A5052793075- Mass Spectrometry Techniques and Applications
- Ion-surface interactions and analysis
- Atomic and Molecular Physics
- Mycobacterium research and diagnosis
- Tuberculosis Research and Epidemiology
- RNA and protein synthesis mechanisms
- Analytical Chemistry and Chromatography
- Diverse Scientific Research Studies
- Medicinal Plants and Neuroprotection
- Bioeconomy and Sustainability Development
- Genetics, Aging, and Longevity in Model Organisms
- Science, Research, and Medicine
- Nuclear Physics and Applications
- Phytochemicals and Medicinal Plants
- Copper Interconnects and Reliability
- Analytical chemistry methods development
- Semiconductor materials and devices
- ZnO doping and properties
Maastricht University
2020-2025
G.S. Science, Arts And Commerce College
2024
University of Delaware
2006
ABSTRACT Rationale To perform native mass spectrometry (MS) studies, there are a limited number of volatile and electrospray ionization (ESI)‐MS compatible solutions, such as ammonium bicarbonate acetate (AA). These solutions could induce the unfolding proteins due to formation CO 2 bubbles or induced acidification during ESI. Hence, it was important introduce buffer suitable preserve form while simulating physiological conditions. Methods The 4‐ethylmorpholinium/acetate (4EM/A) compared AA...
We discuss the design, development, and evaluation of an Orbitrap/time-of-flight (TOF) mass spectrometry (MS)-based instrument with integrated UV photodissociation (UVPD) time/mass-to-charge ratio (m/z)-resolved imaging for comprehensive study higher-order molecular structure macromolecular assemblies (MMAs). A bespoke TOF analyzer has been coupled to higher-energy collisional dissociation cell ultrahigh range hybrid quadrupole-Orbitrap MS. 193 nm excimer laser was employed photofragment MMA...
ESX-1 is a major virulence factor of Mycobacterium tuberculosis, secretion machinery directly involved in the survival microorganism from immune system defence. It disrupts phagosome membrane host cell through contact-dependent mechanism. Recently, structure inner-membrane core complex homologous ESX-3 and ESX-5 was resolved; however, elements outer or those acting on are unknown. Protein substrates might form this missing element. Here, we describe oligomerisation process substrate EspB,...
Native mass spectrometry (native MS) has emerged as a powerful technique to study the structure and stoichiometry of large protein complexes. Traditionally, native MS been performed on modified time-of-flight (TOF) systems combined with detectors that do not provide information arrival coordinates each ion at detector. In this study, we describe implementation Timepix (TPX) pixelated detector orthogonal TOF (O-TOF) spectrometer for analysis imaging unique experimental setup, have used impact...
Aim: The present investigation was undertaken to synthesize Cobalt and copper nanoparticles (NPs) using Limonia acidissima L. leaf extract test their few medicinal properties like antimicrobial antioxidant anti-diabetic activities. Methodology: plant leaves were collected from the vicinity of College study completed in 11 months. hot aqueous soxhlet ethyl acetate as solvent prepared. Phytochemical analysis showed presence Tannins, Flavonoids, Alkaloids absence Phenols. then used make Copper...
Abstract Time‐of‐flight (TOF) systems are one of the most widely used mass analyzers in native spectrometry (nMS) for analysis non‐covalent multiply charged bio‐macromolecular assemblies (MMAs). Typically, microchannel plates (MCPs) employed high ion detection TOF MS. MCPs well known their reduced efficiency when impinged by large slow moving ions. Here, a position‐ and time‐sensitive Timepix (TPX) detector has been added to back dual MCP stack study key factors that affect performance MMA...
The Timepix (TPX) is a position- and time-sensitive pixelated charge detector that can be coupled with time-of-flight mass spectrometry (TOF MS) in combination microchannel plates (MCPs) for the spatially temporally resolved detection of biomolecules. Earlier generation TPX detectors used previous studies were limited by moderate time resolution (at best 10 ns) single-stop each pixel hampered ions high mass-to-charge (m/z) values at occupancies. In this study, we have an MCP-phosphor...
Abstract We present a novel method enabling the infrared laser desorption and electrospray ionisation (ESI) of protein complexes in their native state. Using this method, we demonstrate surprising generation intact, multiply charged ions myoglobin, non‐covalent haemoglobin complex, intact immunoglobulin G antibody states. The observation surviving population is characteristic low internal energy build‐up experienced during both from solution subsequent ionisation. Compared to conventional...
Electrical bias-temperature stress measurements were performed on copper oxide/SiO2/silicon metal-oxide-semiconductor capacitors to measure the oxygen partial pressure dependence of ion transport rates through SiO2 layer. Both copper(I) oxides (Cu2O) and copper(II) (CuO) investigated, oxide film stoichiometry was characterized by glancing incidence x-ray diffraction photoelectron spectroscopy. At relatively high pressures, is observed for both Cu2O∕SiO2∕Si CuO∕SiO2∕Si, activation energies...
Abstract ESX-1 is a major virulence factor of Mycobacterium tuberculosis , secretion machinery directly involved in the survival microorganism from immune system defence. It disrupts phagosome membrane host cell through contact-dependent mechanism. Recently, structure inner-membrane core complex homologous ESX-3 and ESX-5 was resolved; however, elements outer or those acting on are unknown. Protein substrates might form this missing element. Here, we describe oligomerisation process...
The Cover Feature shows a single laser ablation electrospray ionisation event for mass spectrometric analysis of protein complexes. infrared ejects intact complexes containing droplets from sample surface, to interact with an orthogonal nebula charged droplets. These charges are transferred the at atmospheric pressure before entering spectrometer inlet analysis. More information can be found in Communication by R. M. A. Heeren and co-workers.