A5082122104- Cancer Research and Treatments
- Nanoplatforms for cancer theranostics
- Virus-based gene therapy research
- Bacteriophages and microbial interactions
- 3D Printing in Biomedical Research
- Cancer Cells and Metastasis
- Nanoparticle-Based Drug Delivery
- Microfluidic and Bio-sensing Technologies
- Cancer, Hypoxia, and Metabolism
- Mathematical Biology Tumor Growth
- RNA Interference and Gene Delivery
- Radiation Therapy and Dosimetry
- Advanced Drug Delivery Systems
- Microfluidic and Capillary Electrophoresis Applications
- Gut microbiota and health
- Advanced biosensing and bioanalysis techniques
- Innovative Microfluidic and Catalytic Techniques Innovation
- Probiotics and Fermented Foods
- Pancreatic and Hepatic Oncology Research
- Gene Regulatory Network Analysis
- Biomedical and Engineering Education
- Metabolomics and Mass Spectrometry Studies
- Clostridium difficile and Clostridium perfringens research
- Microbial Metabolic Engineering and Bioproduction
- Microtubule and mitosis dynamics
University of Massachusetts Amherst
2015-2025
Amherst College
2012-2025
University of Strathclyde
2018-2023
In-Q-Tel
2011-2015
Pioneer (United States)
2008-2014
Weatherford College
2013
Boston Children's Hospital
2013
Baystate Medical Center
2008
University of California, Berkeley
2001-2006
Massachusetts General Hospital
2001-2003
We demonstrate here the effective delivery of a dye payload into cells using 2-nm core gold nanoparticles, with release occurring via place exchange glutathione onto particle surface. In vitro experiments drug analogues upon addition glutathione. Cell culture show rapid uptake nanoparticle and payload. The role in process was demonstrated through improved transient increase levels achieved introduction ethyl ester cell.
Gold colloids functionalized with amino acids provide a scaffold for effective DNA binding subsequent condensation. Particles lysine and dendron functionality formed particularly compact complexes provided highly efficient gene delivery without any observed cytotoxicity. Nanoparticles first generation dendrons (NP−LysG1) were ∼28-fold superior to polylysine in reporter expression. These acid-based nanoparticles responsive intracellular glutathione levels, providing tool controlled release...
Abstract Critical cancer pathways often cannot be targeted because of limited efficiency crossing cell membranes. Here we report the development a Salmonella-based intracellular delivery system to address this challenge. We engineer genetic circuits that (1) activate regulator flhDC drive invasion and (2) induce lysis release proteins into tumor cells. Released protein drugs diffuse from Salmonella containing vacuoles cellular cytoplasm where they interact with their therapeutic targets....
Light and life: A photolabile gold nanoparticle has been constructed to serve as a DNA carrier. UV irradiation causes the reversal of surface charge, resulting in effective release reactivation suppressed transcription vitro. This effect was also observed living cells, together with efficient internalization into nucleus. Supporting information for this article is available on WWW under http://www.wiley-vch.de/contents/jc_2002/2006/z600214_s.pdf or from author. Please note: The publisher not...
Abstract The effectiveness of most chemotherapeutics is limited by their inability to penetrate deep into tumor tissue and ineffectiveness against quiescent cells. Motile Salmonella typhimurium, which are specifically attracted compounds produced cancer cells, could overcome this therapeutic barrier. We hypothesized that individual chemoreceptors target S. typhimurium specific microenvironments. To test hypothesis, we used time-lapse fluorescent microscopy cylindroids quantify the...
Abstract Multi‐drug resistance greatly limits the efficacy of conventional blood‐born chemotherapeutics, which have limited ability to penetrate tumor tissue and are ineffective at killing quiescent cells far from vasculature. Nonpathogenic, motile bacteria can overcome both theses limitations. We hypothesize that accumulation S. typhimurium in tumors is controlled by two mechanisms: (1) chemotaxis towards compounds produced cancer (2) preferential growth within tissue. tested this...
An effective cancer therapeutic must selectively target tumours with minimal systemic toxicity. Expression of a cytotoxic protein using Salmonella typhimurium would enable spatial and temporal control delivery because these bacteria preferentially over normal tissue. We engineered non-pathogenic S. to secrete murine TNF-related apoptosis-inducing ligand (TRAIL) under the prokaryotic radiation-inducible RecA promoter. The response promoter radiation was measured fluorometry immunoblotting....
Journal Article Motility is critical for effective distribution and accumulation of bacteria in tumor tissue Get access Bhushan J. Toley, Toley 159 Goessman Laboratory, Department Chemical Engineering, University Massachusetts, Amherst, 686 North Pleasant Street, MA 01003-9303, USA. Fax: +1 (413) 545-1647; Tel: 577-0132 Search other works by this author on: Oxford Academic Google Scholar Neil S. Forbes E-mail: forbes@ecs.umass.edu Integrative Biology, Volume 4, Issue 2, February 2012, Pages...
Significance Nonpathogenic Salmonella localize to tumors and can be engineered secrete anticancer proteins, but tumor-specific expression is essential prevent systemic toxicity. To reduce unwanted side effects in healthy tissue, we integrated with a quorum-sensing (QS) switch that only initiates drug the tightly packed colonies present within tumors. Using an vitro 3D-tumor-on-a-chip device vivo mouse models, show QS specifically protein cancerous tissue while remaining uninduced livers....
The heterogeneity of cellular microenvironments in tumors severely limits the efficacy most cancer therapies. We have designed a microfluidic device that mimics microenvironment gradients present will enable development more effective Tumor cell masses were formed within micron-scale chambers exposed to medium perfusion on one side create linear nutrient gradients. optical accessibility PDMS and glass enables quantitative transmitted fluorescence microscopy all regions masses. Time-lapse was...
Bacterial therapies, designed to manufacture therapeutic proteins directly within tumors, could eliminate cancers that are resistant other therapies. To be effective, a payload protein must secreted, diffuse through tissue, and efficiently kill cancer cells. date, these properties have not been shown for single protein. The gene Staphylococcus aureus α-hemolysin (SAH), pore-forming protein, was cloned into Escherichia coli. These bacteria were injected tumor-bearing mice volume measured over...
The aim of this work was to assess the impact solvent selection on microfluidic production liposomes. To achieve this, liposomes were manufactured using small-scale and bench-scale microfluidics systems three aqueous miscible solvents (methanol, ethanol or isopropanol, alone in combination). Liposomes composed different lipid compositions these characterised investigate influence liposome attributes. Our studies demonstrate that is a key consideration during manufacturing process, not only...
Licht und Leben: Ein photolabiles Goldnanopartikel, das als DNA-Transporter dient, wurde konstruiert. UV-Bestrahlung bewirkt die Umkehrung der Nanopartikel-Oberflächenladung, was in vitro zu einer effektiven Freisetzung von DNA Reaktivierung unterdrückter DNA-Transkription führt. Dieser Effekt auch lebenden Zellen beobachtet, denen zudem effizient vom Zellkern aufgenommen wird.