A5011519954- RNA modifications and cancer
- MicroRNA in disease regulation
- Cancer-related Molecular Pathways
- Multiple Myeloma Research and Treatments
- Cancer-related gene regulation
- Epigenetics and DNA Methylation
- Cancer-related molecular mechanisms research
- Toxic Organic Pollutants Impact
- Ubiquitin and proteasome pathways
- Genomics and Chromatin Dynamics
- Protein Degradation and Inhibitors
- Circular RNAs in diseases
- Endoplasmic Reticulum Stress and Disease
- Peroxisome Proliferator-Activated Receptors
- Radiopharmaceutical Chemistry and Applications
- Phonetics and Phonology Research
- Cancer Genomics and Diagnostics
- Chemical Synthesis and Analysis
- Congenital Ear and Nasal Anomalies
- Lymphoma Diagnosis and Treatment
- Carcinogens and Genotoxicity Assessment
- DNA Repair Mechanisms
- Galectins and Cancer Biology
- Head and Neck Anomalies
- RNA Interference and Gene Delivery
Institute of Human Genetics
2016-2025
Polish Academy of Sciences
2015-2025
In this study, we analyzed the expression profile of four genes (CCNA2, CCNB1, CCNB2, and CDK1) in laryngeal squamous cell carcinoma (LSCC) lines tumor samples. With application microarray platform, have shown overexpression these all LSCC samples comparison to non-cancer controls from head neck region. We selected CDK1 for further analysis, due its leading role cycle regulation. It is a member Ser/Thr protein kinase family proven oncogenic properties. The results obtained were confirmed...
The aryl hydrocarbon receptor (AhR) mediates a variety of biological responses to ubiquitous environmental pollutants. In this study, the effects administration β-naphthoflavone (BNF), potent AhR ligand, on expression AhR-dependent genes were examined by microarray and qPCR analysis in both, differentiated undifferentiated HepaRG cell lines. To prove that BNF-induced changes investigated indeed AhR-dependent, we knock down stable transfection cells with shRNA. Regardless genetical identity,...
Background Laryngeal squamous cell carcinoma (LSCC) is the most common group among head and neck cancers. LSCC characterized by a high incidence in Europe. With aim of better understanding its genetic background we performed global miRNA expression profiling lines primary specimens. By this approach identified cohort 33 upregulated 9 downregulated genes as compared to epithelial no tumor controls. Results Within overexpression novel miR-1290 gene not reported context before. Using combined...
Larynx squamous cell carcinoma (LSCC) is characterized by complex genotypes, with numerous abnormalities in various genes. Despite the progress diagnosis and treatment of this disease, 5-year survival rates remain unsatisfactory. Therefore, extended studies are conducted, aim to find genes, potentially implicated cancer. In study, we focus on FAM107A (3p14.3) gene, since found its significantly reduced expression LSCC microarray profiling (Affymetrix U133 Plus 2.0 array). By RT-PCR have...
DNA methylation was shown previously to be a crucial mechanism responsible for transcriptional deregulation in the pathogenesis of classical Hodgkin lymphoma (cHL). To identify epigenetically inactivated miRNAs cHL, we have analyzed set downregulated cHL cell lines using bisulfite pyrosequencing. We focused on with promoter regions located within or <1000 bp from CpG island. Most promising candidate were further studied primary and Reed-Sternberg (HRS) cells obtained by laser capture...
Laryngeal squamous cell carcinoma is a major medical problem worldwide. Although our understanding of genetic changes and their consequences in laryngeal cancer has opened new therapeutic pathways over the years, diagnostic as well treatment options still need to be improved. In previous study, we identified CRKL (22q11) novel putative oncogene overexpressed amplified subset LSCC tumors lines. Here analyze what extent DNA copy number gains correlate with higher expression protein by...
An abstract is not available for this content. As you have access to content, full HTML content provided on page. A PDF of also in through the ‘Save PDF’ action button.
Introduction: We previously performed global miRNA expression profiling of cHL vs. non-Hodgkin lymphoma (NHL) cell lines and sorted germinal centre B-cells (GCB). In the group 79 deregulated miRNAs (p < 0.05) we became particularly interested in upregulated miR-23a-3p, a potential oncomiR, predicted to regulate TNFAIP3 downregulated miR-148a-3p, tumor suppressor miRNA, IL15. Therefore, aimed decipher role miR-23a-3p miR-148a-3p pathogenesis. Methods: Expression analyses were with TaqMan...
Introduction Laryngeal cancer (LSCC) is one of the most common tumours among head and neck squamous cell carcinomas. Recently, analysis meaning epigenetic alterations in LSCC development has gained momentum. In this field we have shown recurrent overexpression miR-1290 LSCC. Therefore, study aim at clarifying oncomiR functionality by analysing its interaction with transcriptional modulator MAF – a putative suppressor gene. Material methods The identification was based on global miRNA...
Background: Proteasome inhibitors (PIs) are among most potent and widely-used class of drugs in multiple myeloma (MM) treatment. One the main challenges MM therapy is acquired resistance to drugs, PIs no exemption from this phenomenon. Several theories were proposed describe mechanisms responsible for commonly used – bortezomib carfilzomib. promising explanations involves modulation unfolded protein response (UPR) pathway. The pathway initiated by conformational changes three sensor proteins...