A5084502683- Genetic and phenotypic traits in livestock
- Meat and Animal Product Quality
- Animal Nutrition and Health
- Agriculture and Biological Studies
- Genetic Mapping and Diversity in Plants and Animals
- Cancer-related molecular mechanisms research
- Animal Genetics and Reproduction
- Food Industry and Aquatic Biology
- Nutrition, Genetics, and Disease
- Cytokine Signaling Pathways and Interactions
- Muscle Physiology and Disorders
- Neurological Disorders and Treatments
- Virus-based gene therapy research
- Cancer Immunotherapy and Biomarkers
- Molecular Biology Techniques and Applications
- Digestive system and related health
- Immune Response and Inflammation
- Lipid metabolism and biosynthesis
- Renal and related cancers
- Birth, Development, and Health
- Blood Pressure and Hypertension Studies
- Immunotherapy and Immune Responses
- Heat shock proteins research
- Immune Cell Function and Interaction
- Calpain Protease Function and Regulation
North-Caucasus Federal University
2022-2024
Federal Agrarian Scientific Center of the North-East named after NV Rudnitsky
2022-2023
Stavropol State Medical University
2021
Stavropol State Agrarian University
2014-2018
Cattle breeding, including sheep farming, is an important sector of agriculture. Increasing productivity and improving meat quality are considered today as the priorities in industry. Significant advances have been achieved breeding through use genetics. The commonplace all selection programs using manufacturers selected on basis offspring, relatives or ancestors. At same time, achievements molecular genetics can lead to a new methodological level. problem finding reliable communication...
In a group of Karachaevsky rams, genome-wide associations analysis single nucleotide polymorphisms (SNPs) with live parameters meat production was performed. We used for genotyping the Ovine Infinium HD BeadChip 600 K, which consists points to detection 606,000 polymorphisms. A total 12 SNPs found be significantly associated quality corpus and legs ultrasonic traits. this case, 11 candidate genes were described, polymorphic variants can change in sheep body parameters. exons, introns, other...
marker assisted selection methods of sheep require the identification genes that positively and negatively affect meat quality. Genes with high expression levels could have greatest impact on growth structure muscle fibers. Objective: this study evaluated in loin Dzhalginsky Merino sheep. Methods: reverse transcription-quantitative real-time PCR (RT-qPCR) was used to investigate 48 bred Russia. Results: GAPDH, PYGM, CAST, ATP5G1, CAPN3, SOD1, VEGFA, CALM2, YWHAZ, ASIP, MYOD1, CAPN1,...
One of the new promising candidate genes defining productive qualities sheep is MEF2B. Protein from MEF2 group encoded by it affects production myostatin and expression responsible for growth skeletal muscle fibers. Thus, knowledge MEF2B gene structure important genomic selection. We have studied at Severokavkazskaya breed bred in Russia. To detect alleles we use NimbleGen sequencing technology Roche (USA). As a result, was revealed 14 single nucleotide polymorphisms (SNP) given breed. The...
In a group of Jalgin merino rams with no significant influence on the dispersion phenotypes known productivity genes (MSTN, MEF2B, FABP4, etc.), genome-wide search for associations individual polymorphisms intravital indicators meat was performed. Using Ovine Infinium HD BeadChip 600K, 606,000 genome loci were evaluated. Twenty-three substitutions found to be significantly associated external measurements body and ultrasonic parameters. This made it possible describe 14 candidate genes,...
An understanding of what effects particular genes can have on body parameters in productive animals is particularly significant for the process marker-assisted selection. The gene transcriptional factor CCAAT/enhancer-binding protein delta (CEBPD gene) involved growth and known to be a promising candidate use as genomic marker. structure CEBPD locus was determined using NimbleGen sequencing technology (Roche, USA). effect polymorphisms, which were identified aforementioned technology,...
Abstract. For breeding work in order to increase productivity indicators sheep, it is necessary use the most informative exterior parameters. This dictates need develop new approaches assessing phenotypic Purpose. Development of parameters for live assessment meat sheep Dzhalginskiy merino breed and study their significance using method principal components. Scientific novelty. We have proposed several lifetime studied possibility breed. Methods. The was life determination size individual...
The aim of this study was to investigate the influence MyoD1 gene polymorphisms on some body parameters in sheep.MyoD1 were detected using NimbleGen sequencing technology (Roche, USA).The effect identified investigated Stavropol sheep breed rams (n=30).In coding and regulatory parts 26, single nucleotide (SNP) detected.These substitutions: c.-2112C>G, c.-1806A>G, c.
Genomic breeding methods of sheep require the identification new genes that affect quality meat.Using technology reverse transcription-quantitative real-time PCR (RT-qPCR) we investigated expression 48 in loin muscle.Samples were collected from 19 rams (10 with high and 9 low live weight) Manych Merino breed, bred Russia.The GAPDH, PYGM, CAPN3, CAST, ATP5G1, CALM2, SOD1, ASIP VEGFA showed highest expression.The group a medium level included ATOX1, CAPN1, GHR, IGF2, SS18L2, YWHAZ, MYOD1, FOS,...
To improve the breed characters of farm animals, there is need for breeding based on reliable information about particular which key to high-quality work. In this regard, a search new methods identification animals. The article presents an analysis achievements modern genetics, are used obtain more accurate when determining ani-mals. A brief description species and animals molecular DNA markers given. Methods study polymorphism mitochondrial microsatellites analyzed. Spe-cial attention in...
To determine the loci associated with pedigree traits, a genome-wide search was conducted for associations of 275 heads Russian sheep breeds genotyped using 600 thousand single nucleotide polymorphisms (SNPs). The studies were according to “case-control” type, where “case” group is represented by animals North Caucasian meat and wool breed, “control” included other (Karachay, Romanovskaya, Dzhalginsky merino merino). In this study, over 100 SNPs identified highly reliable differences in...
Identification of candidate genes and genetic markers associated with meat productivity in sheep using genome-wide association searches (genome-wide studies, GWAS) is a promising area research. We conducted search for associations single nucleotide polymorphisms (SNPs) thicker fat the lumbar region Russian beef Merino sheep. The object study was rams aged 12 months (n = 50). Fat density achieved at 1st–2nd spine level portable ultrasound scanner. Animal genotyping done Ovine Infinium HD...
For the mass use of genotyping by sequencing in sheep Russian Meat Merino breed, it is necessary to determine loci genome with a sufficient frequency occurrence population. To identify them, was carried out using Ovine Infinium HD BeadChip 600K. As result polymorphism evaluation 606,000 loci, 555 SNPs were selected both homozygous variants range 0.2850-0.3149. After excluding substitutions located closer than 1cM, list 387 polymorphisms obtained. The on all 26 autosomes. greatest number 1,...
The domestic Kalmyk cattle breed is considered unique and differs from all meat breeds bred in Russia. Animals of this are endowed with natural endurance independence climatic conditions, they characterized by high adaptive ability, which makes it possible to them both purity various schemes crossing other many regions our country. In addition, beef animals has quite quality taste indicators. Given the increasing need for high-quality beef, research aimed at productivity an urgent task...
Genome-wide association study is currently one of the most effective methods for identifying polymorphisms and loci associated with economically significant traits productive animals. The article presents data obtained during a genome-wide “live weight” indicator in Severocavcazskaya sheep breed. Animal genotyping was carried out using Ovine Infinium HD BeadChip 600K DNA biochips. Genotyping quality control, as well analysis, performed PLINK V.1.07 software. Visualization plotting were QQman...
Abstract The purpose of the study was to research activity nucleolar organizers blood lymphocytes in large white breed pigs postnatal ontogenesis and during pregnancy. prepared smears were stained with silver nitrate (AgNO3) additionally azur eosin. By measuring parameters nucleus organizers’ (AgNORs), changes morphological functional state protein synthesizing apparatus evaluated. It found that area lymphocyte nuclei newborn piglets 83.72±3.37 mm 2 . age three months, significantly...