyrene liposome preparations showed presence decreased apparent coefficients approximately 20-40%,...
Nitrite (NO(2)(-)) recycling to nitric oxide (NO) is catalysed by a number of enzymes and induces protective vasodilation effect under hypoxia/ischaemia. In the present work, we tested in vitro ability three NOS (nitric synthase) isoforms release NO from nitrite anoxia using electrochemical detection, chemiluminescence absorption spectroscopy. The free anoxic solutions at 15 muM was specific endothelial isoform (eNOS) did not occur with neuronal (nNOS) or inducible (iNOS) isoforms. Unlike...
Hydrogen sulfide (H(2)S) is known to play a physiological role in processes as diverse vasodilation, maintenance of vascular tone, neurotransmission, and immune response. The multitude functions which H(2)S involved warrants the development useful methods for its detection. Here, we introduce simple continuous detection method that exploits relatively high polydimethylsiloxane (PDMS) permeability comparison other thiols typically encountered cellular milieu. In this method, 96-well inserts...