A5027556680- Advanced biosensing and bioanalysis techniques
- Electrochemical sensors and biosensors
- Biosensors and Analytical Detection
- CRISPR and Genetic Engineering
- Advanced Nanomaterials in Catalysis
- Advancements in Transdermal Drug Delivery
- SARS-CoV-2 detection and testing
- Electrochemical Analysis and Applications
- Analytical Chemistry and Sensors
- Microfluidic and Capillary Electrophoresis Applications
- Neuroscience and Neural Engineering
- Conducting polymers and applications
- RNA and protein synthesis mechanisms
- Molecular Junctions and Nanostructures
- MXene and MAX Phase Materials
- Dermatology and Skin Diseases
- Analytical chemistry methods development
- Layered Double Hydroxides Synthesis and Applications
- Traditional Chinese Medicine Analysis
- Advanced Biosensing Techniques and Applications
- Karst Systems and Hydrogeology
- Polymer Surface Interaction Studies
- RNA Interference and Gene Delivery
- Lipid Membrane Structure and Behavior
- Advanced Sensor and Energy Harvesting Materials
University of Connecticut
2022-2025
First People's Hospital of Chongqing
2025
State Key Laboratory of Food Science and Technology
2023-2024
Nanchang University
2023-2024
Beijing University of Chinese Medicine
2023
China Medical University
2023
Chinese Academy of Inspection and Quarantine
2023
Jilin University
2019-2023
Chongqing Medical University
2022
Daping Hospital
2022
The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) systems have recently received notable attention for their applications in nucleic acid detection. Despite many attempts, the majority of current CRISPR-based biosensors infectious respiratory disease diagnostic still require target preamplifications. This study reports a new biosensor amplification-free detection via harnessing trans-cleavage mechanism Cas13a and ultrasensitive graphene...
Quantitative polymerase chain reaction as a powerful tool for DNA detection has been pivotal to vast range of applications, including disease screening, food safety assessment, environmental monitoring, and many others. However, the essential target amplification step in combination with fluorescence readout poses significant challenge rapid streamlined analysis. The discovery engineering clustered regularly interspaced short palindromic repeats (CRISPR) CRISPR-associated (Cas) technology...
Neurochemical corelease has received much attention in understanding brain activity and cognition. Despite many attempts, the multiplexed monitoring of coreleased neurochemicals with spatiotemporal precision minimal crosstalk using existing methods remains challenging. Here, we report a soft neural probe for neurochemical via electrografting-assisted site-selective functionalization aptamers on graphene field-effect transistors (G-FETs). The probes possess excellent flexibility, ultralight...
Silicone elastomers, such as poly(dimethylsiloxane) (PDMS), have a broad range of applications in basic biomedical research and clinical medicine, ranging from the preparation microfluidic devices for organs-on-chips ventriculoperitoneal shunts treatment hydrocephalus to implantable neural probes neuropharmacology. Despite importance, protein adsorptions on silicone elastomers these application environments represent significant challenge. Surface coatings with slippery lubricants, inspired...
There is a strong demand for bioanalytical techniques to rapidly detect protease activities with high sensitivity and specificity. This study reports an activity-based electrochemical method toward this goal. Nanoelectrode arrays (NEAs) fabricated embedded vertically aligned carbon nanofibers (VACNFs) are functionalized specific peptide substrates containing ferrocene (Fc) tag. The kinetic proteolysis curves measured continuously repeated ac voltammetry, from which the catalytic activity...
In transdermal drug delivery systems, the physicochemical properties of affect its percutaneous permeability. However, whether drugs change their permeability in presence pores solid microneedles (MNs) has been less studied this area. project, cinnamaldehyde, curcumin, ferulic acid and geniposide were selected as model for study under action MNs, a combination classical experiments visualization means such scanning electron microscopy laser confocal was used to investigate...
To study the hydrochemical characteristics, controlling factors, and groundwater quality of Tan-Lu fault zone (Anhui section), 86 samples were taken from areas surrounding which included Jianghuai Wavy Plain, Yanjiang Hill Dabie Mountains in western Anhui. Descriptive statistics, Piper diagram, Gibbs ion ratio analysis, saturation index, chloride-alkalinity entropy weight water index (EWQI) used to comprehensively characteristics factors evaluate its quality. The results showed that shallow...
A controllable crRNA self-transcription aided dual-amplified CRISPR-Cas12a strategy (termed CST-Cas12a) was developed for highly sensitive and specific biosensing of flap endonuclease 1 (FEN1), a structure-selective nuclease in eukaryotic cells. In this strategy, branched DNA probe with 5′ overhanging designed to serve as hydrolysis substrate FEN1. The cut by FEN1 annealed template functioned primer an extension reaction produce double-stranded (dsDNA) containing T7 promoter transcription...
Wearable and implantable bioelectronics that can interface for extended periods with highly mobile organs tissues across a broad pH range would be useful various applications in basic biomedical research clinical medicine. The encapsulation of these systems, however, presents major challenge, as such devices require superior barrier performance against water ion penetration challenging environments while also maintaining flexibility stretchability to match the physical properties surrounding...