A5035633093- Alzheimer's disease research and treatments
- Parkinson's Disease Mechanisms and Treatments
- Cellular transport and secretion
- Plant tissue culture and regeneration
- Lipid Membrane Structure and Behavior
- Neuroinflammation and Neurodegeneration Mechanisms
- Microtubule and mitosis dynamics
- Advanced Fluorescence Microscopy Techniques
- Botulinum Toxin and Related Neurological Disorders
- GABA and Rice Research
- Autophagy in Disease and Therapy
- Neurological disorders and treatments
- Trace Elements in Health
- Cholinesterase and Neurodegenerative Diseases
- Ethnobotanical and Medicinal Plants Studies
- Plant-Microbe Interactions and Immunity
- Cell Adhesion Molecules Research
- Prion Diseases and Protein Misfolding
- Plant Pathogenic Bacteria Studies
- Ginkgo biloba and Cashew Applications
- Enzyme Production and Characterization
- Endoplasmic Reticulum Stress and Disease
- Neuroscience and Neuropharmacology Research
- RNA Interference and Gene Delivery
- Caveolin-1 and cellular processes
Manipal Academy of Higher Education
2019-2025
KU Leuven
2009-2025
Institute for Stem Cell Biology and Regenerative Medicine
2019-2024
National Institute of Mental Health and Neurosciences
2024
Indian Institute of Science Bangalore
2024
Jawaharlal Nehru Centre for Advanced Scientific Research
2024
Hallym University
2024
Silchar Medical College and Hospital
2023
Joint Genome Institute
2014-2020
Gauhati Medical College and Hospital
2016-2020
Alzheimer's disease (AD) is the major cause of dementia. During development AD, neurofibrillary tangles progress in a fixed pattern, starting transentorhinal cortex followed by hippocampus and cortical areas. In contrast, deposition β-amyloid (Aβ) plaques, which are other histological hallmark does not follow same strict spatiotemporal it correlates poorly with cognitive decline. Instead, soluble Aβ oligomers have received increasing attention as probable inducers pathogenesis. this study,...
The spreading of pathology through neuronal pathways is likely to be the cause progressive cognitive loss observed in Alzheimer's disease (AD) and other neurodegenerative diseases. We have recently shown propagation AD via cell-to-cell transfer oligomeric amyloid beta (Aβ) residues 1-42 (oAβ1-42) using our donor-acceptor 3-D co-culture model. now show that different Aβ-isoforms (fluorescently labeled 1-42, 3(pE)-40, 1-40 11-42 oligomers) can from one cell another. Thus, not restricted a...
Our previous studies demonstrated that fluorescent early endocytic vesicles prepared from rat liver after injection of Texas red asialoorosomucoid contain asialoglycoprotein and its receptor move undergo fission along microtubules using kinesin I KIFC2, with Rab4 regulating KIFC2 activity (J. Cell Sci. 116, 2749, 2003). In the current study, procedures to prepare late were devised. addition, flow cytometry was utilized highly purified vesicles, permitting validation microscopy-based...
Plants respond to bacterial infection acutely with actin remodeling during plant immune responses. The mechanisms by which virulence factors (VFs) modulate polymerization remain enigmatic. Here, we show that plant-type-I formin serves as the molecular sensor for in response two VFs: Xanthomonas campestris pv. (Xcc) diffusible signal factor (DSF), and pathogen-associated pattern (PAMP) flagellin pattern-triggered immunity (PTI). Both VFs regulate assembly tuning clustering nucleation activity...
The hallmarks of Alzheimer disease are amyloid-β plaques and neurofibrillary tangles accompanied by signs neuroinflammation. Lysozyme is a major player in the innate immune system has recently been shown to prevent aggregation amyloid-β1-40 vitro. In this study we found that patients with have increased lysozyme levels cerebrospinal fluid co-localized plaques. Drosophila neuronal co-expression amyloid-β1-42 reduced formation soluble insoluble species, prolonged survival improved activity...
Harnessing the biotechnological potential of large number proteins available in sequence databases requires scalable methods for functional characterization. Here we propose a workflow to address this challenge by combining phylogenomic guided DNA synthesis with high-throughput mass spectrometry and apply it systematic characterization GH1 β-glucosidases, family enzymes necessary biomass hydrolysis, an important step conversion lignocellulosic feedstocks fuels chemicals. We synthesized...
Transmembrane adhesion receptors at the cell surface, such as CD44, are often equipped with modules to interact extracellular matrix (ECM) and intracellular cytoskeletal machinery. CD44 has been recently shown compartmentalize membrane into domains by acting pickets, facilitating function of signaling receptors. While spatial organization diffusion studies proteins usually conducted separately, here we combine observations using high spatio-temporal resolution imaging on living cells reveal...
Early endocytic vesicles loaded with Texas Red asialoorosomucoid were prepared from mouse liver. These bound to microtubules in vitro, and upon ATP addition, they moved bidirectionally, frequently undergoing fission into two daughter vesicles. There was no effect of vanadate (inhibitor dynein) on motility, whereas 5'-adenylylimido-diphosphate (kinesin inhibitor) highly inhibitory. Studies specific antibodies confirmed that dynein not associated these Kif5B the minus-end kinesin Kifc1...
Quorum sensing (QS) is a recognized phenomenon that crucial for regulating population-related behaviors in bacteria. However, the direct specific effect of QS molecules on host biology largely understudied. In this work, we show molecule DSF ( cis -11-methyl-dodecenoic acid) produced by Xanthomonas campestris pv. can suppress pathogen-associated molecular pattern–triggered immunity (PTI) Arabidopsis thaliana , mediated flagellin-induced activation flagellin receptor FLS2. The DSF-mediated...
The interaction of amyloid-β (Aβ) peptides with the plasma membrane (PM) is a potential trigger that initiates formation higher-order aggregates, alterations/damage, and progressive neurotoxicity in Alzheimers disease (AD). In previous study, we showed oligomers Aβ1-42 (oAβ1-42) induced PM damage, resulting repair cascade via lysosomal exocytosis coupled endocytosis, facilitation tunneling nanotubes (TNTs)-like protrusions to promote direct cell-to-cell transfer aggregates. this demonstrated...
Intracellular trafficking regulates the abundance and therefore activity of transporters present at plasma membrane. The transporter, Na+-taurocholate co-transporting polypeptide (ntcp), is increased membrane upon treatment cells with cAMP, for which microtubules (MTs) are required PI3K pathway PKCzeta have been implicated. However, ntcp on MTs has not demonstrated directly regulation intracellular localization well understood. Here, we utilize in vitro whole-cell immunofluorescence...
Tunnelling nanotubes (TNTs) are an emerging route of long-range intercellular communication that mediate cell-to-cell exchange cargo and organelles contribute to maintaining cellular homeostasis by balancing diverse stresses. Besides their role in communication, TNTs implicated several ways health disease. Transfer pathogenic molecules or structures via can promote the progression neurodegenerative diseases, cancer malignancy, spread viral infection. Additionally, acquiring resistance...
Methods for induction of callus and cell suspension cultures have been developed the medicinally important herb Centella asiatica (L.) Urban. Thin layer chromatography (TLC) high performance liquid (HPLC) analysis showed presence asiaticoside in vitro grown leaves, cultured cells.
One of the hallmarks neurodegenerative dementia diseases is progressive loss mental functions and ability to manage activities daily life. This progression caused by spread disease more brain areas via anatomical connections. The pathophysiological process responsible for this has long been sought after. There an increased understanding that driving force these could be small, soluble intraneuronal accumulations proteins rather than large, extracellular accumulations. Recently we have shown...