A5102021324- Cellular Mechanics and Interactions
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Cell Adhesion Molecules Research
- Immunotherapy and Immune Responses
- Cellular transport and secretion
- CAR-T cell therapy research
- Force Microscopy Techniques and Applications
- Microbial Metabolism and Applications
- Healthcare Systems and Practices
- Bacterial biofilms and quorum sensing
- Monoclonal and Polyclonal Antibodies Research
- Cardiomyopathy and Myosin Studies
- Endoplasmic Reticulum Stress and Disease
- Molecular Junctions and Nanostructures
- Autoimmune Bullous Skin Diseases
- Nanowire Synthesis and Applications
- Mitochondrial Function and Pathology
- Lipid Membrane Structure and Behavior
- Toxoplasma gondii Research Studies
- Retinal Development and Disorders
- Reproductive System and Pregnancy
- Inflammatory mediators and NSAID effects
- Viral Infectious Diseases and Gene Expression in Insects
- Herpesvirus Infections and Treatments
Institut de Biologie Intégrative de la Cellule
2020-2024
Institut National de Recherche en Santé Publique
2023-2024
Centre National de la Recherche Scientifique
1995-2024
CEA Paris-Saclay
2009-2024
Commissariat à l'Énergie Atomique et aux Énergies Alternatives
2009-2024
Université Paris-Saclay
2016-2024
École Polytechnique
2016
Université Paris-Sud
2016
Laboratoire d'Enzymologie et Biochimie Structurales
2013
Institut de Biologie et Technologies
2010-2011
In the endoplasmic reticulum (ER), newly synthesized subunits of T-cell antigen receptor (TCR), membrane-bound immunoglobulin (mIg), and major histocompatibility complex (MHC) class I antigens must fold correctly assemble completely into multimeric protein complexes prior to transport cell surface. Although folding assembly may occur spontaneously, concept that molecular chaperones facilitate these events is emerging. Here, an intracellular 90-kDa apparent mass, denoted IP90, was shown be ER...
A cDNA clone encoding the human endoplasmic reticulum (ER) resident protein IP90 was isolated and sequenced. It predicts a transmembrane with large ER luminal region showing sequence similarity to calreticulin short cytoplasmic domain containing COOH-terminal RKPRRE that may be relevant its retention in ER. is 95% homologous canine membrane phosphorprotein called pp90 or calnexin (Wada, I., Rindress, D., Cameron, P. H., Ou, W.-J., Doherty, J. J., II, Louvard, Bell, A. W., Dignard, Thomas, D....
The intracellular bacterial pathogen Listeria monocytogenes moves inside the host‐cell cytoplasm propelled by continuous actin assembly at one pole of bacterium. This process requires expression surface protein ActA. Recently, in order to identify regions ActA which are required for assembly, we and others have expressed different domains transfection eukaryotic cells. As this type approach cannot address role actin‐driven propulsion, now generated several L. strains expressing analysed...
Abstract The nucleotidyl cyclase toxin ExoY is one of the virulence factors injected by Pseudomonas aeruginosa type III secretion system into host cells. Inside cells, it activated an unknown eukaryotic cofactor to synthesize various cyclic nucleotide monophosphates. ExoY-like adenylate cyclases are also found in Multifunctional-Autoprocessing Repeats-in-ToXin (MARTX) toxins produced Gram-negative pathogens. Here we demonstrate that filamentous actin (F-actin) hitherto ExoY. Association with...
The evolutionarily conserved Arp2/3 complex plays a central role in nucleating the branched actin filament arrays that drive cell migration, endocytosis, and other processes. To better understand regulation, we used single-particle electron microscopy to compare structures of bound three different inhibitory ligands: glia maturation factor (GMF), Coronin, Arpin. Although inhibitors have distinct binding sites on complex, they each induced an "open" nucleation-inactive conformation. Coronin...
Dendritic actin networks grow in an autocatalytic manner starting from the uncapped minifilament of dynactin.
Actin assembly is involved in cell motility and intracellular movement of Listeria monocytogenes. Induction actin tails mediated by the surface protein ActA. The N-terminal domain ActA sufficient for this function. Cell components known to play a role actin-based are VASP (vasodilatator-stimulated phosphoprotein), multiprotein Arp2/3 complex cofilin. interacts with central Proteins interacting have not been identified. To identify novel host ActA-induced tails, we used bovine brain extracts...
Small GTPase Rabs are required for membrane protein sorting/delivery to precise domains. Rab13 regulates epithelial tight junction assembly and polarized transport. Here we report that Molecule Interacting with CasL (MICAL)-like1 (MICAL-L1) interacts GTP-Rab13 shares a similar domain organization MICAL. MICAL-L1 has calponin homology (CH), LIM, proline rich coiled-coil It is associated late endosomes. Time-lapse video microscopy shows green fluorescent protein-Rab7 mcherry-MICAL-L1 present...
In the present study, we developed human non-MHC-restricted CTL clones from peripheral blood mononuclear cells activated in vitro with recombinant IL 2 and subsequently expended PHA. The CD3/Ti+ were selected for their ability to exhibit reactivity by killing various tumor cell lines culture, including line K562 which does not express MHC antigens. We report that, at least some of NK-like T clones, it is possible establish an allo-CTL activity, that CD3-associated surface antigen recognition...
The cell surface expression of alpha:beta heterodimer was studied using WT31 monoclonal antibody, in peripheral blood lymphocytes (PBL) from a patient who developed prolonged immunodeficiency after allogeneic bone marrow transplantation. This patient, grafted for chronic myelogenous leukemia, received T depleted her HLA, A, B, D matched sibling. late occurrence opportunistic infection, led us to analyze the phenotype PBL. 70% PBL were CD3+ and 29% WT31+, indicating that majority did not...
The N-terminal region of the Listeria monocytogenes ActA protein, in conjunction with host cell factors, is sufficient for actin polymerization at bacterial surface. Previous data suggested that could protect barbed ends from capping proteins. We tested this hypothesis by experiments presence fragment and protein. does not In contrast, polypeptide prevents PIP2 inhibiting activity Gel filtration tryptophan fluorescence showed purified binds to PIP, defining phosphoinositides as novels...
Toxofilin is a 27 kDa protein isolated from the human protozoan parasite Toxoplasma gondii, which causes toxoplasmosis. binds to G-actin, and in vitro studies have shown that it controls elongation of actin filaments by sequestering monomers. affinity for G-actin controlled phosphorylation status its Ser53, depends on activities casein kinase II type 2C serine/threonine phosphatase (PP2C). To get insights into functional properties toxofilin, we undertook structure–function analysis using...
A small percentage of circulating CD3+ cells express a heterodimeric gamma delta receptor. Most these do not the surface marker CD4 and only fraction them bear CD8 molecules. The specificity function TCR-gamma are unclear. We obtained murine mAb produced against an IL-2-dependent human T cell clone defining novel molecule sTA which is expressed on resting peripheral blood but strongly delta-bearing clones. Like receptors for growth factors such as IL-2, Ag present clones lines according...
Understanding the mechanisms of assembly and disassembly macromolecular structures in cells relies on solving biomolecular interacFons. However, those interacFons o_en remain unclear because tools to track molecular dynamics are not sufficiently resolved Fme or space. In this study, we present a straighaorward method for resolving inter- intra- adhesive machinery, using Quantum Dot based Förster Resonance Energy Transfer (FRET) nanosensors. IniFally, measured interacFon between Talin,...
Understanding the mechanisms of assembly and disassembly macromolecular structures in cells relies on solving biomolecular interactions. However, those interactions often remain unclear because tools to track molecular dynamics are not sufficiently resolved time or space. In this study, we present a straightforward method for resolving inter- intra- cell adhesive machinery, using quantum dot (QD) based Förster resonance energy transfer (FRET) nanosensors. Using mechanosensitive protein...
Abstract Understanding the mechanisms of assembly and disassembly macromolecular structures in cells relies on solving biomolecular interactions. However, those interactions often remain unclear because tools to track molecular dynamics are not sufficiently resolved time or space. In this study, we present a straightforward method for resolving inter‐ intra‐molecular cell adhesive machinery, using quantum dot (QD) based Förster resonance energy transfer (FRET) nanosensors. Using...
HLA‐A, B, DR phenotype frequencies were studied in 69 homosexual patients with persistent generalized lymphadenopathy (PGL), 8 LAV/HTLV III related thrombocytopenic purpura and 21 controls. Antigen DR5 was significantly increased PGL patient groups as compared to Our findings suggest that genetic factors associated HLA‐DR5 antigen may predispose individuals infected by the virus occurrence of and/or thrombocytopenia.