A5020540850- Immune Cell Function and Interaction
- Systemic Lupus Erythematosus Research
- T-cell and B-cell Immunology
- Immunodeficiency and Autoimmune Disorders
- Drug-Induced Adverse Reactions
- Vasculitis and related conditions
- Otitis Media and Relapsing Polychondritis
- Food Allergy and Anaphylaxis Research
- Pneumocystis jirovecii pneumonia detection and treatment
- COVID-19 Clinical Research Studies
- SARS-CoV-2 and COVID-19 Research
- Long-Term Effects of COVID-19
- Allergic Rhinitis and Sensitization
- Cancer Immunotherapy and Biomarkers
- Urticaria and Related Conditions
- Liver Disease Diagnosis and Treatment
- Monoclonal and Polyclonal Antibodies Research
- Asthma and respiratory diseases
- Liver Diseases and Immunity
- Immune cells in cancer
- Antibiotics Pharmacokinetics and Efficacy
- Chronic Kidney Disease and Diabetes
- CAR-T cell therapy research
- Renal Diseases and Glomerulopathies
- Atherosclerosis and Cardiovascular Diseases
University of Lausanne
2014-2025
University Hospital of Lausanne
2015-2025
Hôpital de l'enfance
2021
Harvard University
2015-2019
Beth Israel Deaconess Medical Center
2015-2019
Hôpital Orthopédique de la Suisse Romande
2012-2018
Harvard Affiliated Emergency Medicine Residency
2015
Abstract The objective of the present study was to identify biological signatures severe coronavirus disease 2019 (COVID-19) predictive admission in intensive care unit (ICU). Over 170 immunological markers were investigated a ‘discovery’ cohort (n = 98 patients) Lausanne University Hospital (LUH-1). Here we report that 13 out 49 cytokines significantly associated with ICU three cohorts ( P < 0.05 0.001), while cellular lacked power discriminating between and non-ICU patients. cytokine...
Abstract HCV infection has a severe course of disease in HIV/HCV co‐infection and liver transplant recipients. However, the mechanisms involved remain unclear. Here, we evaluated functional profiles HCV‐specific T‐cell responses 86 mono‐infected patients, 48 co‐infected patients 42 IFN‐γ IL‐2 production ability CD4 CD8 T cells to proliferate were assessed after stimulation with HCV‐derived peptides. We observed that polyfunctional presence proliferating single IL‐2‐, dual IL‐2/IFN‐γ...
In the present study, we have investigated functional profile of CD4 T cells from patients with common variable immunodeficiency (CVID), including production cytokines and proliferation in response to bacteria virus-derived antigens. We show that impairment cells, reduced capacity proliferate produce IFN-γ IL-2, was restricted bacteria-specific not virus-specific cells. High levels endotoxins were found plasma CVID, suggesting cell dysfunction might be caused by bacterial translocation. Of...
Systemic lupus erythematosus (SLE) is characterized by dysregulated humoral immunity, leading to the generation of autoreactive B cells that can differentiate both within and outside lymph node (LN) follicles. Here, we employed spatial transcriptomics multiplex imaging investigate follicular immune landscaping in situ transcriptomic profile LNs from SLE individuals. Our analysis revealed robust type I IFN plasma cell signatures compared reactive, control Cell deconvolution T subsets are...
Inducible cAMP early repressor (ICER) has been described as a transcriptional isoform of the response element modulator (CREM). Here we report that ICER is predominantly expressed in Th17 cells through IL-6-STAT3 pathway and binds to Il17a promoter, where it facilitates accumulation canonical enhancer RORγt. In vitro differentiation from naive ICER/CREM-deficient CD4+ T impaired but can be rescued by forced overexpression ICER. Consistent with role autoimmune inflammatory diseases, B6.lpr...
Systemic lupus erythematosus (SLE) is a devastating autoimmune disease in which hyperactive T cells play critical role. Understanding molecular mechanisms underlying the cell hyperactivity will lead to identification of specific therapeutic targets. Serine/arginine-rich splicing factor 1 (SRSF1) an essential RNA-binding protein that controls posttranscriptional gene expression. We have demonstrated SRSF1 levels are aberrantly decreased from patients with SLE and they correlate severe...
Effector CD8+ T cell function is impaired in systemic lupus erythematosus (SLE) and associated with a compromised ability to fight infections. Signaling lymphocytic activation molecule family member 7 (SLAMF7) engagement has been shown enhance natural killer degranulation. This study was undertaken characterize the expression of SLAMF7 on subsets isolated from peripheral blood SLE patients healthy subjects.CD8+ subset distribution, expression, cytolytic enzymes (perforin, granzyme A [GzmA],...
Expression of co-inhibitory molecules is generally associated with T-cell dysfunction in chronic viral infections such as HIV or HCV. However, their relative contribution the impairment remains unclear. In present study, we have evaluated impact expression 2B4, PD-1 and CD160 on functions CD8 T-cells specific to influenza, EBV CMV. We show that populations expressing CD160, but not PD-1, had reduced proliferation capacity perforin expression, thus indicating functional CD160+ T cells may be...
Imbalanced cytokine production by T cells characterizes both patients with systemic lupus erythematosus (SLE) and lupus-prone mice contributes to immune dysregulation. This study was undertaken further investigate in detail the of interleukin-2 (IL-2), interferon-γ (IFNγ), IL-4, IL-17A CD4+ cell subsets healthy subjects SLE, signaling response exogenous IL-2.Cytokine differentiated assessed intracellular staining following stimulation phorbol myristate acetate ionomycin enzyme-linked...
Engagement of signaling lymphocytic activation molecule family member 4 (SLAMF4; CD244, 2B4) by its ligand SLAMF2 (CD48) modulates the function and expansion both natural killer cells a subset cytotoxic CD8+ T cells. Because cytotoxicity lymphocytes isolated from patients with systemic lupus erythematosus (SLE) is known to be impaired, aim this study was assess whether expression checkpoint regulator SLAMF4 are altered on SLE.The healthy donors SLE determined quantitative polymerase chain...
Systemic lupus erythematosus (SLE) is a multisystem autoimmune disease characterized by multiple cellular and molecular dysfunctions of the innate adaptive immunity. Cytotoxic function NK cells compromised in patients with SLE. Herein, we phenotypic alterations SLE comprehensive manner to further delineate mechanisms underlying cytotoxic dysfunction identify novel potential therapeutic targets. Therefore, examined PBMC from matched healthy controls single-cell mass cytometry assess phenotype...
Systemic lupus erythematosus (SLE) in males is rare and poorly understood. Thus, still little known about sex differences SLE. We set out to identify regarding clinical manifestations as well renal cardiovascular outcomes of analyzed patient data from the Swiss SLE Cohort Study. Cumulative according updated American College Rheumatology criteria were recorded at inclusion. Cardiovascular events within Lupus International Collaborating Clinics/American Damage Index (SLICC-SDI). Renal failure...
Abstract Hepatitis C virus (HCV)‐specific CD4 + and CD8 T cell responses were investigated using a panel of 728 overlapping peptides spanning the whole HCV genome in 47 mono‐infected 26 HIV/HCV co‐infected individuals IFN‐γ ELISPOT assay flow cytometry. The frequency HCV‐specific was similar (∼40%) both groups, but breadth tended to be reduced individuals. Of interest, 23 new HCV‐derived epitopes identified, detected overall proportion responses. A tendency towards dominant response...
Significance Systemic lupus erythematosus (SLE) is characterized by compromised IL-2 production and regulatory T-cell function. Studies in human SLE murine models report that replenishment ameliorates clinical manifestations. Here we show engagement of signaling lymphocytic activation molecule family 3 (SLAMF3), a coregulatory receptor T cells, restores the sensitivity CD4 + cells to IL-2, increasing their response exogenous via up-regulation IL-2Rα subunit. Moreover, naïve with monoclonal...
A 22-year-old male with a typical history of pauci-symptomatic COVID-19 3 weeks earlier, confirmed by positive serology for SARS-CoV-2 (IgG), was admitted to the intensive care unit because severe myocarditis refractory cardiogenic shock that required extracorporeal life support. Due clinical presentation suggestive Kawasaki-like disease coronary aneurysm and systemic inflammation, intravenous immunoglobulins were administered in combination tocilizumab. The initial course favourable these...
Genome-wide linkage analysis studies (GWAS) in systemic lupus erythematosus (SLE) identified the 1q23 region on human chromosome 1, containing Signaling Lymphocytic Activation Molecule Family (SLAMF) cluster of genes, as a susceptibility locus. The SLAMF molecules (SLAMF1-7) are immunoregulatory receptors expressed predominantly hematopoietic cells. cells adaptive immune system is aberrant SLE and dysregulated expression certain has been reported. We examined SLAMF1-7 peripheral blood T...
Dear editor, The rapidly expanding coronavirus disease 2019 (COVID-19) is a global health challenge and source of anxiety amplified by the constant news stream. The mortality rate from COVID-19 difficult to establish given heterogeneity reports confounding factors such as difference in healthcare systems around world. occurrence severe acute respiratory distress syndrome (ARDS) are increased elderly patients with underlying conditions cardiovascular chronic conditions. To date, neither...
Objectives Natural Killer (NK) cell dysfunction contributes to systemic lupus erythematosus (SLE) pathogenesis, but the underlying mechanisms remain poorly understood. This study investigates immunometabolic alterations in NK cells from SLE patients and explores therapeutic strategies for their restoration. Methods We characterized mitochondrial structure function peripheral blood of healthy controls using flow cytometry, electron microscopy, proteomics. Key mitophagy-related gene...
Objective Signaling lymphocytic activation molecule family member 1 ( SLAMF 1) homophilic interactions promote immunoglobulin production and T cell–B cell cross‐talk. is overexpressed on B cells in patients with systemic lupus erythematosus SLE ). This study was undertaken to determine the role of monoclonal antibody mA b) modulating interaction activation. Methods Anti‐IgM–prestimulated naive or total from either healthy donors were cocultured autologous under CD 3/ 28 stimulation, presence...