A5046794685- Hepatitis B Virus Studies
- Parvovirus B19 Infection Studies
- Hepatitis C virus research
- Plant Virus Research Studies
- Polyomavirus and related diseases
- Toxoplasma gondii Research Studies
- Viral gastroenteritis research and epidemiology
- Drug Transport and Resistance Mechanisms
- Vibrio bacteria research studies
- Plant and Fungal Interactions Research
- HIV Research and Treatment
- Viral-associated cancers and disorders
- HIV/AIDS drug development and treatment
- Viral Infections and Vectors
- Animal Virus Infections Studies
- Bacteriophages and microbial interactions
- Biosensors and Analytical Detection
- Viral Infections and Outbreaks Research
- Virus-based gene therapy research
- Pneumocystis jirovecii pneumonia detection and treatment
- Hepatitis Viruses Studies and Epidemiology
- Blood donation and transfusion practices
- Viral Infections and Immunology Research
- Autoimmune Bullous Skin Diseases
- Malaria Research and Control
Institut National de la Transfusion Sanguine
2014-2022
Institut Pasteur
2011-2012
Université de Reims Champagne-Ardenne
2003-2009
Hôpital Maison Blanche
2008
École Polytechnique Fédérale de Lausanne
2007
Centre National de la Recherche Scientifique
2003-2006
Unit of Virus Host Cell Interactions
2006
Centre Hospitalier Universitaire de Reims
2003
The human skin is a complex ecosystem that hosts heterogeneous flora. Until recently, the diversity of cutaneous microbiota was mainly investigated for bacteria through culture based assays subsequently confirmed by molecular techniques. There are now many evidences viruses represent significant part flora as demonstrated asymptomatic carriage beta and gamma-human papillomaviruses on healthy skin. Furthermore, it has been recently suggested some representatives Polyomavirus genus might share...
High-throughput sequencing furnishes a large number of short sequence reads from uncloned DNA and has rapidly become major tool for identifying viruses in biological samples, particular when the target is undefined. In this study, we assessed analytical sensitivity pipeline detection samples based on either Roche-454 genome sequencer or Illumina analyzer platforms. We sequenced artificially spiked with wide range genomes composed single double-stranded RNA, including linear circular...
ABSTRACT Sulfadiazine, pyrimethamine, and atovaquone are widely used for the treatment of severe toxoplasmosis. Their in vitro activities have been almost exclusively demonstrated on laboratory strains belonging to genotype I. We determined these drugs against 17 Toxoplasma gondii various genotypes examined correlations among 50% inhibitory concentrations (IC 50 s), growth kinetics, strain genotypes, mutations drug target genes. Growth kinetics were THP-1 cell cultures using real-time PCR....
We have identified in a skin swab sample from healthy donor new virus that we named human gyrovirus (HGyV) because of its similarity to the chicken anemia (CAV), only previously known member Gyrovirus genus. In particular, this encodes homolog CAV apoptin, protein selectively induces apoptosis cancer cells. By PCR screening, HGyV was found 5 115 other nonlesional specimens but 0 92 bronchoalveolar lavages or nasopharyngeal aspirates and fecal samples.
While studying the virome of skin surface a patient with Merkel cell carcinoma (MCC) by using unbiased, high-throughput sequencing, we identified human polyomavirus nearly identical to 9, virus recently reported in blood and urine renal transplantion patients closely related African green monkey lymphotropic polyomavirus. Specific PCR analysis further this 2/8 MCC but only 1/111 controls without MCC. This was shed for ≥20 months index on spouse patient. These results provide information...
During a study of the fecal microbiomes from two healthy piglets using high-throughput sequencing (HTS), we identified viral genome containing an open reading frame encoding predicted polyprotein 2,133 amino acids. This novel displayed typical organization picornaviruses, three structural proteins (VP0, VP3, and VP1), followed by seven nonstructural (2A, 2B, 2C, 3A, 3B, 3C(pro), 3D(pol)). Given its particular relationship with Parechovirus, propose to name it "Pasivirus" for Parecho sister...
Several treatment failures have been reported for the of toxoplasmic encephalitis, chorioretinitis, and congenital toxoplasmosis. Recently we found three Toxoplasma gondii strains naturally resistant to sulfadiazine developed in vitro two strains, RH-RSDZ ME-49-RSDZ, by gradual pressure. In Plasmodium, common mechanisms drug resistance involve, among others, mutations and/or amplification within genes encoding therapeutic targets dhps dhfr ABC transporter family. To identify genotypic...
Seoul virus (SEOV) is a worldwide ratborne orthohantavirus. We describe an SEOV infection in adult returning to France from Kenya, followed by Guillain-Barré syndrome. confirmed PCR and sequencing. Although transmission might have occurred the epidemiologic information available not sufficient confirm that possibility.
Until recently, the method of choice to characterize viral diversity consisted in cloning PCR amplicons full-length genomes and Sanger-sequencing multiple clones. However, this is extremely laborious, time-consuming, low-throughput. Next generation short-read sequencing appears also limited by its inability directly sequence genomes. The MinION™ device recently developed Oxford Nanopore Technologies can be a promising alternative applying long-read single-molecule overall amplified products...
Abstract Usually transmitted via respiratory droplets, parvovirus B19 (B19V) can also be acquired by blood transfusion especially because of viral persistence, resistance to treatment procedures, and high load during the early infection phase. This is particularly problematic in immunocompromised or anemic patients where have a severe outcome. As B19V DNA was detected donations from South Brazil metagenomic survey performed Next‐Generation Sequencing, objective this retrospective study...
BACKGROUND False positivity in blood screening may cause unnecessary deferral of healthy donors and exacerbate shortages. An international multicenter study was conducted to estimate the frequency HCV HIV false seropositivity seven African countries (Burundi, Cameroon, Democratic Republic Congo, Madagascar, Mali, Mauritania, Niger). STUDY DESIGN AND METHODS Blood donations were tested for hepatitis C virus (HCV) human immunodeficiency (HIV) with rapid detection tests (RDTs), third‐generation...