A5084861556- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- SARS-CoV-2 detection and testing
- Asthma and respiratory diseases
- Immune Cell Function and Interaction
- Long-Term Effects of COVID-19
- Allergic Rhinitis and Sensitization
- T-cell and B-cell Immunology
- Urticaria and Related Conditions
- vaccines and immunoinformatics approaches
- Viral Infections and Immunology Research
- Virus-based gene therapy research
- Immune cells in cancer
- Cancer Immunotherapy and Biomarkers
- Drug-Induced Adverse Reactions
- COVID-19 epidemiological studies
- Trace Elements in Health
- Viral gastroenteritis research and epidemiology
- Food Allergy and Anaphylaxis Research
- CAR-T cell therapy research
- Vitamin D Research Studies
- Bacteriophages and microbial interactions
- COVID-19 Impact on Reproduction
- Pediatric health and respiratory diseases
- Immunotherapy and Immune Responses
SIB Swiss Institute of Bioinformatics
2024
University of Lausanne
2012-2024
Weatherford College
2024
University Hospital of Lausanne
2008-2022
Weizmann Institute of Science
2009
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)-specific antibody responses to the spike (S) protein monomer, S native trimeric form, or nucleocapsid (N) proteins were evaluated in cohorts of individuals with infection (n = 93) and enrolled a postinfection seroprevalence population study 578) Switzerland. Commercial assays specific for S1 N protein, within newly developed Luminex assay using trimer found be equally sensitive detection acute-infection-phase samples....
In the present study, we have investigated functional profile of CD4 T cells from patients with common variable immunodeficiency (CVID), including production cytokines and proliferation in response to bacteria virus-derived antigens. We show that impairment cells, reduced capacity proliferate produce IFN-γ IL-2, was restricted bacteria-specific not virus-specific cells. High levels endotoxins were found plasma CVID, suggesting cell dysfunction might be caused by bacterial translocation. Of...
The detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)-specific antibodies in the serum an individual indicates previous infection or vaccination. However, it provides limited insight into protective nature this immune response. Neutralizing recognizing viral spike protein are more revealing, yet their measurement traditionally requires virus- and cell-based systems that costly, time-consuming, inflexible, potentially biohazardous. Here, we present a cell-free...
There are limited comparative data on the durability of neutralizing antibody (nAb) responses elicited by messenger RNA (mRNA) vaccines against SARS-CoV-2 variants concern (VOCs) in immunocompromised patients and healthy controls.
Expression of co-inhibitory molecules is generally associated with T-cell dysfunction in chronic viral infections such as HIV or HCV. However, their relative contribution the impairment remains unclear. In present study, we have evaluated impact expression 2B4, PD-1 and CD160 on functions CD8 T-cells specific to influenza, EBV CMV. We show that populations expressing CD160, but not PD-1, had reduced proliferation capacity perforin expression, thus indicating functional CD160+ T cells may be...
Control of the ongoing SARS-CoV-2 pandemic is endangered by emergence viral variants with increased transmission efficiency, resistance to marketed therapeutic antibodies, and reduced sensitivity vaccine-induced immunity. Here, we screen B cells from COVID-19 donors identify P5C3, a highly potent broadly neutralizing monoclonal antibody picomolar activity against all concern (VOCs) identified date. Structural characterization P5C3 Fab in complex spike demonstrates defined large buried...
Abstract The SARS-CoV-2 Omicron variant has very high levels of transmission, is resistant to neutralization by authorized therapeutic human monoclonal antibodies (mAb) and less sensitive vaccine-mediated immunity. To provide additional therapies against Omicron, we isolated a mAb named P2G3 from previously infected vaccinated donor showed that it picomolar-range neutralizing activity BA.1, BA.1.1, BA.2 all other variants tested. We solved the structure Fab in complex with spike using...
Introduction . Preclinical and clinical evidences for a role of oral probiotics in the management allergic diseases are emerging. Aim We aimed at testing immunomodulatory effects intranasal versus intragastric administration Lactobacillus paracasei NCC2461 mouse model airway inflammation specificity different by comparing L. to plantarum NCC1107. Methods or NCC1107 strains were administered either intragastrically (NCC2461) intranasally (NCC2461 NCC1107) OVA-sensitized mice challenged with...
Abstract To better understand the development of SARS-CoV-2-specific immunity over time, a detailed evaluation humoral and cellular responses is required. Here, we characterize anti-Spike (S) IgA IgG in representative population-based cohort 431 SARS-CoV-2-infected individuals up to 217 days after diagnosis, demonstrating that 85% develop maintain anti-S responses. In subsample 64 participants, further assess anti-Nucleocapsid (N) IgG, neutralizing antibody activity, T cell Membrane (M), N,...
Synthetic contiguous overlapping peptides (COPs) may represent an alternative to allergen extracts or recombinant allergens for specific immunotherapy. In combination, COPs encompass the entire sequence, providing all potential T cell epitopes, while preventing IgE conformational epitopes of native allergen.Individual were derived from sequence Bet v 1, major birch pollen, and its known crystal structure, designed avoid binding. Three sets tested in vitro competition ELISA basophil...
Summary Background Regulatory T cells (Tregs) are key players in controlling the development of airway inflammation. However, their role mechanisms leading to tolerance established allergic asthma is unclear. Objective To examine Tregs induction a murine model asthma. Methods Ovalbumin (OVA) sensitized asthmatic mice were depleted or not CD25 + by anti‐CD25 PC61 monoclonal antibody (mAb) before intranasal treatment (INT) with OVA, then challenged OVA aerosol. further evaluate respective...
Abstract Background Seroprevalence and the proportion of people with neutralizing activity (functional immunity) against SARS-CoV-2 variants were high in early 2022. In this prospective, population- based, multi-region cohort study, we assessed development functional hybrid immunity (induced by vaccination infection) general population during period incidence infections Omicron variants. Methods We randomly selected individuals aged ≥16 years from southern (n = 739) north-eastern 964)...
Classical antagonistic antibodies (Abs) targeting PD-1, such as pembrolizumab and nivolumab, act through blockade of the PD-1-PDL-1 interaction. Here, we have identified novel anti-PD-1 Abs not blocking The nonblocking recognize epitopes on PD-1 located opposing face PDL-1 interaction overlap with a newly evolutionarily conserved patch. These predominantly CD28 coreceptor. Importantly, combination synergize in functional recovery antigen-specific exhausted CD8 T cells. Interestingly,...
Abstract We have determined SARS-CoV-2-specific antibody responses in a cohort of 96 individuals with acute infection and 578 enrolled seroprevalence population study Switzerland including three groups, i.e. subjects previous RT-PCR confirmed SARS-CoV-2 infections (n=90), ‘positive patient contacts’ (n=177) ‘random selected subjects’ (n=311). specific to the Spike (S), monomeric native trimeric forms, and/or nucleocapsid (N) proteins were equally sensitive phase. Interestingly, as compared...
Functional immunity (defined here as serum neutralising capacity) critically contributes to conferring protection against SARS-CoV-2 infection and severe COVID-19. This cross-sectional analysis of a prospective, population-based cohort study included 1,894 randomly-selected 16 99-year-old participants from two Swiss cantons in March 2022. Of these, 97.6% (95% CI: 96.8-98.2%) had anti-spike IgG antibodies, capacity was respectively observed for 94%, 92% 88% wild-type SARS-CoV-2, Delta Omicron...
Adenovirus serotype 5 (Ad5) vectors and specific neutralizing antibodies (NAbs) generate immune complexes (ICs) which are potent inducers of dendritic cell (DC) maturation. Here we show that ICs generated with rare Ad vector serotypes, such as Ad26 Ad35, lead candidates in HIV vaccine development, poor DC maturation their potency inducing strongly correlated the number Toll-like receptor 9 (TLR9)-agonist motifs present vector's genome. In addition, showed antihexon but not antifiber...
A better understanding of the immunological markers associated with long-lasting immune responses to SARS-CoV-2 infection is paramount importance. In present study, we characterized SARS-CoV-2-specific humoral in hospitalized (ICU and non-ICU) non-hospitalized individuals at six months post-onset symptoms (POS) (N = 95). We showed that proportion detectable anti-SARS-CoV-2 IgG or neutralizing (NAb) titers antibodies were significantly reduced individuals, compared ICU- non-ICU-hospitalized 6...
New onsets of chronic urticaria (CU) have been reported after repeated immunizations, mainly with the Moderna mRNA-1273 vaccine (Spikevax). This study aims to evaluate patients CU COVID-19 mRNA vaccination. The contribution SARS-Cov2 infection, atopy and IgE against was analyzed. We monitored features who developed vaccination through two surveys conducted in 2022 2023. Fifty individuals underwent blood tests, their results were compared without a history (N = 135). presence anti-vaccine...
In these times of COVID-19 pandemic, concern has been raised about the potential effects SARS-CoV-2 infection on immunocompromised patients, particularly those receiving B-cell depleting agents and having therefore a severely depressed humoral response. Convalescent plasma can be therapeutic option for patients. Understanding underlying mechanisms convalescent is crucial to optimize such approach. Here, we describe patient who was deeply immunosuppressed following rituximab (anti-CD20...
Abstract The SARS-CoV-2 Omicron variant exhibits very high levels of transmission, pronounced resistance to authorized therapeutic human monoclonal antibodies and reduced sensitivity vaccine-induced immunity. Here we describe P2G3, a antibody (mAb) isolated from previously infected vaccinated donor, which displays picomolar-range neutralizing activity against BA.1, BA.1.1, BA.2 all other current variants, is thus markedly more potent than or clinically advanced anti-SARS-CoV-2 mAbs....
Between March 2021 and February 2022, SARS-CoV-2 neutralizing antibodies dynamics was investigated in a prospective observational study 903 healthcare workers of hospital Switzerland. A surrogate neutralization assay measuring the competitive inhibition angiotensin converting enzyme 2 (ACE2) binding to spike protein (S) wild type virus five variants concern (Alpha, Beta, Gamma, Delta, Omicron) used. We observed broad distribution activity among participants substantial differences titers...
IntroductionSome micronutrients have key roles in immune defence, including mucosal defence mechanisms and immunoglobulin production. Altered micronutrient status has been linked with COVID-19 infection disease severity. We assessed the associations of selected circulating anti-SARS-CoV-2 IgG IgA seropositivity Swiss community using early pandemic data.MethodsCase-control study comparing first PCR-confirmed symptomatic cases Vaud Canton (May to June 2020, n = 199) controls (random population...
Abstract The detection of SARS-CoV-2-specific antibodies in the serum an individual indicates prior infection or vaccination. However, it provides limited insight into protective nature this immune response. Neutralizing recognizing viral Spike are far more revealing, yet their measurement traditionally requires virus- and cell-based systems that costly, time-consuming, poorly flexible potentially biohazardous. Here we present a cell-free quantitative neutralization assay based on...