A5022379888- Inflammatory Bowel Disease
- Microscopic Colitis
- Gut microbiota and health
- Immune Cell Function and Interaction
- Neuropeptides and Animal Physiology
- Immunodeficiency and Autoimmune Disorders
- Receptor Mechanisms and Signaling
- T-cell and B-cell Immunology
- IL-33, ST2, and ILC Pathways
- Eosinophilic Esophagitis
- Helicobacter pylori-related gastroenterology studies
- Systemic Lupus Erythematosus Research
- Psoriasis: Treatment and Pathogenesis
- Clostridium difficile and Clostridium perfringens research
- Celiac Disease Research and Management
- Diet and metabolism studies
- Hypothalamic control of reproductive hormones
- Gastrointestinal motility and disorders
- HIV Research and Treatment
- Gastrointestinal disorders and treatments
- Metabolism and Genetic Disorders
- Esophageal Cancer Research and Treatment
- Autoimmune and Inflammatory Disorders
- Mycobacterium research and diagnosis
- Immunotherapy and Immune Responses
University of Nebraska Medical Center
2022-2024
University of Alabama at Birmingham
2013-2023
University of Nebraska at Omaha
2023
Omaha VA Medical Center
2022
Birmingham VA Medical Center
2020-2021
Imelda Hospital
2021
National Institutes of Health
2002-2014
National Institute of Allergy and Infectious Diseases
2004-2011
Oklahoma Foundation for Digestive Research
2011
Mayo Clinic in Arizona
2008
Abstract Background To understand the relationship between our bacterial microbiome and health, it is essential to define in absence of disease. The digestive tract includes diverse habitats hosts human body's greatest density. We describe community composition ten sites from more than 200 normal adults enrolled Human Microbiome Project, metagenomically determined metabolic potentials four representative sites. Results microbiota these formed groups based on similar compositions: buccal...
Crohn's disease is associated with excess cytokine activity mediated by type 1 helper T (Th1) cells. Interleukin-12 a key that initiates Th1-mediated inflammatory responses.This double-blind trial evaluated the safety and efficacy of human monoclonal antibody against interleukin-12 (anti-interleukin-12) in 79 patients active disease. Patients were randomly assigned to receive seven weekly subcutaneous injections mg or 3 anti-interleukin-12 per kilogram body weight placebo, either four-week...
While Crohn disease (CD) has been clearly identified as a Th1 inflammation, the immunopathogenesis of its counterpart inflammatory bowel disease, ulcerative colitis (UC), remains enigmatic. Here we show that lamina propria T (LPT) cells from UC patients produce significantly greater amounts IL-13 (and IL-5) than control and little IFN-γ, whereas comparable CD large IFN-γ small IL-13. We then stimulation LPT bearing an NK marker (CD161) with anti-CD2/anti-CD28 or B expressing transfected CD1d...
The renin-angiotensin system plays a critical role in sodium and fluid homeostasis. Genetic or acquired alterations the expression of components this are strongly implicated pathogenesis hypertension. To specifically examine physiological genetic functions type 1A receptor for angiotensin II, we have disrupted mouse gene encoding embryonic stem cells by targeting. Agtr1A(-/-) mice were born expected numbers, histomorphology their kidneys, heart, vasculature was normal. AT1 receptor-specific...
Human immunodeficiency virus (HIV) persists in peripheral blood mononuclear cells despite sustained, undetectable plasma viremia resulting from long-term antiretroviral therapy. However, the source of persistent HIV such infected individuals remains unclear. Given recent data suggesting high levels viral replication and profound depletion CD4(+) T gut-associated lymphoid tissue (GALT) animals with simian HIV-infected humans, we sought to determine level cell as well degree extent persistence...
Interleukin (IL)-12p70 and IL-23 are key T helper-1 (TH1) cytokines that drive the inflammation seen in numerous models of intestinal inflammation. These molecules contain an identical p40 chain is bound to a p35 IL-12 p19 IL-23, making both potentially susceptible modulation by anti-IL-12p40 monoclonal antibody (mAb).In present study, we sought determine whether active Crohn's disease (CD) associated with increased synthesis these patients treated mAb down-regulate as well IL-12p70 previous...
The renin-angiotensin system (RAS) is a key regulator of vascular tone and blood pressure. In addition, angiotensin II also has number cellular effects that may contribute to disease pathogenesis. Using Agtr1a–/– mice, which lack AT1A receptors for II, we have identified novel function the RAS modulate immune system. We find acting through type 1 (AT1) on cells, triggers proliferation splenic lymphocytes. These actions vigor alloimmune responses. Within lymphoid organs, sufficient components...
<h3>Objective</h3> Ulcerative colitis is associated with increased interleukin 13 (IL-13) production by natural killer T cells. Taking advantage of the inhibitory actions interferon β on IL-13 expression, this proof-of-concept study aimed to show that decreasing clinical improvement ulcerative symptoms. <h3>Design</h3> Open-label interventional drug trial. <h3>Setting</h3> Outpatient research hospital. <h3>Patients</h3> Adult patients active (Short Clinical Colitis Activity Index (SCCAI)≥5)....
Objective Previous studies have shown that ulcerative colitis (UC) is associated with the presence of lamina propria non-invariant (Type II) NKT cells producing IL-13 and mediating epithelial cell cytotoxicity. Here we sought to define antigen(s) stimulating quantitate these in UC propria. Design Detection Type II mononuclear (LPMC) lyso-sulfatide loaded tetramer quantum dot-based flow cytometry staining. Culture LPMCs glycolipid determine sulfatide induction cytotoxicity, production...
We synthesized PYY-(1–36) (nonselective between Y 1 and 2 receptor subtype agonists), [Pro 34 ]PYY (selective for ), PYY-(3–36) ) to determine whether solution conformation plays a role in selectivity. The three peptides exhibited the expected specificities displacing labeled from cells transfected with receptors (dissociation constants = 0.42, 0.21, 1,050 nM, respectively) 0.03, 710, 0.11 PYY-(1–36), ]PYY, PYY-(3–36). Sedimentation equilibrium analyses revealed that PYY analogs were 80–90%...
While Crohn disease (CD) has been clearly identified as a Th1 inflammation, the immunopathogenesis of its counterpart inflammatory bowel disease, ulcerative colitis (UC), remains enigmatic. Here we show that lamina propria T (LPT) cells from UC patients produce significantly greater amounts IL-13 (and IL-5) than control and little IFN-γ, whereas comparable CD large IFN-γ small IL-13. We then stimulation LPT bearing an NK marker (CD161) with anti-CD2/anti-CD28 or B expressing transfected CD1d...
Activation by multi-epitope peptide plus mTOR inhibition depletes flagellin-reactive CD4 + T cells and prevents intestinal inflammation.
Whether or not populations diverge with respect to the genetic contribution risk of specific complex diseases is relevant understanding evolution susceptibility and origins health disparities. Here, we describe a large-scale whole-genome sequencing study inflammatory bowel disease encompassing 1,774 affected individuals 1,644 healthy control Americans African ancestry (African Americans). Although no new loci for are discovered at genome-wide significance levels, identify numerous instances...