A5032654308- Mitochondrial Function and Pathology
- Helicobacter pylori-related gastroenterology studies
- RNA and protein synthesis mechanisms
- DNA Repair Mechanisms
- Alzheimer's disease research and treatments
- Genetic and phenotypic traits in livestock
- Genetic Mapping and Diversity in Plants and Animals
- Meat and Animal Product Quality
- Animal Nutrition and Physiology
- ATP Synthase and ATPases Research
- Computational Drug Discovery Methods
- Cholinesterase and Neurodegenerative Diseases
- Histone Deacetylase Inhibitors Research
- Genomics and Phylogenetic Studies
- Cell Image Analysis Techniques
- Cellular transport and secretion
- Protein Kinase Regulation and GTPase Signaling
- Cancer, Hypoxia, and Metabolism
- Cell death mechanisms and regulation
- Biochemical Acid Research Studies
- Lipid metabolism and biosynthesis
- Metabolism, Diabetes, and Cancer
- Trace Elements in Health
- Sirtuins and Resveratrol in Medicine
- Microbial Inactivation Methods
Universidad de Zaragoza
2012-2023
Instituto de Investigación Sanitaria Aragón
2011-2020
Centro Médico Sanitas Zaragoza
2015
Instituto de Biocomputación y Física de Sistemas Complejos
2012
Stony Brook University
1999-2005
State University of New York
1999-2001
Consejo Superior de Investigaciones Científicas
1997
Instituto de Investigaciones Biomédicas Sols-Morreale
1997
Universidad Autónoma de Madrid
1997
Mitochondrial DNA polymerase gamma (pol gamma) is responsible for replication and repair of mtDNA mutated in individuals with genetic disorders such as chronic external ophthalmoplegia Alpers syndrome. pol also an adventitious target toxic side effects several antiviral compounds, mutation its proofreading exonuclease leads to accelerated aging mouse models. We have used a variety physical functional approaches study the interaction human catalytic subunit both wild-type accessory factor,...
Peptide sequences obtained from the accessory subunit of Xenopus laevis mitochondrial DNA (mtDNA) polymerase gamma (pol gamma) were used to clone cDNA encoding this protein. Amino-terminal sequencing protein indicated presence a 44-amino-acid targeting sequence, leaving predicted mature with 419 amino acids and molecular mass 47.3 kDa. This is associated larger, catalytic in preparations active mtDNA polymerase. The small exhibits homology its human, mouse, Drosophila counterparts....
Human Amylin, or islet amyloid polypeptide (hIAPP), is a small hormone secreted by pancreatic β-cells that forms aggregates under insulin deficiency metabolic conditions, and it constitutes pathological hallmark of type II diabetes mellitus. In patients, amylin abnormally increased, self-assembled into aggregates, ultimately contributes to the apoptotic death mechanisms are not completely understood. We have screened library approved drugs in order identify inhibitors aggregation could be...
Alzheimer's disease, characterized by deposits of amyloid β-peptide (Aβ), is the most common neurodegenerative but it still lacks a specific treatment. We have discovered five chemically unrelated inhibitors in vitro aggregation Aβ17–40 peptide screening two commercial chemical libraries. Four them (1–4) exhibit relatively low MCCs toward HeLa cells (17–184 μM). The usefulness compounds 1–4 to inhibit vivo Aβ1–42 has been demonstrated using fungi models, Saccharomyces cerevisiae and...
Success stories of citizen science projects widely demonstrate the value this open paradigm and encourage organizations to shift towards new ways doing research. While benefits for researchers are clear, outcomes individuals participating in these not easy assess. The wide spectrum volunteers collaborating greatly contributes difficulty evaluation projects' outcomes. Given strong links between many education, work we present an experience with hundreds students (aged 15–18) two different...
We have recently reported the crystal structure of accessory subunit mitochondrial DNA polymerase, pol γB, and identified a region protein involved in binding. The employed previous studies was presumed to be single-stranded, because it generated by single-sided PCR. Further characterization this indicated that, due strand transfer event during synthesis PCR, adopts double-stranded hairpin conformation under native conditions. used series double- single-stranded oligonucleotides different...
The function of the NaPiIIa renal sodium-phosphate transporter is regulated through a complex network interacting proteins. Several PDZ domain-containing proteins interact with its COOH terminus while small membrane protein MAP17 interacts NH(2) end. To elucidate MAP17, we identified using both bacterial and mammalian two-hybrid systems. proteins, including four NHERF as well NHE3, were found to bind MAP17. interactions further analyzed in opossum kidney (OK) cells. Expression alone had no...
Helicobacter pylori (Hp) infection is the main cause of peptic ulcer and gastric cancer. Hp eradication rates have fallen due to increasing bacterial resistance currently used broad-spectrum antimicrobials. We designed, synthesized, tested redox variants nitroethylene- 7-nitrobenzoxadiazole-based inhibitors essential protein flavodoxin. Derivatives 7-nitrobenzoxadiazole lead, carrying reduced forms nitro group and/or oxidized a sulfur atom, display high therapeutic indexes against several...
Previous studies have shown that the small subunit of Xenopus DNA polymerase γ (pol γB) acts as a processivity factor to stimulate 140 kDa catalytic human γ. A putative pol γB initially identified by analysis sequence had not been be functional, and appeared an incomplete clone. In this paper, we report cloning full-length mouse γB. Both proteins were expressed in their mature forms, without apparent mitochondrial localization signals, recombinant γA. Deletion indicated blocks conserved with...
Abstract Cytosolic phosphoenolpyruvate carboxykinase, PCK1, is one of the main regulatory enzymes gluconeogenesis and glyceroneogenesis. The substitution a single amino acid (Met139Leu) in PCK1 as consequence nucleotide polymorphism (SNP), c.A2456C, associated pig to negative phenotype characterized by reduced intramuscular fat content, enhanced backfat thickness lower meat quality. p.139L enzyme shows k cat values glyceroneogenic direction ones anaplerotic direction. Accordingly, expression...
Presenilin 1-associated protein (PSAP) was first identified as a that interacts with presenilin 1. It later reported PSAP is mitochondrial induces apoptosis when overexpressed in cultured cells. also known carrier homolog 1 (Mtch1). In this study, we show there are two proapoptotic isoforms generated by alternative splicing differ the length of hydrophilic loop located between predicted transmembrane domains. Using RT-PCR and Western blot assays, determined both expressed human rat tissues...
Background: An in silico mechanism-of-action analysis protocol was developed, comprising molecule bioactivity profiling, annotation of predicted targets with pathways and calculation enrichment factors to highlight more likely be implicated the studied phenotype. Results: The method applied a cytotoxicity phenotypic endpoint, enriched targets/pathways found statistically significant when compared 100 random datasets. Application on smaller apoptotic set (10 molecules) did not allowed obtain...
The cytosolic form of phosphoenolpyruvate carboxykinase (PCK1) plays a regulatory role in gluconeogenesis and glyceroneogenesis. the mitochondrial isoform (PCK2) remains unclear. We report partial purification kinetic functional characterization human PCK2. Kinetic properties enzyme are very similar to those enzyme. PCK2 has an absolute requirement for Mn2+ ions activity; Mg2+ reduce Km by about 60 fold. Its specificity constant is 100 fold larger oxaloacetate than suggesting that...
Bcl-Xl physically interacts with by cross-linking study (View interaction) Apoptosis is a type of cell death characterized the silent elimination unnecessary or damaged cells, which required for development and tissue homeostasis. Two major routes apoptosis have been described: extrinsic route initiated at surface mediated receptors, like Fas-CD95 [1]; intrinsic mitochondria [2], where many different signals are integrated final decision about fate made. Both linked in some cells Bid, member...
Summary The allelic frequencies of PRKAG 3 gene (the RN gene) have been investigated in several pig breeds. R 200 Q mutation appear only Hampshire pigs, whereas V 199 I is most abundant Iberian, P orco C elta or B izaro, and less breeds selected for muscularity as D uroc, L andrace ietrain. A thorough study phenotypic effects 1991 has performed a uroc × andrace‐ arge W hite cross. homozygous pigs show increased p H 24 values ham homogenates loin (0.14 0.16 units, respectively) compared to...