A5067923963- Coccidia and coccidiosis research
- Parasitic Infections and Diagnostics
- Helminth infection and control
- Animal Nutrition and Physiology
- Toxoplasma gondii Research Studies
- Veterinary medicine and infectious diseases
- Cytomegalovirus and herpesvirus research
- Herpesvirus Infections and Treatments
- Rabbits: Nutrition, Reproduction, Health
- Trypanosoma species research and implications
- Insect and Pesticide Research
- Rabies epidemiology and control
- Viral gastroenteritis research and epidemiology
- Research on Leishmaniasis Studies
- Bacteriophages and microbial interactions
- Pharmacological Effects and Assays
- Bird parasitology and diseases
- Biochemical and Molecular Research
- Parasite Biology and Host Interactions
- Parasites and Host Interactions
- Insect symbiosis and bacterial influences
- Virus-based gene therapy research
- Metal and Thin Film Mechanics
- Immune Cell Function and Interaction
- Vector-borne infectious diseases
China Agricultural University
2016-2025
Ministry of Agriculture and Rural Affairs
2015-2024
Shijiazhuang Academy of Agriculture and Forestry Sciences
2024
Hebei Academy of Agriculture and Forestry Sciences
2024
Faculty of Public Health
2024
China National Center for Food Safety Risk Assessment
2024
Ministry of Public Security of the People's Republic of China
2023
Iowa State University
2016
National Animal Husbandry Service
2013
Hebei North University
2012
Phytoplasmas, the causal agents of numerous plant diseases, are insect-vector-transmitted, cell-wall-less bacteria descended from ancestral low-G+C-content Gram-positive in Bacillus–Clostridium group. Despite their monophyletic origin, widely divergent phytoplasma lineages have evolved adaptation to specific ecological niches. Classification and taxonomic assignment phytoplasmas been based primarily on molecular analysis 16S rRNA gene sequences because inaccessibility measurable phenotypic...
Grape seed proanthocyanidin extract (GSPE) has been widely used as a human food supplement for health promotion and disease prevention. However, there was little information regarding its application in animal nutrition. The aim of the current study is to determine effect GSPE at different concentrations on chicken performance, status antioxidant/oxidant system after Eimeria tenella infection. In first experiment, incorporated diet 5, 10, 20, 40, 80 mg/kg significantly decreased mortality...
Phytoplasmas are cell wall-less bacteria that cause numerous diseases in several hundred plant species. During adaptation to transkingdom parasitism diverse and insect hosts, phytoplasma evolution has given rise widely divergent lineages. Since phytoplasmas cannot be cultured a cell-free medium, measurable phenotypic characters suitable for conventional microbial classification mostly inaccessible. Currently, differentiation mainly dependent on restriction fragment length polymorphism (RFLP)...
Abstract Coccidiosis, an intestinal disease caused by Eimeria protozoan parasites, affects various animal species, and especially poses a significant threat to the poultry industry. The current primary control methods include anticoccidial drugs vaccines. However, emerging challenges such as drug resistance vaccine efficacy issues are rooted in complex life cycle species diversification of . In this review, we first consolidate recent breakthroughs understanding biology, focusing on parasite...
The phylum Apicomplexa includes parasites of medical, zoonotic and veterinary significance. Understanding the global distribution genetic diversity these protozoa is fundamental importance for efficient, robust long-lasting methods control. Eimeria spp. cause intestinal coccidiosis in all major livestock animals are most important domestic chickens terms both economic impact animal welfare. Despite having significant negative impacts on efficiency food production, many questions relating to...
Yield and quality are fundamental features for any researchers during nucleic acid extraction. Here, we describe a simplified, semi-unified, effective, toxic material free protocol extracting DNA RNA from different prokaryotic eukaryotic sources exploiting the physical chemical properties of acids. Furthermore, this showed that under triple protection (i.e. EDTA, SDS NaCl) lysis step, environment is improper RNase to have liberated even DNase degrade DNA. Therefore, complete removal...
Abstract Better understanding of the molecular changes associated with disease is essential for identifying new routes to improved therapeutics and diagnostic tests. The aim this study was investigate dynamic in metabolic profile mouse sera during T. gondii infection. We carried out untargeted metabolomic analysis collected from female BALB/c mice experimentally infected Pru strain (Genotype II). Serum samples were at 7, 14 21 day post infection (DPI) control subjected liquid...
Diarrhea remains one of the leading causes morbidity and mortality globally, with enterotoxigenic Escherichia coli (ETEC) constituting a major causative pathogen. The development alternative treatments for diarrhea that do not involve chemotherapeutic drugs or result in antibiotic resistance is critical. Considering lysozyme naturally occurring antimicrobial peptide, previous study we developed transgenic pig line expresses recombinant human (hLZ) its milk. In present study, examined...
Proteins of all living cells undergo a myriad post-translational modifications (PTMs) that are critical to multifarious life processes. In this study, we describe the first comprehensive multiple PTM-omics atlas in parallel with quantitative proteome analyses two representative species African trypanosomes, Trypanosoma brucei and evansi. Ten PTM types approximately 40,000 modified sites 150 histone marks fine map on each protein trypanosomes were accomplished. The biologically different...
The apicomplexan parasites Eimeria spp. are the causative agents of coccidiosis, a disease with significant global impact on poultry industry. complex life cycle involves exogenous (sporogony) and endogenous (schizogony gametogony) stages. Unfortunately, genetic regulation these highly dynamic processes, particularly for genes involved in specific developmental phases, is not well understood.
Abstract Background Infection with the protozoan Toxoplasma gondii causes serious public health problems and is of great economic importance worldwide. Protection from acute toxoplasmosis known to be mediated by CD8+ T cells, but T. antigens host genes required for eliciting protective immunity have been poorly defined. The dense granule protein 6 (GRA6), recently proved highly immunogenic produces fully immune protection in infected BALB/c mice an H-2L d gene. cell response strain seemed...
Abstract Eimeria tenella, one of the seven species chicken coccidia, elicits protective immunity against challenge infection with both homologous and heterologous strains. We endeavor to use recombinant E. tenella as a vaccine vehicle for expressing delivering pathogen Ags investigate immune responses these foreign Ags. In this study, two lines transgenic model Ag, enhanced yellow fluorescent protein (EYFP), targeted micronemes cytoplasm parasites were constructed study impact Ag...
ABSTRACT Coccidiosis, caused by different species of Eimeria parasites, is an economically important disease poultry and livestock worldwide. Here we report previously unknown alterations in the gut microbes metabolism BALB/c mice infected with falciformis . Specifically, observed a significant shift abundance cecal bacteria disrupted parasitized animals. The relative abundances Lachnospiraceae bacterium NK4A136, Ruminiclostridium , Alistipes Lactobacillus declined response to E. infection,...
Malaria parasites and related haemosporidian are widespread may cause severe diseases in birds. These pathogens should be considered projects aiming breeding of birds for purposes sustained ex situ conservation. Cranes the 'flagship species' health assessment wetland ecosystems, majority species endangered. other haemosporidians have been reported cranes, but host-parasite relationships remain insufficiently understood. Morbidity cranes due to malaria has Beijing Zoo. This study report...
Coccidiosis is one of the most serious diseases livestock and birds in world. Vaccination with live-parasite anticoccidial vaccines genetic manipulation improving immunogenicity vaccine strains would be best means for controlling coccidiosis breeder layer stocks, even fast-growing broilers. Profilin from apicomplexan parasites first molecularly defined ligand Toll-like receptor 11 (TLR11) TLR12 mice a potential molecular adjuvant. Here, we constructed transgenic Eimeria tenella line...
The "self-cleaving" 2A sequence of picornavirus, which mediates ribosome-skipping events, enables the generation two or more separate peptide products from one mRNA containing sequences. In this study, we introduced a single porcine teschovirus-1 (P2A) linked to fluorescent protein genes, enhanced yellow (EYFP) gene and red (RFP) gene, in cassette into transgenic Eimeria tenella (EtER). As expected, obtained separated molecules rather than fused protein, although were translated same...