A5022708781- DNA and Nucleic Acid Chemistry
- DNA Repair Mechanisms
- Vector-borne infectious diseases
- Parasitic Infections and Diagnostics
- Coccidia and coccidiosis research
- Viral Infections and Vectors
- Vector-Borne Animal Diseases
- Chemical Synthesis and Analysis
- CRISPR and Genetic Engineering
- RNA and protein synthesis mechanisms
- Bacterial Genetics and Biotechnology
- Protein Tyrosine Phosphatases
- Protein Kinase Regulation and GTPase Signaling
- Toxoplasma gondii Research Studies
- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- RNA Research and Splicing
- Carbohydrate Chemistry and Synthesis
- Advanced biosensing and bioanalysis techniques
- Herpesvirus Infections and Treatments
- interferon and immune responses
- Ferroptosis and cancer prognosis
- SARS-CoV-2 and COVID-19 Research
- Biochemical and Molecular Research
- HIV/AIDS drug development and treatment
Rice University
2019-2025
Zhongshan Hospital
2022-2024
Fudan University
2022-2024
China Agricultural University
2022-2024
The First Affiliated Hospital, Sun Yat-sen University
2022-2024
Sun Yat-sen University
2022-2024
Jinshan Hospital of Fudan University
2018-2023
Yangzhou University
2021-2023
First Affiliated Hospital of Anhui Medical University
2023
Anhui Medical University
2023
A hit-and-run metal ion DNA polymerase is an enzyme that uses existing as a template to build new by adding nucleotides the end of newly forming daughter strand. The mechanism catalyzes formation phosphodiester bond known require two Mg 2+ ions, and recent crystal structures have shown third present after formation. Gao et al. used time-resolved crystallography visualize enzyme-substrate complex captures cation before occurs, synthesis cannot occur without ion. Binding this requires thermal...
Structures of the simplest replisome The DNA performs concerted parental-strand separation and synthesis on both strands. Gao et al. report cryo–electron microscopy structures minimum set bacteriophage T7 proteins that can carry out leading- lagging-strand at replication fork (see Perspective by Li O'Donnell). Three key enzymes involved in replication—DNA polymerase, helicase, primase—were visualized complex with substrate DNA, demonstrating their highly dynamic organizations Comparison...
Phlorizin, as a flavonoid from wide range of sources, is gradually becoming known for its biological activity. Phlorizin can exert antioxidant effects by regulating the IL-1β/IKB-α/NF-KB signaling pathway. At same time, it exerts antibacterial activity reducing intracellular DNA agglutination, protein and energy synthesis, destroying metabolism. In addition, phlorizin also has various pharmacological such antiviral, antidiabetic, antitumor, hepatoprotective effects. Based on domestic foreign...
Abstract Coccidiosis, an intestinal disease caused by Eimeria protozoan parasites, affects various animal species, and especially poses a significant threat to the poultry industry. The current primary control methods include anticoccidial drugs vaccines. However, emerging challenges such as drug resistance vaccine efficacy issues are rooted in complex life cycle species diversification of . In this review, we first consolidate recent breakthroughs understanding biology, focusing on parasite...
DNA polymerase θ (Pol θ) is an A-family specialized in double-strand breaks repair and translesion synthesis. Distinct from its high-fidelity homologs replication, Pol catalyzes template-dependent synthesis with inherent propensity for error incorporation. However, the structural basis of θ's low-fidelity not clear. Here, we present cryo-electron microscopy structures detailing domain human complex a cognate C:G base pair (bp), mismatched T:G bp, or T:T bp. Our illustrate that snugly...
Abstract Error-free replication of DNA is essential for life. Despite the proofreading capability several polymerases, intrinsic polymerase fidelity in general much higher than what base-pairing energies can provide. Although researchers have investigated this long-standing question with kinetics, structural determination, and computational simulations, factors that dictate are not fully resolved. Time-resolved crystallography has elucidated correct nucleotide incorporation established a...
Abstract The CRISPR-Cas13 ribonucleases have been widely applied for RNA knockdown and transcriptional modulation owing to their high programmability specificity. However, the large size of Cas13 effectors non-specific cleavage upon target activation limit adeno-associated virus based delivery systems therapeutic applications. Herein, we report detailed biochemical structural characterizations a compact (Cas13bt3) suitable delivery. Distinct from many other systems, Cas13bt3 cleaves...
Hepatocyte growth factor (HGF) stimulates mitogenesis, motogenesis, and morphogenesis in a wide range of cellular targets during development, homeostasis tissue regeneration. Inappropriate HGF signaling occurs several human cancers, the ability to initiate program protease production, cell dissociation, motility has been shown promote invasion is strongly linked tumor metastasis. Upon binding, tyrosines within intracellular domain its receptor, c-Met, become phosphorylated mediate binding...
Prenylated indole alkaloids featuring spirooxindole rings possess a 3R or 3S carbon stereocenter, which determines the bioactivities of these compounds. Despite stereoselective advantages biosynthesis compared with those organic synthesis, biocatalytic mechanism for controlling 3S-spirooxindole formation has been elusive. Here, we report an oxygenase/semipinacolase CtdE that specifies construction in 21R-citrinadin A. High-resolution X-ray crystal structures substrate and cofactor, together...
Significance To determine if there is a general base in the DNA synthesis reaction that deprotonates nucleophile, we systematically removed potential hydrogen-bond acceptors of 3′-OH primer strand. We then characterized activity human Pol η by kinetic, structural, and molecular dynamics simulation analyses. found no single or combined perturbations eliminate catalysis. Moreover, removal two proton mutating conserved S113 to Ala addition 2′-F end rescued defects S113A mutation alone. Our...
Name your splice: FR901464 analogues and herboxidiene inhibit constitutive splicing, most likely by inhibiting spliceosomal subunit SF3b. A parallel comparison of these compounds in a cell-based assay system showed meayamycin B as the potent splicing inhibitor among small molecules.
ABT-737 inhibits the anti-apoptotic proteins B-cell lymphoma 2 (BCL-2) and BCL-XL. Meayamycin B switches splicing pattern of myeloid cell leukemia factor 1 (MCL1) pre-mRNA. Specifically, inhibition 3B subunit (SF3B1) with meayamycin promotes generation proapoptotic, short variant (MCL1-S) diminishes antiapoptotic, long (MCL1-L). This action was previously associated cytotoxicity in non-small lung carcinoma lines. induced apoptosis response to an ablation MCL1-L by B. In this study, we...
In this study, blood samples obtained from 162 dogs in Jiangxi, China, were employed molecular screening of canine tick-borne pathogens by PCR and sequencing. Babesia spp. gene fragment was detected 12 (7.41%) dogs. All negative for Hepatozoon spp., Ehrlichia canis, Coxiella Borrelia Rickettsia Anaplasma platys. Species-specific analysis further confirmed that 8 (4.94%) 4 (2.47%) infected canis vogeli gibsoni, respectively. Based on our analyses, infection Jiangxi appeared not related to...