A5087360626- RNA Research and Splicing
- Genomics and Chromatin Dynamics
- RNA and protein synthesis mechanisms
- Fungal and yeast genetics research
- RNA modifications and cancer
- Plant Gene Expression Analysis
- Toxoplasma gondii Research Studies
- Genomics and Phylogenetic Studies
- Epigenetics and DNA Methylation
University of Geneva
2014-2024
Recent studies have suggested that a sub-complex of RNA polymerase II composed Rpb4 and Rpb7 couples the nuclear cytoplasmic stages gene expression by associating with newly made mRNAs in nucleus, contributing to their translation degradation cytoplasm. Here we show yeast two hybrid co-immunoprecipitation experiments, followed ribosome fractionation fluorescent microscopy, subunit Ccr4-Not complex, Not5, is essential nucleus for functions Rpb4. Not5 interacts Rpb4; it required presence...
The current understanding of gene expression considers transcription and translation to be independent processes. Challenging this notion, we found that efficiency is determined during elongation through the imprinting mRNAs with Not1, central scaffold Ccr4-Not complex. We another subunit complex, Not5, defines Not1 binding specific mRNAs, particularly those produced from ribosomal protein genes. This mechanism specifically regulates expression, which in turn determines translational...
Acetylation of histones regulates gene expression in eukaryotes. In the yeast Saccharomyces cerevisiae it depends mainly upon ADA and SAGA histone acetyltransferase complexes for which Gcn5 is catalytic subunit. Previous screens have determined that global acetylation reduced cells lacking subunits Ccr4–Not complex, a regulator eukaryotic expression. this study we characterized functional connection between complex SAGA. We show mRNAs encoding core set are tethered together co-translational...
Abstract The phylum Apicomplexa comprises eukaryotic parasites that cause fatal diseases affecting millions of people and animals worldwide. Their mitochondrial genomes have been significantly reduced, leaving only three protein-coding genes highly fragmented mitoribosomal rRNAs, raising challenging questions about mitoribosome composition, assembly structure. Our study reveals how Toxoplasma gondii assembles over 40 mt-rRNA fragments using exclusively nuclear-encoded proteins...
Histone turnover during gene transcription is mediated by regulatory proteins, not the machinery.
The NOT genes encode subunits of the conserved Ccr4-Not complex, a global regulator gene expression, and in particular mRNA metabolism. They were originally identified selection for increased resistance to histidine starvation yeast S. cerevisiae. Recent work indicated that Not5 subunit, ortholog mammalian CNOT3, determines translation levels by defining binding scaffold protein Not1 ribosomal mRNAs during transcription. This is needed optimal proteins. In this we searched mutations budding...