Protein N-homocysteinylation involves a post-translational modification by homocysteine (Hcy)-thiolactone. In humans, about 70% of circulating Hcy is N-linked to blood proteins, mostly hemoglobin and albumin. It was unclear what protein site(s) were prone attachment how affected function. Here we show that Lys525 predominant site in human serum albumin vitro vivo. We also the reactivity lysine residues, including Lys525, status Cys34. The disulfide forms albumin, albumin-Cys34-S-S-Cys...

<i>Objective:</i> The level of various specific biomarkers oxidative stress in plasma from schizophrenic patients, as well (the isoprostanes) urine patients was described. aim our present study to evaluate by oxidative/nitrative modifications proteins (by measuring the carbonyl groups and 3-nitrotyrosine proteins) with disorders control group. We also investigated low-molecular-weight thiols [glutathione (GSH), cysteine (CSH), cysteinylglycine (CGSH) homocysteine] obtained...

The mechanisms of oxidative stress in schizophrenic patients are not fully understood. In the present study, we investigated effect elevated level homocysteine (Hcys) on some parameters stress, namely thiobarbituric acid reactive substances (TBARS), an index lipid peroxidation plasma, carbonyl groups plasma proteins, as well amount 3-nitrotyrosine proteins isolated from patients. Patients hospitalised I and II Psychiatric Department Medical University Lodz, Poland were interviewed with...

Abstract Homocysteine (Hcy) and Hcy-thiolactone (HTL) affect fibrin clot properties are linked to cardiovascular disease. Factors that influence stroke not fully understood. To study sulfur-containing amino acid metabolites, lysis time (CLT) maximum absorbance (Abs max ) in relation stroke, we analyzed plasma urine from 191 patients (45.0% women, age 68 ± 12 years) 291 healthy individuals (59.7% 50 17 years). Plasma urinary levels of metabolites were significantly different compared...

Background This study investigated the effects of long term betaine supplementation on body composition, performance, and homocysteine thiolactone (HCTL) in experienced strength trained men.Methods Twenty-three subjects were matched for training experience (4.8 ± 2.3 years) fat percentage (BF%: 16.9 8.0%), randomly assigned to either a placebo (PL; n = 12) or group (BET; 11; 2.5 g/day), completed 6 week periodized program consisting 3 two-week micro-cycles. Bench press back squat volumes...

We have developed a simple, fast, accurate, and cheap method for the simultaneous determination of total cysteine, homocysteine, glutathione, N-acetylcysteine in brain homogenates based on reduction disulfide bonds by tris(2-carboxyethyl) phosphine, pre-column derivatization free thiol groups with 2-chloro-1-methylquinolinium tetrafluoroborate followed ion-pair reversed-phase high-performance liquid chromatography separation ultraviolet detection. The derivatives was achieved 10 min....

Cysteine (Cys) and glutathione (GSH) have been identified as key players in redox-based defense signaling under stress plants. Here, we describe a new high-performance liquid chromatography assay for the determination of four different forms Cys GSH, based on reduction disulfide bonds with tris(2-carboxyethyl)phosphine followed by derivatization 2-chloro-1-methylquinolinium tetrafluoroborate UV detection. The presented method allows facile total, reduced, free protein bound aminothiols...

An accurate, simple and sensitive method for simultaneous determination of total homocysteine (Hcy) cysteine (Cys) in human plasma has been developed validated. Hcy Cys play a major role metabolism cellular homeostasis. elevated level is considered an important risk factor or marker several diseases, particular cardiovascular disease, while involved variety functions such as protein synthesis, detoxification metabolism. The proposed analytical procedure consists reduction title thiol dimers...

Abstract Objectives No individual homocysteine (Hcy) metabolite has been studied as a risk marker for coronary artery disease ( CAD ). Our objective was to examine Hcy‐thiolactone, chemically reactive generated by methionyl‐ tRNA synthetase and cleared the kidney, predictor of incident acute myocardial infarction AMI ) in Western Norway B‐Vitamin Intervention Trial. Design Single centre, prospective double‐blind clinical intervention study, randomized 2 × factorial design. Subjects methods...

The report presents first high performance liquid chromatography coupled with ultraviolet detection (HPLC-UV) based method for the determination of homocysteine thiolactone (HTL) in human urine. Sample preparation procedure chiefly involves chloroform liquid-liquid extraction HTL and its re-extraction hydrochloric acid, followed by evaporation to dryness treatment residue 1-benzyl-2-chloropyridinium bromide perchloric acid. chromatographic separation obtained UV-absorbing 2-S-pyridinium...

The main source of bufadienolides is toad venom; however, plants such as members Kalanchoe Adans. (Crassulaceae) genus may also synthesize these bioactive substances.This the first study on antioxidant effects and cytotoxicity bufadienolide-rich fraction isolated from daigremontiana Raym.-Hamet & H. Perrier.The methanolic was extracted plant roots contained 0.48 mg bufadienolides/mg dry mass (11α,19-dihydroksytelocinobufagin, bersaldegenin-1-acetate, bersaldegenin-1,3,5-orthoacetate,...

The natural microbiota of the urinary tract includes Lactobacillus spp., which secrete molecules with antimicrobial properties and have antagonistic activity against many pathogens. This paper focuses on antibacterial effect strains isolated from urine clinical Proteus mirabilis kidney stones coexisting urolithiasis. study involved analyzing main secreted by Lactobacillus. In order to indicate agent had strongest effect, supernatants were made alkaline treated catalase high temperature. Both...

A new, highly reactive, and thiol specific derivatization reagent for liquid chromatographic analysis has been developed. The reagent, 2-chloro-1-methylquinolinium tetrafluoroborate, reacts instantaneously with hydrophilic thiols in water under mild conditions. 2-S-quinolinium derivatives, resulting from this reaction, are stable thioethers exhibiting well defined absorption maximum at 348 nm molar absorptivity coefficient about 2 × 10[4] Bald, E., Sypniewski, S., Drzewoski, J. Stepień,...