A5102826658- Cardiac Fibrosis and Remodeling
- Pluripotent Stem Cells Research
- Tissue Engineering and Regenerative Medicine
- Respiratory Support and Mechanisms
- Congenital heart defects research
- Neonatal Respiratory Health Research
- Coronary Artery Anomalies
- Cardiovascular Function and Risk Factors
- Signaling Pathways in Disease
- Heart Failure Treatment and Management
- Airway Management and Intubation Techniques
- Angiogenesis and VEGF in Cancer
- CRISPR and Genetic Engineering
- Hemodynamic Monitoring and Therapy
- Medical Imaging and Pathology Studies
- Blood Coagulation and Thrombosis Mechanisms
- Pulmonary Hypertension Research and Treatments
- Viral Infections and Immunology Research
- Infective Endocarditis Diagnosis and Management
- Cancer, Hypoxia, and Metabolism
- Renal and Vascular Pathologies
- Occupational exposure and asthma
- Acute Ischemic Stroke Management
- Cardiac, Anesthesia and Surgical Outcomes
- Fibroblast Growth Factor Research
University of Tsukuba
2015-2024
National Hospital Organization Mito Medical Center
2019-2022
Sakai Municipal Hospital
2019
International University of Health and Welfare
2018
Taiho Pharmaceutical (Japan)
1996
Massachusetts General Hospital
1988-1990
Platelet-derived endothelial cell growth factor (PD-ECGF) is known to promote the development of new blood vessels, which are fundamental tumor and metastasis. We previously found that thymidine phosphorylase (dThdPase) PD-ECGF same protein.We retrospectively examined expression dThdPase in primary colorectal carcinomas, its association with angiogenesis clinicopathologic findings, prognostic value.Tissues were obtained from tumors 163 patients whose carcinomas completely removed by surgery....
Because adult cardiomyocytes have little regenerative capacity, resident cardiac fibroblasts (CFs) synthesize extracellular matrix after myocardial infarction (MI) to form fibrosis, leading dysfunction and heart failure. Therapies that can regenerate the myocardium reverse fibrosis in chronic MI are lacking. The overexpression of transcription factors, including Mef2c/Gata4/Tbx5/Hand2 (MGTH), directly reprogram CFs into induced (iCMs) improve function under acute MI. However, ability vivo...
Direct cardiac reprogramming holds great potential for regenerative medicine. However, it remains inefficient, and induced cardiomyocytes (iCMs) generated in vitro are less mature than those vivo, suggesting that undefined extrinsic factors may regulate reprogramming. Previous studies mainly used hard polystyrene dishes, yet the effect of substrate rigidity on unclear. Thus, we developed a Matrigel-based hydrogel culture system to determine roles matrix stiffness mechanotransduction We found...
Cardiac transcription factors (TFs) directly reprogram fibroblasts into induced cardiomyocytes (iCMs), where MEF2C acts as a pioneer factor with GATA4 and TBX5 (GT). However, the generation of functional mature iCMs is inefficient, molecular mechanisms underlying this process remain largely unknown. Here, we found that overexpression transcriptionally activated via fusion powerful MYOD transactivation domain combined GT increased beating by 30-fold. Activated generated were...
The mechanisms underlying pulmonary hypertension (PH) remain largely unknown; further, why advanced vascular remodeling preferentially occurs in arterioles is yet to be answered. VEGF (vascular endothelial growth factor) regulates angiogenesis through Flk1 (fetal liver kinase 1) and Flt1 (fms-like tyrosine on cells (ECs), which may related PH pathogenesis. However, spatiotemporal expression patterns of the system role development poorly understood.We analyzed multiple reporter mice,...
To investigate whether physicians' intrapersonal empathy increased after a communication skills training (CST) workshop.Participants were oncologists from across Japan with three or more years of clinical experience in oncology. They recruited through the Internet and via direct contact by workshop organizers. Participants attended 1 132 two-day CST workshops, held between November 2007 March 2011. Prior to (baseline/T1), participants completed survey demographic questions, Jefferson Scale...
The regional effects of tidal volume (VT), respiratory frequency, and expiratory-to-inspiratory time ratio (TE/TI) during high-frequency ventilation (HFV) were studied in anesthetized paralyzed dogs. Regional per unit lung (spVr) was assessed with a positron camera the washout tracer isotope 13NN from lungs 12 supine From data, functional images mean residence (MRT) produced spVr estimated as inverse MRT. We found that at constant VT X f product (where represents frequency), increasing...
BACKGROUND: Heart failure with preserved ejection fraction (HFpEF) is a major health concern. Pathological stimuli and interactions between cardiac fibroblasts (CFs) other cell types may lead to fibrosis diastolic dysfunction, which are hallmarks of HFpEF. Interstitial perivascular correlates poor prognosis in HFpEF; however, mechanisms remain poorly elucidated, targeted therapies lacking. Cardiac reprogramming promising therapeutic approach for myocardial infarction that facilitates...
The effects of body position and respiratory frequency (f) on regional gas transport during eucapnic conventional ventilation (CV) high-frequency (HFV) were assessed from the washout nitrogen 13 (13NN) using positron-emission tomography. In one protocol, six dogs ventilated with CV or HFV at f = 6 Hz tidal volume (VT) selected supine for eucapnia. A coronal cross section lung base was studied in supine, prone, right left lateral decubitus positions. a second prone: apical basal sections 3 9...
The effects of changing tidal volume (VT) and frequency (f) on the distribution ventilation during high-frequency (HFV) were assessed from washout nitrogen-13 by positron emission tomography. Six dogs, anesthetized paralyzed, studied in supine position conventional (CV) HFV at f 3, 6, 9 Hz. In CV 6 Hz, VT was selected to achieve eucapnic arterial partial pressure CO2 (37 +/- 3 Torr). At proportionally changed so that product remained constant equal Mean residence time (MRT) calculated for...
It has been suggested that the increase in inspiratory flow rate caused by a decrease inspiratory-to-expiratory time ratio (I:E) at constant tidal volume (VT) could efficiency of ventilation high-frequency (HFV). To test this hypothesis, we studied effect changing I:E from 1:1 to 1:4 on steady-state alveolar (VA) given VT and frequency (f) mean lung (VL). In nine anesthetized, paralyzed, supine dogs, HFV was performed 3, 6, 9 Hz with ventilator delivered expiratory rates. Mean airway...
The prognosis of tetralogy Fallot (TOF) treated only with Blalock-Taussig shunt (BTS) operation is unclear. A woman TOF underwent classic BTS at 10 years age. Despite no medication, she delivered two children and worked without apparent heart failure. At 72 age, complained dyspnea on exertion leg edema. cardiac angiogram revealed a well-patent severely stenotic right ventricular outflow tract. Right catheterization showed adequately maintained pulmonary blood flow slight arterial...
Endovascular repair is often difficult in the case of a huge abdominal aortic aneurysm for anatomic reasons. Here, we describe open infrarenal aneurysm. Open was performed through laparotomy with Cattell-Braasch maneuver, technique right-sided medial visceral rotation. Laparotomy maneuver simple and effective extending into right common iliac artery, which proximal clamping because tortuous neck just below hepatic region.
In 10 anesthetized, paralyzed, supine dogs, arterial blood gases and CO2 production (VCO2) were measured after 10-min runs of high-frequency ventilation (HFV) at three levels mean airway pressure (Paw) (0, 5, cmH2O). HFV was delivered frequencies (f) 3, 6, 9 Hz with a ventilator that generated known tidal volumes (VT) independent respiratory system impedance. At each f, VT adjusted Paw 0 cmH2O to obtain eucapnia. As increased 5 cmH2O, PCO2 (PaCO2) PO2 (PaO2) decreased monotonically...
Background: Mitochondrial dysfunction in the heart is associated with development of failure (HF). However, clinical consequences mitochondrial structural abnormalities patients HF remain unexplored.