A5082923660- RNA and protein synthesis mechanisms
- Monoclonal and Polyclonal Antibodies Research
- vaccines and immunoinformatics approaches
- Cell Adhesion Molecules Research
- RNA modifications and cancer
- Psoriasis: Treatment and Pathogenesis
- Inflammatory Bowel Disease
- Polyomavirus and related diseases
- T-cell and B-cell Immunology
- Bacteriophages and microbial interactions
- Immune Cell Function and Interaction
- Atherosclerosis and Cardiovascular Diseases
- Immunodeficiency and Autoimmune Disorders
- Axon Guidance and Neuronal Signaling
- Prenatal Screening and Diagnostics
- RNA Research and Splicing
- Cancer Immunotherapy and Biomarkers
- SARS-CoV-2 and COVID-19 Research
- Whipple's Disease and Interleukins
- DNA Repair Mechanisms
- Reproductive System and Pregnancy
- Microscopic Colitis
- Fungal and yeast genetics research
- Biosimilars and Bioanalytical Methods
- COVID-19 Clinical Research Studies
AbbVie (United States)
2014-2025
BioPAL (United States)
2010
Abbott (United States)
2006-2010
Abbott (Germany)
2004
Baker Heart and Diabetes Institute
1995
Vrije Universiteit Amsterdam
1980-1984
Crohn's disease is associated with excess cytokine activity mediated by type 1 helper T (Th1) cells. Interleukin-12 a key that initiates Th1-mediated inflammatory responses.This double-blind trial evaluated the safety and efficacy of human monoclonal antibody against interleukin-12 (anti-interleukin-12) in 79 patients active disease. Patients were randomly assigned to receive seven weekly subcutaneous injections mg or 3 anti-interleukin-12 per kilogram body weight placebo, either four-week...
We present the sequence of 26S rRNA yeast Saccharomyces carlsbergensis as inferred from gene sequence. The molecule is 3393 nucleotides long and consists 48% G+C; 30 43 methyl groups can be located in Starting recently proposed structure E. coli 23S (see ref. 25) we constructed a secondary model for rRNA. This composed 7 domains closed by long-range base pairings n bacterial counterpart. Most show considerable conservation overall structure; unpaired regions extended homology base-paired...
Abstract The program death 1 (PD-1) receptor and its ligands, PD-1 ligand (PD-L)1 PD-L2, define a novel regulatory pathway with potential inhibitory effects on T, B, monocyte responses. In the present study, we show that human CD4+ T cells express PD-1, PD-L1, PD-L2 upon activation, Abs to can be agonists or antagonists of pathway. Under optimal conditions stimulation, ICOS but not CD28 costimulation prevented by engagement. IL-2 levels induced are critical in determining outcome Thus, low...
Sequences that comprise the 244-base-pair polyomavirus enhancer region are also required in cis for viral DNA replication (Tyndall et al., Nucleic Acids Res. 9:6231-6250, 1981). We have studied relationship between sequences activate and those enhance transcription two ways. One approach, recently described by de Villiers al. (Nature [London], 312:242-246, 1984), which was replaced with other or cellular transcriptional enhancers suggested an function is replication. The this paper, to...
We have determined the nucleotide sequence of part a cloned yeast ribosomal RNA operon extending from 5.8S gene downstream into 5' -terminal region 26S gene. mapped pertinent processing sites, viz. end rRNA and 3'ends its immediate precursor, 7S RNA. At 3' we find UCGUUU which is very similar to type I consensus UCAUUA/U present at ends 17S, as well 18S precursor in yeast. thirteen nucleotides homologous II termini both 17S These findings further support suggestion put forward earlier (G.M....
P-selectin glycoprotein ligand-1 (PSGL-1) is the high affinity counter-receptor for on myeloid cells (Sako, D., Chang, X. J., Barone, K. M., Vachino, G., White, H. Shaw, Veldman, G. Bean, Ahern, T. Furie, B., Cumming, D. A., and Larsen, R.(1993) Cell 75, 1179-1186). Here we demonstrate that PSGL-1 also widely distributed T- B-lymphocytic tumor cell lines, resting peripheral blood T B cells, stimulated intestinal intraepithelial lymphocyte (IEL) lines. However, majority of PSGL-1-positive...
The sequences of the nontranscribed spacers (NTS) cloned ribosomal DNA (rDNA) units from both Saccharomyces cerevisiae and carlsbergensis were determined. NTS species found to be 93% homologous. major disparities comprise different frequencies reiteration short tracts six sixteen basepairs. Most these reiterations are within 1100 basepairs long between 3'-ends 26S 5S rRNA (NTS1). starts 37S pre-rRNA (NTS2) comprises about 1250 first 800 NTS2 (adjacent gene) virtually identical in strains...
Modified polyomavirus genomes that individually encode the large and small T proteins were constructed by exchanging restriction endonuclease fragments between cDNA copies of respective mRNAs cloned genomic DNA. The efficacies new constructs, middle protein gene described previously (R. Treisman , U. Novak, J. Favaloro R. Kamen Nature [London] 292:595-600, 1981), demonstrated with simian virus 40 (SV40)-polyomavirus recombinants in which part or all SV40 late region was replaced modified...
Control of the ongoing SARS-CoV-2 pandemic is endangered by emergence viral variants with increased transmission efficiency, resistance to marketed therapeutic antibodies, and reduced sensitivity vaccine-induced immunity. Here, we screen B cells from COVID-19 donors identify P5C3, a highly potent broadly neutralizing monoclonal antibody picomolar activity against all concern (VOCs) identified date. Structural characterization P5C3 Fab in complex spike demonstrates defined large buried...
Initial rolling of circulating neutrophils on a blood vessel wall prior to adhesion and transmigration damaged tissue is dependent upon P-selectin expressed endothelial cells its specific neutrophil receptor, the glycoprotein ligand-1 (PSGL-1). Pretreatment neutrophils, HL60 cells, or recombinant fucosylated soluble form PSGL-1 (sPSGL-1.T7) with cobra venom metalloproteinase, mocarhagin, completely abolished binding purified in time-dependent EDTA- diisopropyl fluorophosphate-inhibitable...
The DNA sequence of the intergenic region between 17S and 5.8S rRNA genes ribosomal RNA operon in yeast has been determined. In this 37S precursor is specifically cleaved at a number sites course maturation process. exact position these processing established by analysis terminal fragments respective species. There appears to be no significant complementarity sequences surrounding two termini 18S secondary nor those mature rRNA. This finding implies that not directed double-strand specific...
Journal Article The transcription termination site of the ribosomal RNA operon in yeast Get access Geertruida M. Veldman, Veldman Biochemisch Laboratorium, Vrije Universiteitde Boelelaan 1083, 1081 HV Amsterdam, Netherlands Search for other works by this author on: Oxford Academic PubMed Google Scholar Jacobus Klootwijk, Klootwijk Petrus de jonge, jonge Robert J. Leer, Leer Rudi Planta Nucleic Acids Research, Volume 8, Issue 22, 25 November 1980, Pages 5179–5192,...
Four antibodies that inhibit interleukin (IL)-23 are approved for the treatment of moderate-to-severe plaque psoriasis. Here, we present non-clinical data comparing ustekinumab, guselkumab, tildrakizumab and risankizumab with regard to thermostability, IL-23 binding affinity, inhibitory-binding mode, in vitro potency vivo efficacy. Risankizumab guselkumab exhibited 5-fold higher affinity showed more potent inhibition signaling than ustekinumab tildrakizumab. completely blocked IL-23Rα as...
Immunogenic responses to biotherapeutics often lead termination of their development because the resulting anti-drug-antibodies (ADA) can negatively impact pharmacology, safety, and efficacy. To mitigate ADA risks, in vitro risk assessment assays non-clinical settings are essential enhance safety efficacy protein-based therapeutics. This study aimed develop validate a human immunogenicity T cell proliferation assay. However, there is lack comprehensive guidelines for managing product-related...
Immunotherapy targeting amyloid-β peptide is under active clinical investigation for treatment of Alzheimer's disease (AD). Among the hypotheses being investigated impact on outcome are preferred epitope or conformation to target treatment, and mechanism action underlying immunotherapy. Bapineuzumab (humanized 3D6), a neo-epitope specific antibody recognizing amyloid-β1-5 with strong preference an exposed Asp residue at N-terminus peptide, has undergone advanced testing AD.To gain further...
Fibrotic diseases, such as idiopathic pulmonary fibrosis (IPF) and systemic scleroderma (SSc), are commonly associated with high morbidity mortality, thereby representing a significant unmet medical need. Interleukin 11 (IL11)-mediated cell activation has been identified central mechanism for promoting downstream of TGFβ. IL11 signaling recently reported to promote fibroblast-to-myofibroblast transition, thus leading various pro-fibrotic phenotypic changes. We confirmed increased mRNA...
Sequences that comprise the 244-base-pair polyomavirus enhancer region are also required in cis for viral DNA replication (Tyndall et al., Nucleic Acids Res. 9:6231-6250, 1981). We have studied relationship between sequences activate and those enhance transcription two ways. One approach, recently described by de Villiers al. (Nature [London], 312:242-246, 1984), which was replaced with other or cellular transcriptional enhancers suggested an function is replication. The this paper, to...
Immunogenicity is a major challenge in drug development and patient care. Currently, most efforts are dedicated to the elimination of unwanted immune responses through T-cell epitope prediction protein engineering. However, because it unlikely that this approach will lead complete eradication immunogenicity, we propose quantitative systems pharmacology models should be developed predict manage immunogenicity. The potential impact such mechanistic model-based precedented by applications...