A5100667110- Coccidia and coccidiosis research
- Helminth infection and control
- Parasitic Infections and Diagnostics
- Veterinary medicine and infectious diseases
- Parasites and Host Interactions
- Animal Nutrition and Physiology
- Toxoplasma gondii Research Studies
- Parasite Biology and Host Interactions
- Herpesvirus Infections and Treatments
- Parasitic Diseases Research and Treatment
- Peptidase Inhibition and Analysis
- Parasitic infections in humans and animals
- Neuropeptides and Animal Physiology
- Cytomegalovirus and herpesvirus research
- Immune Cell Function and Interaction
- Pharmacological Effects and Assays
- Aquaculture disease management and microbiota
- Adenosine and Purinergic Signaling
- Polyamine Metabolism and Applications
- Silymarin and Mushroom Poisoning
- Complement system in diseases
- RNA Interference and Gene Delivery
- Acute Ischemic Stroke Management
- Selenium in Biological Systems
- Galectins and Cancer Biology
Nanjing Agricultural University
2015-2024
Bridge University
2020
Creative Commons
2019
University of Science and Technology of China
2019
Anhui Provincial Hospital
2012
Haemonchus contortus is a parasitic gastrointestinal nematode, and its excretory secretory products (HcESPs) interact extensively with the host cells. In this study, we report interaction of proteins from HcESPs at different developmental stages to goat peripheral blood mononuclear cells (PBMCs) in vivo using liquid chromatography-tandem mass spectrometry. A total 407 that interacted PBMCs time points were identified H. protein database SEQUEST searches. The L4 L5 represented higher...
Haemonchosis is a disease of the small ruminant caused by nematode parasite Haemonchus contortus, and it most important alarming challenges to ruminant's production. The infection H. contortus could cause high economic losses worldwide. blood feeding which penetrates into abomasal mucosa feed host causing anemia decreased total plasma protein. Modulation suppression immune response parasites have been reported extensively, cysteine protease inhibitor (cystatin) identified as one major...
Arginine kinase (AK), an important member of phosphagen family has been extensively studied in various vertebrates and invertebrates. Immunologically, AKs are constituents different body parts, involved biological cellular functions, considered as immune-modulator effector for pro-inflammatory cytokines. However, immunoregulatory changes host cells triggered by AK protein Haemonchus contortus, a parasitic nematode ruminants, still unknown. The current study was focused on cloning...
In the present study, microneme 5 gene of Eimeria acervulina (E. acervulina) (EaMIC5) was cloned and characterized. Specific primers for rapid amplification cDNA ends (RACE) were designed based on expressed sequence tag (EST, GenBank Accession No. EH386430.1) to amplify 3′- 5′-ends EaMIC5. The full length this obtained by overlapping sequences 5′-extremities reverse transcription PCR. Sequence analysis revealed that open reading frame (ORF) EaMIC5 336 bp encoded a protein 111 amino acids...
In the present study, immune protective effects of recombinant microneme protein 7 Eimeria maxima (rEmMIC7) and a DNA vaccine encoding this antigen (pVAX1-EmMIC7) on experimental challenge were evaluated. Two-week-old chickens randomly divided into five groups. Experimental groups immunized with 100 μg pVAX1-MIC7 or 200 rEmMIC7, while control injected pVAX1 plasmid sterile phosphate buffered saline (PBS). The results showed that anti-EmMIC7 antibody titres in both rEmMIC7 significantly...
Galectins are glycan-binding proteins that widely expressed and distributed in mammalian tissues as well cells of innate adaptive immune responses. CD4+ T-helper differentiate into effector subsets response to cytokines. T helper 9 one the recently described relatively new less studied. In this study, galectin domain containing protein from Haemonchus contortus (Hc-GDC) was cloned, pET32a, immunoblotting performed. Localization recombinant (r)Hc-GDC on outer inner surface H. worm binding...
Haemonchus contortus (H. contortus) has evolved sophisticated evasion mechanisms to ensure their survival, including generating excretion and secretion products (ESPs) regulate the of host cytokines. Interleukin 4 (IL4) is a classic T-helper cell type 2 (Th2)-type cytokine that plays an irreplaceable role against nematode infection. In this study, three proteins, glutathione S-transferase domain containing protein (HcGST), transthyretin (HcTTR) calponin actin-binding (HcCab), were identified...
Introduction Avian coccidiosis, caused by apicomplexan protozoa belonging to the Eimeria genus, is considered one of most important diseases in intensive poultry industry worldwide. Due shortcomings live anticoccidial vaccines and drugs, development novel increasingly urgent. Methods maxima rhomboid-like protein 1 (EmROM1), an invasion-related molecule, was selected as a candidate antigen evaluate its protective efficacy against E. chickens. Firstly, prokaryotic recombinant plasmid...
It has been reported that infection of chicken coccidian could inhibit the production Th1 cytokine IFN-γ, thereby evading clearance by host immune system. The present study aimed to have a further investigation into effects Eimeria maxima IFN-γ inhibitory molecules (EmHPSP-2 and EmHPSP-3) on function peripheral blood mononuclear cells (PBMC) various T cell subsets. First, separated PBMC or sorted subsets were used for incubation with recombinant proteins EmHPSP-2 (rEmHPSP-2) EmHPSP-3...
Chicken coccidiosis has inflicted significant economic losses upon the poultry industry. The primary strategies for preventing and controlling chicken include anticoccidial drugs vaccination. However, these approaches face limitations, such as drug residues resistance associated with drugs, safety concerns related to live vaccines. Consequently, urgent development of innovative vaccines, subunit is imperative. In previous study, we screened 2 candidate antigens: Eimeria maxima...
Abstract Avian coccidian parasites exhibit a high degree of site specificity in different Eimeria species. Although the underlying mechanism is unclear, an increasing body evidence suggests that due to interaction between microneme proteins (MICs) and their receptors on surface target host cells. In this study, binding ability E. tenella MICs (EtMICs) intestinal tissue was observed by immunofluorescence identify key molecule parasite responsible for specificity. Subsequently, we identified...
Eimeria maxima initiates infection by invading the jejunal epithelial cells of chicken. However, proteins involved in invasion remain unknown. The research molecules that participate interactions between E. sporozoites and host target will fill a gap our understanding system this parasitic pathogen. In present study, chicken were isolated cultured vitro. Western blot was employed to analyze soluble bound cells. Co-immunoprecipitation (co-IP) assay used separate Shotgun LC-MS/MS technique for...
Avian coccidiosis brings tremendous economic loss to the poultry industry worldwide. The third generation vaccine, including subunit and DNA vaccines, exhibited promising developmental prospects. In a previous study, we found rhomboid-like protein 3 of Eimeria maxima (EmROM3) was involved in infections by species. However, protective efficacy EmROM3 against ( E. ) remains unknown. this chickens were intramuscularly immunized with recombinant (rEmROM3) or pVAX1-EmROM3 determine EmROM3-induced...
Excretory/secretory antigens (ESAs) produced by Toxoplasma gondii enable this parasite to invade and survive within the host cells through immunomodulation. In study, modulating effects of T. excretory/secretory (TgESAs) on Ana-1 murine macrophage cell line were evaluated. incubated with several concentrations TgESAs, resulting cellular viability, phagocytotic ability, apoptosis induction determined. Pro-inflammatory anti-inflammatory cytokine secretion, nitric oxide production, toll-like...