A5062423101- Biosensors and Analytical Detection
- Advanced biosensing and bioanalysis techniques
- Electrowetting and Microfluidic Technologies
- Innovative Microfluidic and Catalytic Techniques Innovation
- SARS-CoV-2 detection and testing
- Advanced Biosensing Techniques and Applications
- Microfluidic and Capillary Electrophoresis Applications
- 3D Printing in Biomedical Research
- Microfluidic and Bio-sensing Technologies
- Bacteriophages and microbial interactions
- Cancer Cells and Metastasis
- Cancer Research and Treatments
- Molecular Biology Techniques and Applications
- Mycobacterium research and diagnosis
- Advanced Chemical Sensor Technologies
- Tuberculosis Research and Epidemiology
- Nanoparticle-Based Drug Delivery
- Nanoplatforms for cancer theranostics
- Green IT and Sustainability
- Microbial Community Ecology and Physiology
- Graphene and Nanomaterials Applications
- QR Code Applications and Technologies
- Microbial infections and disease research
- Aquaculture disease management and microbiota
- Mobile Crowdsensing and Crowdsourcing
Indian Institute of Science Bangalore
2018-2025
Indra (Spain)
2021
Seattle University
2015-2016
University of Washington
2012-2016
University of Massachusetts Amherst
2010-2013
Weatherford College
2013
Amherst College
2012
In-Q-Tel
2011
This diagnostic system prototype is the first demonstration of a fully integrated, simple-to-use nucleic acid amplification test device.
We demonstrate a novel method for controlling fluid flow in paper-based devices. The delays progress through porous channel by diverting into an absorbent pad-based shunt placed contact with the channel. Parameters to control delay include length and thickness of shunt. Using this method, reproducible ranging from 3 20 min were achieved. A simple electrical circuit model was presented used predict system. Results showed good agreement experimental observations. Finally, shunts sequential...
A versatile, powerless, valving and automation toolkit for paper microfluidic devices that uses absorbent expanding elements as actuators is presented.
Journal Article Motility is critical for effective distribution and accumulation of bacteria in tumor tissue Get access Bhushan J. Toley, Toley 159 Goessman Laboratory, Department Chemical Engineering, University Massachusetts, Amherst, 686 North Pleasant Street, MA 01003-9303, USA. Fax: +1 (413) 545-1647; Tel: 577-0132 Search other works by this author on: Oxford Academic Google Scholar Neil S. Forbes E-mail: forbes@ecs.umass.edu Integrative Biology, Volume 4, Issue 2, February 2012, Pages...
We describe a method for fabricating paper-based microfluidic devices using commercially available CO2 laser system. The is versatile and allows controlled through-cutting ablative etching of nitrocellulose substrates. In addition, the system can cut variety components that are useful in fabrication devices, including cellulose wicking pads, glass fiber source pads Mylar-based substrates device housing.
A new rapid and sensitive method of isothermal DNA amplification a simple kinetic model this reaction network.
Paper-based microfluidic devices are rapidly becoming popular as a platform for developing point-of-care medical diagnostic tests. However, the design of these largely relies on trial and error, owing to lack proper understanding fluid flow through porous membranes. Any material having pores multiple sizes contains partially saturated regions, i.e., regions where less than 100% filled with fluid. The capillary pressure permeability change function extent saturation. Although methods measure...
The global need for disease detection and control has increased effort to engineer point-of-care (POC) tests that are simple, robust, affordable, non-instrumented. In many POC tests, sample collection involves swabbing the site (e.g., nose, skin), agitating swab in a fluid release sample, transferring device analysis. Poor performance transfer can reduce sensitivity reproducibility.In this study, we compared bacterial efficiency of seven types using manual-agitation methods typical devices....
This study presents "hot-nucleic acid lateral flow assay" (Hot-NALFA), an innovative method for detecting point mutations in nucleic sequences. Hot-NALFA integrates the temperature-sensitive allelic discrimination properties of molecular beacons into a assay format operating at elevated temperatures (~45–55°C). approach enables end-point, visual detection mutations, eliminating requirement real-time fluorescence instrumentation traditionally associated with beacon-based discrimination. The...
Bacterial infections remain a global threat, particularly in low-resource settings, where access to accurate and timely diagnosis is limited. Point-of-care nucleic acid amplification tests have shown great promise addressing this challenge. However, their dependence on complex traditional sample preparation methods remains major To address limitation, we present paper-based device that integrates bacterial cell lysis, DNA purification, concentration using an electrokinetic technique called...
We present a novel method for fabricating microfluidic paper-based analytical devices (µPADs) by laser melting of Parafilm wax to form hydrophobic barriers. This approach involves placing on filter paper and patterning it with CO₂ laser, which locally melts the create embedded flow Crucially, is peeled off post-patterning, preserving both surfaces enabling vertical assays. Optimal parameters were determined ensure consistent barrier formation without damaging paper. The technique enabled...
Journal Article Microfluidic technique to measure intratumoral transport and calculate drug efficacy shows that binding is essential for doxorubicin release hampers Doxil Get access Bhushan J. Toley, Toley Department of Chemical Engineering University Massachusetts, Amherst, 159 Goessmann Laboratory, 686 North Pleasant Street, MA 01003-9303, USA. Fax: +1 413-545-1647; Tel; 413-577-0132 Search other works by this author on: Oxford Academic Google Scholar Zachary G. Tropeano Lovatt, Lovatt...
Abstract We present a prototype for conducting rapid, inexpensive and point-of-care-compatible nucleic acid amplification tests (NAATs) tuberculosis (TB). The fluorescent isothermal paper-and-plastic NAAT (FLIPP-NAAT) uses paper-based loop mediated (LAMP) DNA detection. cost of materials required to build 12-test-zone device is $0.88 the reagents per reaction $0.43. An imaging platform enables filter-free fluorescence detection amplified using cell-phone camera. FLIPP-NAAT can be operated by...
In recent years, microfluidic paper analytical devices (μPADs) have been extensively utilized to conduct multiplex colorimetric assays. Despite their simple and user-friendly operation, the need for patterning with wax or other physical barriers create flow channels makes large-scale manufacturing cumbersome. Moreover, convection of rehydrated reagents in test zones leads nonuniform signals, which quantification difficult. To overcome these challenges, we present a device called barrier-free...
Bacterial therapies have the potential to overcome resistances that cause chemotherapies fail. When using bacteria produce anticancer agents in tumors, triggering gene expression is necessary prevent systemic toxicity. The use of chemical triggers, however, hampered by poor delivery inducing molecules, which reduces number activated bacteria. To solve this problem, we created a cell-communication system enables induce inactive neighbors. We hypothesized introducing cell communication into...
Lateral flow tests (LFTs) are well-suited for rapid point-of-care testing in low resource settings. The wicking action of the paper strip moves sample and reagents through device without a need pumps, but LFTs typically limited to that can be carried out single fluidic step. materials from reconfigured create networks automatically carry multi-step operations, while retaining same easy-to-use format as conventional LFT. Here, we describe basic principles system-level behavior by analogy...
Paper-based microfluidic devices are popular for their ability to automate multistep assays chemical or biological sensing at a low cost, but the design of paper networks has largely relied on experimental trial and error. A few mathematical models flow through have been developed succeeded in explaining behavior. However, reverse engineering problem designing complex guided by appropriate is unsolved. In this article, we demonstrate that two-dimensional network (2DPN) designed sequentially...
Abstract Spatially uniform reconstitution of dried reagents is critical to the function paper microfluidic devices. Advancing fluid fronts in devices drive (convect) and concentrate rehydrated edges, causing steep chemical gradients imperfect mixing. This largely unsolved problem microfluidics exacerbated by increasing device dimensions. In this article, we demonstrate that mixing with a rehydrating may be significantly enhanced stacking layers having different wicking rates. Compared...
Testing the quality of soil is essential for appropriate fertilizer use. However, testing currently conducted in dedicated centralized facilities equipped with expensive analytical instruments, not easily accessible to many farmers lower-income agrarian economies like India. In an attempt address this gap, we present a prototype colorimetric detection and quantification macronutrients – nitrogen (N) phosphorous (P). For this, employ technology previously developed our laboratory called...
The lateral flow immunoassay (LFIA) is widely adopted for point-of-care testing, but its limit of detection (LoD) falls short that laboratory-based immunoassays. Several techniques have been proposed to enhance the LoD LFIAs using visual colorimetric readouts, yet a direct comparison LoDs achieved by these has not performed. In this work, we measure designed malaria protein, PfHRP2, four different signal generation techniques: (i) AuNP(40 nm)-tagged antibodies (base case), (ii) AuNP-based...