A5058857190- Microbial Community Ecology and Physiology
- Marine and coastal ecosystems
- Microbial bioremediation and biosurfactants
- Genomics and Phylogenetic Studies
- Vibrio bacteria research studies
- Marine Biology and Ecology Research
- Protist diversity and phylogeny
- Oil Spill Detection and Mitigation
- Plant Virus Research Studies
- Metalloenzymes and iron-sulfur proteins
- Photosynthetic Processes and Mechanisms
- Marine and fisheries research
- Bacteriophages and microbial interactions
- Plant and Fungal Interactions Research
- Circular RNAs in diseases
- Coastal wetland ecosystem dynamics
- Vector-Borne Animal Diseases
- Microbial Fuel Cells and Bioremediation
- Electrocatalysts for Energy Conversion
- Isotope Analysis in Ecology
- Protein Structure and Dynamics
- Water Treatment and Disinfection
- Environmental DNA in Biodiversity Studies
- Pharmaceutical and Antibiotic Environmental Impacts
- Algal biology and biofuel production
University of West Florida
2019-2024
University of Delaware
2005-2016
Elcriton (United States)
2011-2012
Biotechnology Institute
2009
College of Marin
2005-2008
University of Maryland, Baltimore County
2001
Summary To determine whether metagenomic libraries sample adequately the dominant bacteria in aquatic environments, we examined phylogenetic make‐up of a large insert library constructed with bacterial DNA from Delaware River, polymerase chain reaction (PCR) 16S rRNA genes, and community structure determined by fluorescence situ hybridization (FISH). The composition FISH differed for major groups river, which included Actinobacteria , beta‐ proteobacteria Cytophaga ‐like bacteria. Beta‐ were...
Summary Proteorhodopsin (PR) is a light‐driven proton pump that has been found in variety of marine bacteria, including Pelagibacter ubique , member the ubiquitous SAR11 clade. The goals this study were to explore diversity PR genes and estimate their abundance North Atlantic Ocean using quantitative polymerase chain reaction (QPCR). We western portion Sargasso Sea could be grouped into 27 clusters, but five clades had most sequences. Sets specific QPCR primers designed examine following...
Aerobic anoxygenic phototrophic (AAP) bacteria are photoheterotrophs that, if abundant, may be biogeochemically important in the oceans. We used epifluorescence microscopy and quantitative PCR (qPCR) to examine abundance of these by enumerating cells with bacteriochlorophyll a (bChl a) light-reaction center gene pufM, respectively. In surface waters Delaware estuary, AAP were comprising up 34% prokaryotes, although percentage varied greatly location season. On average, made 12% community as...
The diversity of aerobic anoxygenic phototrophic (AAP) bacteria has been examined in marine habitats, but the types AAP estuarine waters and distribution ecotypes any environment are not well known. goal this study was to determine Delaware estuary examine select using quantitative PCR (qPCR) assays for pufM gene, which encodes a protein light reaction center bacteria. In libraries from River, genes similar those Beta- (Rhodoferax-like) or Gammaproteobacteria comprised at least 50% clones,...
Investigations of the relationships between crude oil and bacterioplankton have provided clues into how constituents are degraded, anthropogenic spills affect system nutrient cycling food webs. The Deepwater Horizon spill in 2010 re-invigorated investigation marine bacteria respond to spills. While much historical research initial efforts focused on biodegradation oil, it became quickly apparent that bacterial community was affected negatively by oil. Environmental conditions often altered...
Photosynthesis genes and operons of aerobic anoxygenic photosynthetic (AAP) bacteria have been examined in a variety marine habitats, but genomic information about freshwater AAP is lacking. The goal this study was to examine photosynthesis the Delaware River. In fosmid library, we found two clones bearing gene clusters with unique content organization. Both contained 37 open reading frames, most those encoding known bacterial proteins. one were closely related Rhodobacter genus. other clone...
3-Nitro-1,2,4-triazol-5-one (NTO) is one of the main ingredients many insensitive munitions, which are being used as replacements for conventional explosives. As its use becomes widespread, more research needed to assess environmental fate. Previous studies have shown that NTO biologically reduced 3-amino-1,2,4-triazol-5-one (ATO). However, final degradation products ATO still unknown. We studied aerobic by enrichment cultures derived from soil. After multiple transfers, was monitored in...
The 2010 Deepwater Horizon (DwH) Oil spill released an enormous volume of oil into the Gulf Mexico (GoM), prompting widespread use chemical dispersants like Corexit ® EC9500A. ecological consequences this treatment, especially when combined with natural factors such as sunlight, remain unexplored in context marine bacterial communities’ dynamics. To address knowledge gap, our study employed a unique metaproteomic approach, investigating effects crude Macondo surrogate oil, and on GoM...
The diversity and abundance of glycosyl hydrolase family 5 (GH5) were studied in the North Atlantic Ocean. This was chosen because large number available sequences from cultured bacteria, variety substrates it targets, high similar Sargasso Sea environmental genome database. Three clone libraries a GH5 subcluster constructed Mid-Atlantic Bight eastern western two Ocean did not differ each other but both significantly less diverse than library. genes estimated by quantitative PCR positively...
Viral production estimates show that virioplankton communities turn over rapidly in aquatic ecosystems. Thus, it is likely the genetic identity of viral populations comprising also change temporal and spatial scales, reflecting shifts viral-host interactions. However, there are few approaches can provide data on genotypic at low cost with sample throughput necessary to assess dynamic changes virioplankton. This study examined two these approaches-T4-like major capsid protein (g23) gene...
The chemical synthesis intermediate 3,4-dichloronitrobenzene (3,4-DCNB) is an environmental pollutant. Diaphorobacter sp. strain JS3050 utilizes 3,4-DCNB as a sole source of carbon, nitrogen and energy. However, the molecular determinants its catabolism are poorly understood. Here, complete genome was sequenced key genes were expressed heterologously to establish details degradation pathway. A chromosome-encoded three-component nitroarene dioxygenase (DcnAaAbAcAd) converted...
Algae generate hydrogen from sunlight and water utilizing high-energy electrons generated during photosynthesis. The amount of produced in heterologous expression the wild-type hydrogenase is currently insufficient for industrial applications. One approach to improve yields through directed evolution DNA native hydrogenase. Here, we created 113 chimeric algal gene variants derived combining segments three parent hydrogenases, two Chlamydomonas reinhardtii (CrHydA1 CrHydA2) one Scenedesmus...
An invasive population of the Asian shore crab Hemigrapsus sanguineus was discovered in 1988 near mouth Delaware Bay, and populations now occur from North Carolina to Maine. The H. competes with indigenous species has displaced resident crabs throughout its range. However, there have been few studies that document changes after become established.We compare sympatric a native mud (Panopeus herbstii) were monitored initially 2001 again 2011 2012. historical study conducted rocky habitat Cape...
Vibriosis is the general term for human illnesses caused by infection of pathogenic Vibrio species. vulnificus ( Vv ) and parahaemolyticus Vp are two problematic waterborne pathogens that have yet to be enumerated in northwest Florida coastal Gulf Mexico estuaries. In this regionally novel study, we surveyed 43 locations subtropical estuarine systems, Perdido Bay Pensacola Bay, over seven dates winter 2020. Sampling included three substrate types: surface waters, sediments, invertebrate...
The increased potential for contamination of seawater by crude oils requires studies bacterial biodegradation potential, but little is known the differential negative impacts on growth. No two wells generate chemically identical oils; and importantly, solar exposure oil may differentially affect response. Elucidating role that sunlight plays toxicity spilled imperative to understanding how spills might microbes in tropical subtropical waters Florida. This study examined light six different...