A5101792447- RNA Research and Splicing
- RNA modifications and cancer
- RNA and protein synthesis mechanisms
- Cancer-related gene regulation
- Genetics, Aging, and Longevity in Model Organisms
- Genomics and Chromatin Dynamics
- Epigenetics and DNA Methylation
- RNA regulation and disease
- Peroxisome Proliferator-Activated Receptors
- Thin-Film Transistor Technologies
- CRISPR and Genetic Engineering
- Adipose Tissue and Metabolism
- Advanced Surface Polishing Techniques
- Dietary Effects on Health
- Optimism, Hope, and Well-being
- Cardiomyopathy and Myosin Studies
- Grit, Self-Efficacy, and Motivation
- Plant biochemistry and biosynthesis
- Plant and Fungal Interactions Research
- Insect Resistance and Genetics
- Circular RNAs in diseases
- Plant Gene Expression Analysis
- Sphingolipid Metabolism and Signaling
- Reproductive Biology and Fertility
- Molecular Biology Techniques and Applications
National Institute of Environmental Health Sciences
2011-2025
National Institutes of Health
2011-2025
Chinese University of Hong Kong, Shenzhen
2024
Soochow University
2011-2024
Chengdu University of Traditional Chinese Medicine
2022
Shanxi Agricultural University
2021
Chinese Academy of Sciences
2014-2018
Center for Excellence in Molecular Plant Sciences
2014-2018
University of Chinese Academy of Sciences
2018
Shanghai Institutes for Biological Sciences
2014-2015
Collaboration among the multitude of RNA-binding proteins (RBPs) is ubiquitous, yet our understanding these key regulatory complexes has been limited to single RBPs. We investigated combinatorial translational regulation by Drosophila Pumilio (Pum) and Nanos (Nos), which control development, fertility, neuronal functions. Our results show how specificity one RBP modulated cooperative RNA recognition with a second (Nos) synergistically repress mRNAs. Crystal structures Nos-Pum-RNA reveal that...
Abstract Proteins bind and control mRNAs, directing their localization, translation stability. Members of the PUF family RNA-binding proteins multiple mRNAs in a single cell, play key roles development, stem cell maintenance memory formation. Here we identified mRNA targets S. cerevisiae protein, Puf5p, by ultraviolet-crosslinking-affinity purification high-throughput sequencing (HITS-CLIP). The binding sites recognized Puf5p are diverse, with variable spacer lengths between two specific...
Significance RNA regulation occurs at many levels including processing to mature forms, subcellular localization, and translation. RNA-binding proteins are crucial direct regulate these processes. Pumilio/feminization of XX XO animals (fem)-3 mRNA-binding factor (PUF) formed from eight α-helical repeats [Pumilio (PUM) repeats] that recognize specific mRNA sequences. Previous structural studies revealed characteristic curved structures sequence specificity unique classical PUF proteins. We...
Abstract PUF RNA-binding proteins are broadly conserved stem cell regulators. Nematode maintain germline cells (GSCs) and, with key partner proteins, repress differentiation mRNAs, including gld-1 . Here we report that protein FBF-2 and its LST-1 form a ternary complex represses via pair of adjacent FBF binding elements (FBEs) in 3′UTR. One molecule links two molecules motifs the intrinsically-disordered region; FBE includes well-established ‘canonical’ newly-identified noncanonical FBE....
Aims: This study aimed to explore the relationship between growth mindset and job satisfaction among Chinese nurses, examine underlying mediating role of grit self‐efficacy in this relationship. Background: As a long‐standing research topic nursing management, plays an essential patient care, healthcare organizations, nurses’ career planning development. Therefore, it is extremely important psychosocial factors that contribute satisfaction. Design: A descriptive, cross‐sectional survey...
Stacking interactions between amino acids and bases are common in RNA–protein interactions. Many proteins that regulate mRNAs interact with single-stranded RNA elements the 3′ UTR (3′-untranslated region) of their targets. PUF exemplary. Here we focus on complexes formed a Caenorhabditis elegans protein, FBF, its cognate RNAs. particularly prominent involve every base recognition element. To assess contribution stacking to formation complex, combine vivo selection experiments site-directed...
MBD4 is a member of the methyl-CpG-binding protein family. It contains two DNA binding domains, an amino-proximal methyl-CpG domain (MBD) and C-terminal mismatch-specific glycosylase domain. Limited <i>in vitro</i> proteolysis mouse yields stable fragments: 139-residue fragment including MBD, other 155-residue Here we show that latter active as on duplex containing G:T mismatch within CpG sequence context. The crystal structure confirmed helix-hairpin-helix superfamily. site situated in...
mRNA control hinges on the specificity and affinity of proteins for their RNA binding sites. Regulatory must bind own sites reject even closely related noncognate In PUF [Pumilio fem-3 factor (FBF)] family proteins, individual discriminate differences in length sequence sites, allowing each to a distinct battery mRNAs. Here, we show that despite these differences, pattern interactions is conserved among proteins: two ends protein make critical contacts with Despite this "two-handed"...
Numerous factors direct eukaryotic ribosome biogenesis, and defects in a single assembly factor may be lethal or produce tissue-specific human ribosomopathies. Pre-ribosomal RNAs (pre-rRNAs) must processed stepwise at the correct subcellular locations to mature rRNAs. Nop9 is conserved small ribosomal subunit biogenesis factor, essential yeast. Here we report 2.1-Å crystal structure of small-angle X-ray-scattering model Nop9:RNA complex that reveals 'C'-shaped fold formed from 11 Pumilio...
In the Caenorhabditis elegans germline, fem-3 Binding Factor (FBF) partners with LST-1 to maintain stem cells. A crystal structure of an FBF-2/LST-1/RNA complex revealed that FBF-2 recognizes a short RNA motif different from characteristic 9-nt FBF binding element, and compact recognition coincided curvature changes in scaffold. Previously, we engineered favor shorter motifs without change (Bhat et al., 2019). vitro selection RNAs bound by suggested sequence specificity central region...
Abstract PUF proteins are characterized by globular RNA-binding domains. They also interact with partner that modulate their activities. Caenorhabditis elegans protein fem-3 binding factor-2 (FBF-2) partners intrinsically disordered Lateral Signaling Target-1 (LST-1) to regulate target mRNAs in germline stem cells. Here, we report an region (IDR) at the C-terminus of FBF-2 autoinhibits its affinity increasing off rate for RNA binding. Moreover, C-terminal interacts domain same site where...
PUF proteins, named for Drosophila Pumilio (PUM) and Caenorhabditis elegans fem-3-binding factor (FBF), recognize specific sequences in the mRNAs they bind control. RNA binding by classical proteins is mediated a characteristic PUM homology domain (PUM-HD). The Puf1 Puf2 possess distinct architecture comprise highly conserved subfamily among fungal species. Puf1/Puf2 contain two types of RNA-binding domain: divergent PUM-HD an recognition motif (RRM). They RNAs containing UAAU motifs, often...
Memo (mediator of ErbB2-driven cell motility) is a 297-amino-acid protein recently shown to co-precipitate with the C terminus ErbB2 and be required for motility. not homologous any known signaling proteins, how it mediates signals known. To provide molecular basis understanding function, we have determined report here 2.1A crystal structure human show class III nonheme iron-dependent dioxygenases, structural that now includes zinc-binding unknown function. No metal binding or enzymatic...
PUF (PUmilio/FBF) RNA-binding proteins recognize distinct elements. In C. elegans, PUF-8 binds to an 8-nt motif and restricts proliferation in the germline. Conversely, FBF-2 recognizes a 9-nt element promotes mitosis. To understand how divergence relates biological function, we first determined crystal structure of PUF-8. Comparison this that revealed major difference central repeat. We devised modified yeast 3-hybrid screen identify mutations confer recognition FBF-2. identified several...
Abstract PUF RNA-binding proteins are broadly conserved stem cell regulators. Nematode maintain germline cells (GSCs) and, with key partner proteins, repress differentiation mRNAs, including gld-1 . Here we report that protein FBF-2 and its LST-1 form a ternary complex represses via pair of adjacent binding elements (FBEs) in 3’UTR. One molecule links two molecules motifs the intrinsically-disordered region; FBE includes well-established ‘canonical’ newly-identified noncanonical FBE....
SRm160 is an SR-like protein implicated in multiple steps of RNA processing and nucleocytoplasmic export. Although its biochemical functions have been extensively described, genetic interactions potential participation signaling pathways remain largely unknown, despite the fact that it highly phosphorylated both mammalian cells Drosophila. To begin elucidating role developmental processes, we characterized mutant overexpression phenotypes Drosophila their with locus encoding LAMMER kinase,...
In C. elegans, PUF proteins promote germline stem cell self-renewal. Their functions hinge on partnerships with two that are redundantly required for maintenance. Here we focus understanding how the essential partner protein, LST-1, modulates mRNA regulation by FBF-2. LST-1 contains nonidentical sites of interaction FBF-2, A and B. Our crystal structures complexes A, RNA visualize FBF-2 associates versus One commonality is contacts conserved lysine leucine side chains in KxxL motifs key...