A5086593171- Vector-borne infectious diseases
- Vector-Borne Animal Diseases
- Viral Infections and Vectors
- Mosquito-borne diseases and control
- Trypanosoma species research and implications
- Salmonella and Campylobacter epidemiology
- Insect symbiosis and bacterial influences
- Viral gastroenteritis research and epidemiology
- Insect and Pesticide Research
- Antibiotic Resistance in Bacteria
- Helminth infection and control
- Biosensors and Analytical Detection
- Parasite Biology and Host Interactions
- Research on Leishmaniasis Studies
- Parasites and Host Interactions
- Milk Quality and Mastitis in Dairy Cows
- Parasitic Infections and Diagnostics
- Probiotics and Fermented Foods
- Microbial infections and disease research
- Plant Virus Research Studies
- Insect Pest Control Strategies
- Livestock and Poultry Management
- Escherichia coli research studies
- Pharmaceutical and Antibiotic Environmental Impacts
- Nematode management and characterization studies
North-West University
2016-2025
Central University of Technology
2024
Advanced Neural Dynamics (United States)
2021
National Biodiversity Institute
2020
South African National Biodiversity Institute
2020
University of the Free State
2004-2017
Obihiro University of Agriculture and Veterinary Medicine
2004-2012
Makerere University
2012
Kyambogo University
2012
National Agricultural Research Institute
2012
Globally, the spread of multidrug-resistant Pseudomonas aeruginosa, Escherichia coli, and Klebsiella pneumoniae from food to humans poses a severe threat public health. The aim this study was assess co-occurrence colistin β-lactamase resistance genes in E. K. pneumoniae, P. aeruginosa strains isolated faeces abattoir broiler chickens. isolates were successfully detected faecal samples by polymerase chain reaction (PCR) at infection rates 60.7%, 22.5% 16.7% respectively. displayed highest...
ABSTRACT A loop-mediated isothermal amplification (LAMP) procedure for the detection of Cryptosporidium in environmental and fecal samples was developed evaluated. This is first demonstration LAMP applied to . Due its specificity simplicity, method could become a useful diagnostic tool epidemiologic studies presence.
Three methods, microscopy, nested polymerase chain reaction (nPCR), and loop-mediated isothermal amplification (LAMP) have been applied for malaria diagnosis in 105 human blood samples collected Northern Thailand. Only Plasmodium falciparum vivax infections were detected. A total number of 57 positives (54%) could be detected P. 25 (24%) when all that positive any the three methods are counted together. The nPCR was used as a reference standard comparison with other microscopy LAMP....
Ticks carry and transmit a remarkable array of pathogens including bacteria, protozoa viruses, which may be veterinary and/or medical significance. With little to no information regarding the presence tick-borne zoonotic or their known vectors in southern Africa, aim our study was screen for Anaplasma phagocytophilum, Borrelia burgdorferi, Coxiella burnetii, Rickettsia species Ehrlichia ruminantium ticks collected identified from ruminants Eastern Cape, Free State, KwaZulu-Natal Mpumalanga...
Staphylococcus species are amongst the bacteria that cause bovine mastitis worldwide, whereby they produce a wide range of protein toxins, virulence factors, and antimicrobial-resistant properties which enhancing pathogenicity these organisms. This study aimed to detect spp. from milk cattle with subclinical using MALDI-TOF MS 16S rRNA PCR as well screening for antimicrobial resistance (AMR) genes. Our results uncovered 166 sampled cows, only 33.13% had after initial screening, while...
Abstract Background The Shiga toxin (Stx)-producing Escherichia coli (STEC) have become important global public health concerns. This study investigated the prevalence, antimicrobial resistance profile, and extended-spectrum beta-lactamase-producing E. in sheep goat faeces. Methods results A total of 53 isolates were confirmed by PCR targeting uidA [ β -D glucuronidase] gene. genes stx 1 2, as well bfp A, vir , eae lt aaf II virulence genes, detected this study. Of to be STEC, 100% positive...
This study evaluated the stability of LAMP reagents when stored at 25 degrees C and 37 C, also assessed its detection efficiency on different DNA template preparations. Accordingly, using amplified in vitro cultured T. b. brucei (GUTat 3.1) from day 1 to 15 reagent storage. There were no significant differences (P>0.05) sensitivity among -20 (recommended storage temperature). above-mentioned temperatures serially diluted DNAs (genomic extracted by phenol-chloroform method, FTA card hemolysed...
Lymphatic filariasis is a condition where the skin thickens and hardens after excessive swelling associated with lymphoedema. The caused by infection parasites classified as nematodes. In this study, an ethnobotanical survey of medicinal plants used against lymphatic in Eastern Cape, Free State, KwaZulu-Natal Mpumalanga Provinces South Africa was conducted through use structured questionnaires. Information gathered from 21 traditional healers, 9 herbalists 12 elderly people. respondents were...