A5023287010- DNA Repair Mechanisms
- DNA and Nucleic Acid Chemistry
- Bacterial Genetics and Biotechnology
- Carcinogens and Genotoxicity Assessment
- Advanced biosensing and bioanalysis techniques
- RNA Interference and Gene Delivery
- Heavy Metal Exposure and Toxicity
- Radiation Dose and Imaging
- Glycosylation and Glycoproteins Research
- CRISPR and Genetic Engineering
- Radioactive element chemistry and processing
- MicroRNA in disease regulation
- Plant Genetic and Mutation Studies
- Electron Spin Resonance Studies
- Molecular Sensors and Ion Detection
- Integrated Circuits and Semiconductor Failure Analysis
- COVID-19 and healthcare impacts
- Proteoglycans and glycosaminoglycans research
- Food Safety and Hygiene
- Radioactivity and Radon Measurements
- Advanced oxidation water treatment
- Advances in Oncology and Radiotherapy
- Electron and X-Ray Spectroscopy Techniques
- Healthcare cost, quality, practices
- Wnt/β-catenin signaling in development and cancer
Instituto de Radioproteção e Dosimetria
1984-2023
National Nuclear Energy Commission
2000-2023
Nuclear Engineering Institute
2019-2021
Institut Curie
2002
Universidade Federal do Rio de Janeiro
1994-2000
World Health Organization Regional Office for the Americas
1997
Hydrogen peroxide is an important reactive oxygen species (ROS) that arises either during the aerobic respiration process or as a by-product of water radiolysis after exposure to ionizing radiation. The reaction hydrogen with transition metals imposes on cells oxidative stress condition can result in damage cell components such proteins, lipids and principally DNA, leading mutagenesis death. Escherichia coli are able deal these adverse events via DNA repair mechanisms, which enable them...
Thorium, cerium, and lanthanum are metals present in several types of minerals, the most common which is monazite. Cerium elements lanthanides series. an actinide metal, a hazardous element due to its radioactive characteristics. There lack information describing possible chemical interactions among these effects they may have on humans. Toxicological analyses were performed using cell viability, death, DNA damage assays. Chemical evaluated based Loewe additivity model. The results indicate...
ABSTRACT In Escherichia coli , the repair of lethal DNA damage induced by H 2 O requires exonuclease III, xthA gene product. Here, we report that both endonuclease IV (the nfo product) and III can mediate lesions under low-iron conditions. Neither nor mutants was sensitive to in presence iron chelators, while double mutant significantly this treatment, suggesting formed such Sedimentation studies alkaline sucrose gradients also demonstrated mutants, but not mutant, carry out complete strand...
MUC1, the transmembrane glycoprotein Mucin 1, is usually found to be overexpressed in a variety of epithelial cancers playing an important role disease progression. MUC1 isoforms such as MUC1/Y, which lacks entire variable number tandem repeat region, are involved oncogenic processes by enhancing tumour initiation. MUC1/Y therefore considered promising target for identification and treatment cancers; but so far, precise remains elucidated. In this work, we developed identified DNA aptamer...
Background: Proteins overexpressed in malignant tissues form important targets the development of targeted therapeutics, and aptamers comprise an affinity agent for therapy drug delivery. In this study, aberrantly expressed mucin 1 glycoprotein was investigated as a therapeutic target breast cancer model. Materials Methods: order to determine feasibility using aptamer against (aptA) carrier cytotoxic compound 1,10-phenanthroline MCF-7 cells, potential radiosensitizer, studied experiments...
The objective of the present study was to apply a highly sensitive botanical test mutagenicity (the Tradescantia stamen-hair mutation bioassay) assess in situ biological responses induced by naturally occurring radiation Poços de Caldas Plateau. mutagenesis evaluated environments presenting gamma exposure rates ranging from 1.5 muR.min-1 up 100.0 muR.min-1. results consistently showed only borderline increases frequencies plants exposed areas with high background, as compared non-exposed...
Pretreatment of Escherichia coli cultures with the iron chelator 2,2'-dipyridyl (1 mM) protects against lethal effects low concentrations hydrogen peroxide (<15 mM). However, at H(2)O(2) equal to or greater than 15 mM, dipyridyl pretreatment increases lethality and mutagenesis, which is attributed formation different types DNA lesions. We show here that prior challenge high (>or=15 induced mainly G:C-->A:T transitions (more 100X mM more 250X 20 over spontaneous mutagenesis rate) in E. coli....
Pretreatment of Escherichia coli cultures with the iron chelator 2,2’-dipyridyl (1 mM) protects against lethal effects low concentrations hydrogen peroxide (<15 mM). However, at H2O2 equal to or greater than 15 mM, dipyridyl pretreatment increases lethality and mutagenesis, which is attributed formation different types DNA lesions. We show here that prior challenge high (≥15 induced mainly G:C→A:T transitions (more 100X mM more 250X 20 over spontaneous mutagenesis rate) in E. coli. In...
Cholera returned to South America in January 1991, after almost a century of absence. The hygienic status the countries affected, aggravated by economic and political difficulties, allowed disease spread rapidly. In Peru, fishery products were incriminated from outset, although without conclusive evidence. However, epidemiological laboratory findings other have confirmed transmission these products. authors discuss effects recent cholera epidemic on trade consumption America. actual risk...