A5060660072- Epigenetics and DNA Methylation
- Cancer-related gene regulation
- RNA modifications and cancer
- Genomics and Chromatin Dynamics
- Virus-based gene therapy research
- Viral gastroenteritis research and epidemiology
- RNA Interference and Gene Delivery
- Histone Deacetylase Inhibitors Research
- Advanced biosensing and bioanalysis techniques
- Cystic Fibrosis Research Advances
- Cancer-related molecular mechanisms research
- Bacteriophages and microbial interactions
- Biochemical effects in animals
- Enzyme Structure and Function
- RNA and protein synthesis mechanisms
- DNA and Nucleic Acid Chemistry
- Graphene and Nanomaterials Applications
- Nanoparticles: synthesis and applications
- Computational Drug Discovery Methods
- Pain Management and Opioid Use
- Ferroptosis and cancer prognosis
- Pharmacological Effects and Toxicity Studies
- Heme Oxygenase-1 and Carbon Monoxide
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Cancer Genomics and Diagnostics
Shandong Provincial Hospital
2023-2024
Tianjin Anding Hospital
2024
Tianjin Medical University
2016-2024
Wayne State University
2015-2024
Fuzhou University
2017-2024
First Affiliated Hospital of Xi'an Jiaotong University
2022-2024
Shandong First Medical University
2023-2024
First Affiliated Hospital of GuangXi Medical University
2023
Guangxi Medical University
2023
Shandong University
2023
Various agents, including drugs as well nonmolecular stimuli, induce alterations in the physicochemical properties of proteins cell lysates, living cells, and organisms. These can be probed by applying a stability- solubility-modifying factor, such elevated temperature, to varying degree. As second dimension variation, drug concentration or agent intensity/concentration used. Compared standard approaches where curves are fitted protein solubility data acquired at different temperatures...
Significance Histone-modifying enzymes play an important role in regulating chromatin-associated processes such as transcription. In addition to modifying histones, these control gene expression through nonhistone proteins, including transcription factors. this study, we show that SET and MYND domain containing 2 (SMYD2), a histone H3K4 H3K36 methyltransferase, directly methylates estrogen receptor alpha (ERα) protein at lysine 266 represses ERα transactivation activity. Upon activation,...
Abstract Peritoneal metastasis (PM) has a suppressive tumor immune microenvironment (TIME) that limits the effects of immunotherapy. This study aimed to investigate immunomodulatory intraperitoneal administration IL-33, cytokine is reported potentiate antitumor immunity and inhibit metastasis. We found survival was significantly prolonged in patients with high IL-33 mRNA expression. In immunocompetent mice, could induce celiac inflammatory environment, activate immunologic effector cells,...
Methylation of Lys79 on histone H3 by Dot1p is important for gene silencing. The elongated structure the conserved core yeast contains an N-terminal helical domain and a seven-stranded catalytic that harbors binding site methyl-donor active pocket sided with hydrophobic residues. S-adenosyl-L-homocysteine exhibits extended conformation distinct from folded observed in structures SET lysine methyltransferases. A asparagine (Asn479), located at bottom pocket, suggests mechanism similar to...
SmyD1 is a cardiac- and muscle-specific histone methyltransferase that methylates H3 at lysine 4 regulates gene transcription in early heart development. The unique domain structure characterized by "split" SET domain, conserved MYND zinc finger, novel C-terminal (CTD) distinguishes from other containing methyltransferases. Here we report the crystal of full-length complex with cofactor analog sinefungin 2.3 Å. reveals folds into wrench-shaped two thick "grips" separated large, deep concave...
Oxidative stress is a key factor implicated in the development of diabetic neuropathy. This study evaluates prophylactic and antinociceptive effects antioxidant coenzyme Q10 (CoQ10) on diabetes-induced neuropathic pain mouse model.Total 56 mice with type 1 diabetes induced by streptozotocin were used, 20 normal used as control. Mechanical thermal nociceptive behavioral assays applied to evaluate pain. Tissue lipid peroxidation, immunohistochemistry, reverse transcription, polymerase chain...
In Brief BACKGROUND: Previous studies have associated the catechol-O-methyltransferase (COMT) enzyme rs4680 polymorphism with opioid consumption in treatment of chronic cancer pain. this study, we evaluated association between COMT and rs4818 polymorphisms acute postoperative period after a nephrectomy. METHODS: Opioid pain scores were 152 patients for 48 hours The genotype each patient was determined using polymerase chain reaction on DNA extracted from blood samples. genotypes general...
The microRNA profiles within living cells are informative for diagnosis and prognosis of human cancers. In the present work, we developed a new sensing strategy based on branched DNA junction-enhanced isothermal circular strand displacement polymerization (B-ICSDP) detection intracellular imaging microRNAs in interest. A template consisting three repetitive fragments serves as scaffold self-assembly sophisticated signaling probes, resulting shrunk junction. Target triggers opening molecular...
To date, X-ray crystallography remains the gold standard for determination of macromolecular structure and protein substrate interactions. However, unpredictability obtaining a crystal limiting factor continues to be bottleneck in determining structures. A vast amount research has been conducted order circumvent this issue with limited success. No single method proven guarantee crystallization all proteins. techniques using antibody fragments, lipids, carrier proteins, even mutagenesis...
Histone deacetylase 6 (HDAC6), a class IIb HDAC, plays an important role in many biological and pathological processes. Previously, we found that ERK1, downstream kinase the mitogen-activated protein signaling pathway, phosphorylates HDAC6, thereby increasing HDAC6-mediated deacetylation of α-tubulin. However, whether HDAC6 reciprocally modulates ERK1 activity is unknown. Here, report both -2 are acetylated promotes via deacetylation. Briefly, physically interact with HDAC6. Endogenous...
SmyD2 belongs to a new class of chromatin regulators that control gene expression in heart development and tumorigenesis. Besides methylation histone H3 K4, can methylate non-histone targets including p53 the retinoblastoma tumor suppressor. The methyltransferase activity SmyD proteins has been proposed be regulated by autoinhibition via intra- interdomain bending conserved C-terminal domain (CTD). However, there no direct evidence conformational change CTD. Here, we report two crystal...
Abstract Sponge gourd ( Luffa cylindrica (L.) Roem.) or luffa is a diploid herbaceous plant with 26 chromosomes (2n = 26) and belongs to the family Cucurbitaceae. To address limited knowledge of genome species, chromosome‐level L. was assembled analysed using PacBio long reads Hi‐C data. We combined data draft assembly generate chromosome‐length scaffolds. Thirteen scaffolds corresponding 13 were from 1,156 contigs final size 669 Mb contig N50 5 scaffold 53 Mb. After removing redundant...
Abstract Sustained androgen receptor (AR) signaling and apoptosis evasion are among the main hurdles of castration‐resistant prostate cancer (CRPC) treatment. We designed synthesized isothiocyanate (ITC)‐containing hybrid AR antagonist (ITC‐ARi) rationally combined ITC‐ARi with GSH synthesis inhibitor buthionine sulfoximine (BSO) to efficiently downregulate AR/AR splice variant induce ferroptosis in CRPC cells. The representative 13 is an ligand that contains N ‐acetyl cysteine‐masked ITC...
Abstract Aberrant expression of the Forkhead box transcription factor, FOXQ1, is a prevalent mechanism epithelial-mesenchymal transition (EMT) and metastasis in multiple carcinoma types. However, it remains unknown how FOXQ1 regulates gene expression. Here, we report that initiates EMT by recruiting MLL/KMT2 histone methyltransferase complex as transcriptional coactivator. We first establish promoter recognition precedes MLL assembly histone-3 lysine-4 trimethylation within regions critical...
Abstract Background Acetyl-CoA acetyltransferase 2 (ACAT2) is a lipid metabolism enzyme and rarely was researched in epithelial ovarian cancer (EOC). Methods ACAT2 expressions were confirmed two pairs of cell lines (A2780 A2780/DDP, OVCAR8 OVCAR8/DDP) from Gene Expression Omnibus database by bioinformatics analysis, A2780 A2780/DDP quantitative real-time polymerase chain reaction western blotting. Tissue samples stained immunohistochemistry scored for expression. The relationships between...
Recent studies have shown that CYP2D6 acts at critical steps for endogenous morphine biosynthesis. The present study assessed the contribution of genetic polymorphisms, smoking, and other factors on acute severe postoperative pain (linear analog scores ≥8).Two hundred thirty-six female patients were found to adequate information in a previously developed surgical patient database be included this current analysis. Multiple logistic regression analysis was used assess predictors pain. DNA had...