A5009411311- Genetic Syndromes and Imprinting
- Epigenetics and DNA Methylation
- Animal Genetics and Reproduction
- Sperm and Testicular Function
- Reproductive Biology and Fertility
- CRISPR and Genetic Engineering
- Cancer-related molecular mechanisms research
- Mesenchymal stem cell research
- Prenatal Screening and Diagnostics
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Pluripotent Stem Cells Research
- Adipose Tissue and Metabolism
- Chromosomal and Genetic Variations
- Nerve injury and regeneration
- Lysosomal Storage Disorders Research
- Histone Deacetylase Inhibitors Research
- MicroRNA in disease regulation
- RNA modifications and cancer
- Renal and related cancers
- Diet and metabolism studies
- Tissue Engineering and Regenerative Medicine
- Metaheuristic Optimization Algorithms Research
- Liver Disease Diagnosis and Treatment
- Mitochondrial Function and Pathology
- Virus-based gene therapy research
National Taiwan University
2015-2025
China Pharmaceutical University
2025
Agricultural Biotechnology Research Center, Academia Sinica
2012-2024
First Affiliated Hospital of Fujian Medical University
2024
Fujian Medical University
2024
Genomics Research Center, Academia Sinica
2024
Academia Sinica
2018-2020
Biotechnology Research Center
2018
TaiGen Biotechnology (Taiwan)
2018
Czech Academy of Sciences, Institute of Biotechnology
2012
microRNAs (or miRNAs) are small noncoding RNAs (21 to 25 nucleotides) that processed from longer hairpin RNA precursors and believed be involved in a wide range of developmental cellular processes, by either repressing translation or triggering mRNA degradation (RNA interference). By using computer-assisted approach, we have identified 46 potential miRNA genes located the human imprinted 14q32 domain, 40 which organized as large cluster. Although some these clustered appear encoded...
Pluripotent stem cells are increasingly used to build therapeutic models, including the transplantation of neural progenitors derived from human embryonic (hESCs). Recently, long non-coding RNAs (lncRNAs), delta-like homolog 1 gene and type III iodothyronine deiodinase (DLK1-DIO3) imprinted locus-derived maternally expressed 3 (MEG3), were found be during development. The deregulation these lncRNAs is associated with various neurological diseases. locus DLK1-DIO3 encodes abundant (ncRNAs)...
Genomic imprinting is a normal process that causes genes to be expressed according parental origin. The selective advantage conferred by not understood but hypothesised act on dosage-critical genes. Here, we report unique model in which the consequences of single, double, and triple dosage imprinted Dlk1/Pref1, normally repressed maternally inherited chromosome, can assessed growing embryo. BAC-transgenic mice were generated over-express Dlk1 from endogenous regulators at all sites embryonic...
Genomic imprinting is an epigenetic mechanism controlling parental-origin-specific gene expression. Perturbing the parental origin of distal portion mouse chromosome 12 causes alterations in dosage imprinted genes resulting embryonic lethality and developmental abnormalities both embryo placenta. A 1 Mb domain identified on contains three paternally expressed protein-coding multiple non-coding RNA genes, including snoRNAs microRNAs,expressed from maternally inherited chromosome. An...
The mammalian imprinted Dlk1-Dio3 locus produces multiple long non-coding RNAs (lncRNAs) from the maternally inherited allele, including Meg3 (i.e., Gtl2) in genome. Although this has well-characterized functions stem cell and tumor contexts, its role during neural development is unknown. By profiling types at each stage of embryonic cell-derived motor neurons (ESC~MNs) that recapitulate spinal cord development, we uncovered lncRNAs expressed are predominantly gradually enriched rostral...
The ability of adult stem cells to reside in a quiescent state is crucial for preventing premature exhaustion the cell pool. However, intrinsic epigenetic factors that regulate spermatogonial quiescence are largely unknown. Here, we investigate mice how DNA methyltransferase 3-like (DNMT3L), an regulator important interpreting chromatin context and facilitating de novo methylation, sustains long-term male germ We demonstrated cell-enriched THY1+ stem/progenitor (SPCs) constituted...
Idiopathic pulmonary fibrosis is incurable, and its progression difficult to control thus can lead deterioration. Pan-histone deacetylase inhibitors such as SAHA have shown potential for modulating yet with off-target effects. Therefore, selective HDAC would be beneficial reducing side Toward this goal, we designed synthesized 24 novel HDAC6, HDAC8, or dual HDAC6/8 established a two-stage screening platform rapidly screen that effectively mitigate TGF-β-induced fibrosis. The first stage...
In mammals, imprinted gene expression results from the sex-specific methylation of control regions (ICRs) in parental germlines. Imprinting is linked to therian reproduction, that is, placenta and imprinting emerged at roughly same time potentially co-evolved. We assessed transcriptome-wide ontology effect maternally versus paternally methylated ICRs developmental stage setting chorioallantoic mouse (8.5dpc), using two models deficiency including completely imprint-free embryos. Paternal...
SERPINE2, one of the potent serine protease inhibitors that modulates activity plasminogen activator and thrombin, is implicated in many biological processes. In present study, we purified SERPINE2 from mouse seminal vesicle secretion (SVS), using liquid chromatography identified it by chromatography/tandem mass spectrometry, showed inhibitory against urokinase-type activator. was expressed predominantly vesicles among murine male reproductive tissues. It immunolocalized to SVS mucosal...
Epigenetic regulation by histone deacetylases (HDACs) in Schwann cells (SCs) after injury facilitates them to undergo de- and redifferentiation processes necessary support various stages of nerve repair. Although de-differentiation activates the synthesis secretion inflammatory cytokines SCs initiate an immune response during repair, changes either timing or duration prolonged inflammation mediated can affect later associated with repair regeneration. Limited studies have investigated...
The failure of peripheral nerve regeneration is often associated with the inability to generate a permissive molecular and cellular microenvironment for repair. Autologous therapies, such as platelet-rich plasma (PRP) or its derivative growth factors (PRGF), may improve via unknown mechanistic roles actions in macrophage polarization. In current study, we hypothesize that excessive prolonged inflammation might result pro-inflammatory M1 transit anti-inflammatory M2 macrophages large defects....
Some studies have revealed the effects of environmental arsenic exposure on learning and memory ability male rats its epigenetic mechanism, found that led to downregulation cognitive-related gene expression, change histone modification, then caused neurotoxicity, paternity cross-generation impairment in F2 generation rats. In fact, addition harsh environment, providing a favorable environment for mice can also show phenomenon cross-generational inheritance their offspringVarious indicate...
Abstract Background Microenvironmental alterations induce significant genetic and epigenetic changes in stem cells. Mitochondria, essential for regenerative capabilities, provide the necessary energy cell function. However, specific roles of histone modifications mitochondrial dynamics human adipose-derived cells (ASCs) during morphological transformations remain poorly understood. In this study, we aim to elucidate mechanisms by which ASC sphere formation enhances function, delivery, rescue...
Serum amyloid A (SAA) is not only an apolipoprotein, but also a member of the adipokine family with potential to enhance lipolysis. The purpose this study was explore how SAA facilitates lipolysis in porcine adipocytes. We found that increased phosphorylation perilipin and hormone-sensitive lipase (HSL) after 12-h treatment decreased expression 24-h treatment, these effects were prevented by extracellular signal-regulated kinase (ERK) or protein (PKA) inhibitors primary adipocyte cell...
Background While bone marrow (BM) is a rich source of mesenchymal stem cells (MSCs), previous studies have shown that MSCs derived from mouse BM (BMMSCs) were difficult to manipulate as compared other species. The objective this study was find an alternative murine could provide sufficient MSCs. Methodology/Principal Findings In study, we described novel type migrates directly the epiphysis in culture. Epiphysis-derived (EMSCs) be extensively expanded plastic adherent culture, and they had...
This study aimed to isolate, culture, and characterize duck primordial germ cells (PGCs) compare these with chicken PGCs. We first cultured Muscovy (Cairina moschata) circulating PGCs gonadal (gPGCs) in the modified serum-containing medium used amplify gPGCs were found proliferate better serum-free chemically defined than medium. Thereafter, similarly isolated from 2 other breeds, Pekin (Anas platyrhynchos) hybrid mule (C. moschata × A. platyrhynchos), amplified for a limited period of time...