A5066787290- RNA Research and Splicing
- Genetics, Aging, and Longevity in Model Organisms
- RNA modifications and cancer
- CRISPR and Genetic Engineering
- RNA and protein synthesis mechanisms
- RNA regulation and disease
- Bacterial Genetics and Biotechnology
- Fungal and yeast genetics research
- Astro and Planetary Science
- Genomics and Chromatin Dynamics
- Pluripotent Stem Cells Research
- Ionosphere and magnetosphere dynamics
University of Wisconsin–Madison
2018-2025
MRC Laboratory of Molecular Biology
2024-2025
University of Michigan
2013-2016
Utah State University Space Dynamics Laboratory
2014
Abstract PUF RNA-binding proteins are broadly conserved stem cell regulators. Nematode maintain germline cells (GSCs) and, with key partner proteins, repress differentiation mRNAs, including gld-1 . Here we report that protein FBF-2 and its LST-1 form a ternary complex represses via pair of adjacent FBF binding elements (FBEs) in 3′UTR. One molecule links two molecules motifs the intrinsically-disordered region; FBE includes well-established ‘canonical’ newly-identified noncanonical FBE....
Like all canonical PUF proteins, C. elegans FBF-2 binds to specific RNAs via tripartite recognition motifs (TRMs). Here we report that an mutant protein cannot bind RNA, is nonetheless biologically active and maintains stem cells. This unexpected result challenges the conventional wisdom RBPs must achieve biological activity. Also unexpectedly, interactions with partner proteins can compensate for loss of RNA-binding. only loses activity when its RNA-binding are both defective. These...
Protein-RNA regulatory networks underpin much of biology. C. elegans FBF-2, a PUF-RNA-binding protein, binds over 1,000 RNAs to govern stem cells and differentiation. FBF-2 interacts with multiple protein partners via key tyrosine, Y479. Here, we investigate the in vivo significance partnerships using Y479A mutant. Occupancy mutant increases or decreases at specific sites across transcriptome, varying RNAs. Germline development also changes fashion: abolishes one function-the sperm-to-oocyte...
Metazoan PUF (Pumilio and FBF) RNA-binding proteins regulate various biological processes, but a common theme across phylogeny is stem cell regulation. In Caenorhabditis elegans, FBF (fem-3 Binding Factor) maintains germline cells regardless of which gamete made, also functions in the process spermatogenesis. We have begun to "disentangle" these roles by asking targets are gamete-independent, as expected for cells, gamete-specific. Specifically, we compared iCLIP binding profiles adults...
PUF RNA-binding proteins are conserved stem cell regulators. Four govern self-renewal of Caenorhabditis elegans germline cells together with two intrinsically disordered proteins, LST-1 and SYGL-1. Based on yeast two-hybrid results, we previously proposed a composite hub in the regulatory network, eight partnerships extensive redundancy. Here, investigate LST-1-PUF SYGL-1-PUF their molecular activities natural context - nematode cells. We confirm specificity to PUFs by co-immunoprecipitation...
Abstract PUF RNA-binding proteins are broadly conserved stem cell regulators. Nematode maintain germline cells (GSCs) and, with key partner proteins, repress differentiation mRNAs, including gld-1 . Here we report that protein FBF-2 and its LST-1 form a ternary complex represses via pair of adjacent binding elements (FBEs) in 3’UTR. One molecule links two molecules motifs the intrinsically-disordered region; FBE includes well-established ‘canonical’ newly-identified noncanonical FBE....
Isolation of endogenous proteins from Saccharomyces cerevisiae has been facilitated by inserting encoding polypeptide affinity tags at the C-termini chromosomal open reading frames (ORFs) using homologous recombination DNA fragments. Tagged protein isolation is limited a number factors, including high cost resins for bulk and low concentration ligands on resin surface, leading to efficiencies trapping contaminants. To address this, we have created recombinant "CelTag" construct which PCR...
Abstract PUF RNA-binding proteins are conserved stem cell regulators. Four govern self-renewal of C. elegans germline cells together with two intrinsically disordered proteins, LST-1 and SYGL-1. Based on yeast two-hybrid results, we proposed a composite hub in the regulatory network, eight partnerships extensive redundancy. Here, investigate LST-1–PUF SYGL-1–PUF their molecular activities natural context – nematode cells. We confirm specificity to PUFs by co-immunoprecipitation show that an...
Abstract Like all canonical PUF proteins, C. elegans FBF-2 binds to specific RNAs via tripartite recognition motifs (TRMs). Here we report that an mutant protein cannot bind RNA, is nonetheless biologically active and maintains stem cells. This unexpected result challenges the conventional wisdom RBPs must achieve biological activity. Also unexpectedly, interactions with partner proteins can compensate for loss of RNA-binding. only loses activity when its RNA-binding are both defective....
The Auroral Spatial Structures Probe (ASSP) is a NASA sounding rocket mission to be launched in the late January 2015 time frame that will used study both spatial and temporal small scale variation of electric magnetic fields during active aurora just before onset an auroral sub-storm. This accomplished through use constellation payloads separate relative each other throughout flight.
Abstract Isolation of endogenous proteins from Saccharomyces cerevisiae has been facilitated by inserting encoding polypeptide affinity tags at the C-termini chromosomal open reading frames (ORFs) using homologous recombination DNA fragments. The tagged protein isolation is limited a number factors, including high cost resins for bulk and low concentration ligands on resin surface, leading to efficiencies trapping contaminants. To address this we have created recombinant “CelTag” construct...
Abstract Metazoan PUF (Pumilio and FBF) RNA-binding proteins regulate various biological processes, but a common theme across phylogeny is stem cell regulation. In Caenorhabditis elegans , FBF ( fem-3 Binding Factor) maintains germline cells regardless of which gamete made, also functions in the process spermatogenesis. We have begun to “disentangle” these roles by asking targets are gamete-independent, as expected for cells, gamete-specific. Specifically, we compared iCLIP binding profiles...